Neutrophil and eosinophil involvement of the small bowel in patients with celiac disease and Crohn's disease: Studies on the secretion rate and immunohistochemical localization of granulocyte granule constituents

Hällgren, Roger; Colombel, Jean Fréd́eric; Dahl, Ronald; Fredens, Kjeld; A, Kruse; Jacobsen, N; Venge, Per; Rambaud, J.-C.

doi:10.1016/0002-9343(89)90230-1

Cited by 113 publications

(54 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…18,19 These cytotoxic proteins are found in intestinal fluids and may cause mucosal damage to the digestive tract. 20 In the liver, eosinophils are found in portal tracts during acute liver allograft rejection. [21][22][23] In this context of allograft rejection, in the liver as in the kidney, tissue eosinophils show signs of activation with deposits of cationic protein, 24 and their density has a predictive value for steroid-resistant rejection.…”

Section: Introductionmentioning

confidence: 99%

Activated eosinophils in upper gastrointestinal tract of patients with graft-versus-host disease

Daneshpouy

Socié

Lémann

et al. 2002

Blood

View full text Add to dashboard Cite

Digestive tract damage during graft-versus-host reaction (GVHR) causes high morbidity and mortality. Diagnosis is often late because biopsies are performed when clinical signs are severe and pathologic markers of early inflammatory lesions are lacking. Eosinophils are inflammatory cells, cytotoxic in vitro to digestive epithelium; they are found in biopsy specimens taken during acute flare-ups of inflammatory bowel disease. We performed systematic duodenal biopsies immediately after digestive symptoms occurred and found a digestive GVHR incidence of 73.1% (n ‫؍‬ 93), higher than that found when digestive biopsies were performed immediately after severe clinical signs. Eosinophils were only present when there were histologic signs of GVHR; eosinophil presence correlated with GVHR severity. Electron microscopy with immunogold staining showed pathologic signs of in situ eosinophil activation, such as cytoplasmic granule alterations, and eosinophil peroxidase release in all patients. Interleukin-5 presence in activated eosinophils suggests eosinophil recruitment in digestive GVHR is an autocrine mechanism. Eosinophil density also correlated with GVHR severity, whether in acute or chronic clinical phases. Tissue eosinophils could thus be a marker of acute inflammatory flare-ups in GVHR. Systematic duodenal biopsy performed at the onset of digestive symptoms should allow early GVHR detection, and pathologic signs of GVHR, together with eosinophil density, might help modulate immunosuppressive therapy. (Blood. 2002;99:3033-3040)

show abstract

Section: Introductionmentioning

confidence: 99%

Activated eosinophils in upper gastrointestinal tract of patients with graft-versus-host disease

Daneshpouy

Socié

Lémann

et al. 2002

Blood

View full text Add to dashboard Cite

show abstract

“…The immunoperoxidase method was used with the polyclonal antibodies against ECP, as previously described (21). Conventional controls, as detailed by Sternberger (32), were used for the stains.…”

mentioning

confidence: 99%

“…The mouse monoclonal antibody EG2 was detected by means of the unlabeled bridge technique as described elsewhere (21,33). A mouse monoclonal antibody of the same isotype was used as a control.…”

mentioning

confidence: 99%

Eosinophil activation in systemic sclerosis

Gustafsson

Hällgren

Fredens

et al. 1991

Arthritis & Rheumatism

Self Cite

View full text Add to dashboard Cite

Circulating levels of eosinophil cationic protein (ECP) were increased 4-fold in patients with systemic sclerosis (SSc) compared with those in healthy control subjects. There was no correlation between the ECP concentrations and laboratory indices of inflammatory activity or visceral involvement. Mean ECP levels were higher in patients with a history of occupational exposure to silica, even though patients who had no such history also had ECP levels higher than normal. The patients had increased bronchoalveolar levels of ECP, which correlated with impaired lung functioning. Skin infiltration with activated eosinophils and extracellular deposits of ECP were present in skin biopsy samples from the SSc patients. We conclude that eosinophil activation is part of the inflammatory process in SSc.The etiology of systemic sclerosis (SSc) is unknown although an increased prevalence has been reported in workers exposed to gold and silica. Most pathogenic findings in patients with SSc involve alterations in the vascular system (1-5), immunologic abnormalities (I-3,6-9), and disturbances in the regula-

show abstract

“…These cells are also increased in Crohn disease lesions and also harbour increased capacity of mucosal migration (30). Furthermore, an increased degranulation of these cells has been suggested even in the unaffected intestinal area in Crohn disease patients (31). Interestingly a recent paper showed the potential influence of neutrophil products such as elastase on eosinophil activation and degranulation (32).…”

Section: Discussionmentioning

confidence: 99%

Increased Response of Blood Eosinophils to Various Chemotactic Agents in Quiescent Crohn Disease

Denis¹,

Louis²,

Malaise³

et al. 2001

Scandinavian Journal of Gastroenterology

View full text Add to dashboard Cite

Background: The number of eosinophils is increased in the mucosae of the digestive and the respiratory tracts in Crohn disease, even clinically quiescent. The mechanisms underlying this panmucosal eosinophilia are unknown. Methods: The response of blood eosinophils to various chemotactic agents was assessed in 15 patients with clinically quiescent Crohn disease. The results were compared with 15 healthy controls. After purification, eosinophils were placed in Boyden microchambers and the chemotactic effect of PAF (10 -7 M), RANTES (50ng/ml), IL-5 (0-20ng/ml), IL-8 (0-50ng/ml), Eotaxin (0-50 ng/ml) was evaluated. The number of eosinophils in induced sputum of these Crohn disease patients and controls was also assessed and the correlation between chemotaxis and eosinophil count in induced sputum was studied.

show abstract

Neutrophil and eosinophil involvement of the small bowel in patients with celiac disease and Crohn's disease: Studies on the secretion rate and immunohistochemical localization of granulocyte granule constituents

Cited by 113 publications

References 32 publications

Activated eosinophils in upper gastrointestinal tract of patients with graft-versus-host disease

Activated eosinophils in upper gastrointestinal tract of patients with graft-versus-host disease

Eosinophil activation in systemic sclerosis

Increased Response of Blood Eosinophils to Various Chemotactic Agents in Quiescent Crohn Disease

Contact Info

Product

Resources

About