Neutrophil Activation by Recombinant Cytokines

Steinbeck, Marla J.; Roth, James A.

doi:10.1093/clinids/11.4.549

Cited by 177 publications

(78 citation statements)

References 127 publications

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“…In this study we demonstrate a NO-dependent component responsible for P. aeruginosa killing by IFN-␥-treated rat blood neutrophils. Stimulation of PMN with IFN-␥ is known to prime phagocytes for different functions, including enhancement in the production of oxidative toxic metabolites [43]. P. aeruginosa, an essentially aerobic microorganism, has the ability to produce superoxide dismutase and catalase that could protect itself from the deleterious effect of superoxide anion and hydrogen peroxide generated by phagocytes and by its own metabolism [44].…”

Section: Discussionmentioning

confidence: 99%

Induction of NOS in rat blood PMN in vivo and in vitro: modulation by tyrosine kinase and involvement in bactericidal activity

Fierro¹,

Nascimento-DaSilva²,

Arruda³

et al. 1999

Journal of Leukocyte Biology

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Section: Discussionmentioning

confidence: 99%

Induction of NOS in rat blood PMN in vivo and in vitro: modulation by tyrosine kinase and involvement in bactericidal activity

Fierro¹,

Nascimento-DaSilva²,

Arruda³

et al. 1999

Journal of Leukocyte Biology

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“…Since all of the neutrophils in the bloodstream have recently been released from the hematopoietic tissues, the neutrophils are an easily accessible cell type for evaluating factors that may influence leukocyte development. Neutrophil function may be enhanced by several cytokines released during a cell-mediated immune response (31 (29), leave animals with a moderately increased susceptibility to an infectious challenge.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Influence of Potential Toxins on Neutrophil Function

Roth

1993

Toxicol Pathol

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Neutrophils undergo a complex series of events when functioning to control bacterial, viral, and fungal infections. Any factor that interferes with neutrophil production or suppresses neutrophil function will rapidly make the animal more susceptible to infectious diseases. In vitro assays are used to detect and characterize defects in neutrophil function that may be induced by exposure to potential toxins. This paper focuses on important considerations in designing experiments for the evaluation of neutrophil function and interpretation of data.

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“…In healthy volunteers, rhG-CSF has already been found to enhance the migration, phagocytosis, bactericidal activity, and superoxide generation of PMNs in vitro and in vivo (12,20). In an experimental P. aeruginosa infection model in burned mice, it was reported that rhG-CSF administration increased the granulocyte count and improved the survival rate (15).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Opsonophagocytic Dysfunctions in Severely Burned Patients by Luminol‐Dependent Chemiluminescence

Ono

Kunii

Kobayashi

et al. 1993

Microbiology and Immunology

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We compared the luminol-dependent chemiluminescence (CL) response of peripheral blood from severely burned patients with that from normal controls to evaluate the primary defense level against bacterial infection in the patients. The CL was measured upon addition to diluted whole blood of a soluble stimulus, phorbol myristate acetate (PMA) or particulate stimuli such as bacteria or zymosan without special opsonization. In the early post-burn days, the initial rate of whole blood CL induced with the particulate stimulus was much lower than that in the normal controls, whereas the rate was higher when PMA was used as a stimulus. The number of granulocytes in the patients' blood had increased and isolated polymorphonuclear leukocytes (PMNs) from the patients exhibited higher CL responses to the particulate or soluble stimulus as compared with those of normal controls. The results suggest that the PMNs in burn patients were activated and normally mobilized in the early post-burn period but the opsonizing capacity in the blood decreased. In fact, the serum levels of complement, immunoglobulins and fibronectin were found to be lower in the blood from the patients than those from normal controls and a supplement of freshly frozen plasma of human immunoglobulin preparations restored the initial rate of the whole blood CL upon phagocytosis. The prognosis is still poor when severe infection occurs in the patients with decreased CL response of whole blood. Recombinant human granulocyte colonystimulating factor (rhG-CSF) enhanced the CL response of PMNs from burn patients. The administration of rhG-CSF may be useful for decreasing the morbidity of severe infection following burn injury in the near future.

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Neutrophil Activation by Recombinant Cytokines

Cited by 177 publications

References 127 publications

Induction of NOS in rat blood PMN in vivo and in vitro: modulation by tyrosine kinase and involvement in bactericidal activity

Induction of NOS in rat blood PMN in vivo and in vitro: modulation by tyrosine kinase and involvement in bactericidal activity

Evaluation of the Influence of Potential Toxins on Neutrophil Function

Evaluation of Opsonophagocytic Dysfunctions in Severely Burned Patients by Luminol‐Dependent Chemiluminescence

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