Neutrophil and macrophage apoptosis in bronchoalveolar lavage fluid from healthy horses and horses with recurrent airway obstruction (RAO)

Niedźwiedź, Artur; Jaworski, Zbigniew; Tykałowski, Bartłomiej; Smiałek, M

doi:10.1186/1746-6148-10-29

Cited by 28 publications

(14 citation statements)

References 31 publications

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“…Lack of the above mentioned data creates difficulties in assessing and comparing the results with those of other authors. In humans, many insights have been made into the pathogenesis of diseases such as rheumatoid arthritis and glomerulonephritis, using the analysis of the levels of circulating immune complexes levels (Nowak-Łoś et al 2013, Navolotskaya 2014, Wener 2014. The incidence of circulating immune complexes has been ascertained for many disease conditions (Haymen 1990, Pandey 2013, Maheswari et al 2014) and the level of circulating immune complexes has been correlated with the activity of certain diseases.…”

Section: Discussionmentioning

confidence: 99%

“…An endoscopy and BAL procedure was performed using methods previously described (Niedzwiedz et al 2014). The amount of the recovered fluid was recorded and BALF for each individual horse was pooled in a sterile specimen cup, placed on ice and processed within 2h after collection.…”

Section: Collection and Cytologic Evaluation Of Balfmentioning

confidence: 99%

“…The elimination of a foreign antigen is carried out by the antibody, complement and phagocyte systems. When an immune complex is formed in the circulation, clearance is affected by cells are acknowledged markers in the diagnosis of inflammatory diseases and provide useful aid in determining the immunopathology of the disease, help in determining the prognosis and are useful during the follow-up (Nowak-Łoś et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Circulating immune complexes and markers of systemic inflammation in RAO-affected horses

Niedźwiedź¹,

Jaworski²,

Kubiak³

2014

Polish Journal of Veterinary Sciences

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The aim of this study was to investigate the levels of circulating immune complexes (CICs) and concentration of haptoglobin, fibrinogen and C-reactive protein in the serum of horses with recurrent airway obstruction and healthy controls. The study was conducted on a group of 14 adult Polish Konik horses, kept in uniform environmental and living conditions. Horses were divided into two groups: 7 horses were not affected by any respiratory problem (control group) and 7 horses had a history of recurrent airway obstruction (RAO) (study group). A clinical and laboratory evaluation, endoscopic examination and bronchoalveolar lavage (BAL) were performed in all horses. Levels of circulating immune complexes were significantly (p=0.0057) increased in heaves-affected horses compared to healthy controls (median [25 th -75 th percentiles]) (3.96 [3.96 -4.43] vs. 7.46 [5.13 -11.9]). No significant difference was observed in the levels of the examined acute phase proteins between the groups. Moreover, all results were within the reference range established for horses. The results of this study indicate that heaves in horses is associated with the formation and high level of CICs. Haptoglobin, fibrinogen and C-reactive protein failed as markers of early stage systemic inflammation in the course of RAO.

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Section: Discussionmentioning

confidence: 99%

Section: Collection and Cytologic Evaluation Of Balfmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Circulating immune complexes and markers of systemic inflammation in RAO-affected horses

Niedźwiedź¹,

Jaworski²,

Kubiak³

2014

Polish Journal of Veterinary Sciences

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“…Inflammatory cell populations in the lung represent a balance between cellular recruitment, via airway epithelial cell and macrophage‐derived chemotactic cytokines, and removal, via apoptosis and phagocyte‐mediated clearance. Lung granulocytes (neutrophils and macrophages) in horses with RAO exhibit altered apoptosis, which together with increased activity of transcription factors such as nuclear factor‐κB (NF‐κB) and activator protein‐1 (AP‐1) might contribute to the maintenance of neutrophilic inflammation in horses treated with glucocorticoids and maintained in an allergenic environment . Whilst no age‐related trends in BALF cytological profiles in horses with IAD or RAO have been reported, there is an age‐associated increase in mRNA expression of IFN‐γ producing lymphocytes in stimulated BAL cells .…”

Section: Late‐onset Asthmamentioning

confidence: 99%

Equine asthma: Integrative biologic relevance of a recently proposed nomenclature

Bond

Léguillette

Richard

et al. 2018

Veterinary Internal Medicne

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The term “equine asthma” has been proposed as a unifying descriptor of inflammatory airway disease (IAD), recurrent airway obstruction (RAO), and summer pasture‐associated obstructive airway disease. Whilst the term will increase comprehensibility for both the lay and scientific communities, its biologic relevance must be compared and contrasted to asthma in human medicine, recognizing the limited availability of peer‐reviewed equine‐derived data, which are largely restricted to clinical signs, measures of airway obstruction and inflammation and response to therapy. Such limitations constrain meaningful comparisons with human asthma phenotypes. Suggested minimum inclusion criteria supporting the term asthma, as well as similarities and differences between IAD, RAO, and multiple human asthma phenotypes are discussed. Furthermore, differences between phenotype and severity are described, and typical features for equine asthma subcategories are proposed. Based on shared features, we conclude that mild/moderate (IAD) and severe (RAO) equine asthma are biologically appropriate models for both allergic and non‐allergic human asthma, with RAO (severe equine asthma) also being an appropriate model for late‐onset asthma. With the development of new biologic treatments in humans and the application of more targeted therapeutic approaches in the horse, it would appear appropriate to further investigate the allergic (Th‐2) and non‐allergic (non‐Th‐2) phenotypes of equine asthma. Further research is required to more fully determine the potential clinical utility of phenotype classification.

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“…Only one study reported an increased expression of apoptotic related proteins in adipose and hepatic tissue of horses with equine metabolic syndrome [64]. However, other reports have studied the effects of different diseases, such as asthma [65], colitis and lipopolysaccharide infusion [66,67], upon neutrophil apoptosis. Interestingly, delayed neutrophil apoptotic rates have been reported in the studies mentioned above.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Peripheral Blood Neutrophil Functions after an Oral Carbohydrate Overload in Obese and Insulin Resistant Horses

Salinas¹,

Espinosa²,

Morales³

et al. 2020

Preprint

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Background: Obesity and insulin resistance (IR) are conditions of increasing prevalence in populations of equids worldwide. The immune impairment described in metabolic dysfunction status in humans has been extensively reported with minimal data regarding horses. The objective of the study was to evaluate the effect of obesity as an isolated factor and in association with insulin resistance on apoptosis, phagocytosis and oxidative burst activity of neutrophils isolated from peripheral blood of lean and obese adult horses with or without insulin resistance, basally and after induction of hyperglycemia through an oral glucose test. Results: No differences in apoptosis were observed between experimental groups at any time point. Phagocytic capacity was significantly diminished at baseline in the obese-IR group (P<0.05) but increased after stimulation of hyperglycemia (P=0.007). Basal reactive oxygen species production differed significantly (P=0.0001) between the obese-insulin sensitive (IS) and lean-IS or obese-IR groups, and decreased significantly after stimulation of hyperglycemia in the lean-IS and obese-IS groups (P<0.05).Conclusions: Results from this study showed that both metabolic status itself, and acute hyperglycemia, are factors that influence PMNs functionality in horses, specifically phagocytosis and oxidative burst. This indicates the need for close monitoring of immune function in horses with inflammatory disease and concurrent obesity and insulin resistance.

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Neutrophil and macrophage apoptosis in bronchoalveolar lavage fluid from healthy horses and horses with recurrent airway obstruction (RAO)

Cited by 28 publications

References 31 publications

Circulating immune complexes and markers of systemic inflammation in RAO-affected horses

Circulating immune complexes and markers of systemic inflammation in RAO-affected horses

Equine asthma: Integrative biologic relevance of a recently proposed nomenclature

Evaluation of Peripheral Blood Neutrophil Functions after an Oral Carbohydrate Overload in Obese and Insulin Resistant Horses

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