Neutrophil apoptosis during the resolution of bovine mammary gland injury

Sládek, Z.; Rysanek, D.

doi:10.1053/rvsc.2000.0440

Cited by 34 publications

(37 citation statements)

References 20 publications

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“…The resolution of mammary gland injury was more rapid after PBS treatment than after LPS treatment. This result is consistent with our earlier findings [23,24].…”

Section: Discussionsupporting

confidence: 94%

“…This decrease became apparent at 48 h in PBS-induced and at 72 h in LPS-induced mammary gland injury. These changes closely correlate with the clearance of apoptotic neutrophils, as determined in our previous paper in which the highest counts of apoptotic neutrophils and myeloperoxidase-positive mononuclear phagocytes were found at 48 and 72 h in PBS-induced and LPS-induced mammary gland injury, respectively [23,24]. This difference can be attributed to LPS which prolonged the influx of neutrophils and delayed the onset of neutrophil apoptosis.…”

Section: Discussionsupporting

confidence: 84%

“…Intensive phagocytosis of apoptotic neutrophils at 48 h after mammary gland injury induction, confirmed by staining for myeloperoxidase, was demonstrated in our previous studies [23,24]. Between 48 and 72 h, the percentage of myeloperoxidasepositive monocytes in the cavity system of the mammary gland exceeded 40 and morphologically distinct, recently phagocytised neutrophils were observed in 1/4 of them.…”

Section: Discussionsupporting

confidence: 63%

“…Influx of leukocytes can also be induced by intramammary treatment with PBS [4,20]. The influx in which neutrophil leukocytes predominate, culminates within 24 h. Mammary gland injury is resolved after 72 to 96 h and the major role is played by apoptosis and phagocytosis of apoptotic neutrophils by mononuclear phagocytes [23,24]. The elimination of neutrophils is associated with shifts in differential counts of other cell types.…”

Section: Discussionmentioning

confidence: 99%

“…The resolution of LPS-induced mammary gland inflammation is characterised by significant interactions among inflammatory cells which result in the subsidence of influx by apoptosis of neutrophils. Mononuclear phagocytes of the cavity system of the mammary gland participates in the clearance of apoptotic neutrophils by phagocytosis [23,24]. Mononuclear phagocytes of heifers mammary glands include two morphologically distinct cell types.…”

Section: Introductionmentioning

confidence: 99%

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Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Sládek¹,

Rysanek²,

Faldyna

2002

Vet. Res.

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-The object of the study was the comparative assessment of phagocyte activation during initiation and resolution of mammary gland injury induced by lipopolysaccharide (LPS) or buffered salt solution (PBS) on the basis of the CD14 receptor positivity. The experiments were carried out in 15 clinically normal Holstein × Bohemian Red Pied crossbred heifers, aged 14 to 18 months. Noninflammatory and inflammatory mammary gland injury were induced by intramammary administration of PBS (10 mL) and LPS (10 mL, 1 µg/mL), respectively. Samples of the cell populations were obtained by mammary lavages at 24 h intervals. Flow cytometry was used to determine the CD14+ neutrophils, monocytes, and macrophages. The percentage of CD14+ neutrophils was only 1.2% and 1.3% 24 h after the treatment with PBS and LPS, respectively. The resolution was accompanied by an increase in proportion of CD14+ neutrophils. The proportion of CD14+ neutrophils returned to initial values in the PBS-treated, but not in the LPS-treated mammary glands till 96 h. Percentage of CD14+ monocytes increased after 24 h and the effect was more pronounced in the LPS-treated than in the PBS treated mammary glands (P < 0.05). The percentage of CD14+ macrophages decreased highly significantly at 24 h in the LPS-treated, but not in the PBS-treated mammary glands (P < 0.01). The resolution of mammary gland injury (48 to 96 h) was characterised by an increase in CD14+ macrophages proportion, which was greater in the LPS-treated than PBS-treated mammary glands (P < 0.01). The activation of macrophages during resolution of mammary gland injury can be interpreted as an important mechanism of restitution. Résumé -Activation des phagocytes au cours de l'initiation et de la résolution de lésion de la glande mammaire induite par des lipopolysaccharides chez des génisses. L'objet de la présente étude est l'analyse de l'activation des phagocytes au cours de l'initiation et de la résolution de lésion de la glande mammaire induite par des lipopolysaccharides (LPS), ou par la solution physiologique (PBS), et cela sur la base de la positivité du CD14 récepteur. L'essai fut réalisé sur 15 génisses, cliniquement en bonne santé, issues du croisement des races Holstein × Pie rouge tchèque, âgées de 14 à 18 mois. Des lésions non-inflammatoires et inflammatoires de la glande mammaire ont été induites par l'administration intra-mammaire de PBS (10 mL) et LPS (10 mL, 1 µg/mL), respectivement. Les échantillons de populations cellulaires ont été obtenus par lavage des glandes mammaires, à des intervalles de 24 heures. On a utilisé la cytométrie de flux pour détecter les neutrophiles, les monocytes et les macrophages CD14+. Pendant l'induction de la lésion de glande mammaire (24 h), on a détecté seulement 1,2 % des neutrophiles CD14+ après traitement par le PBS et 1,3 % après traitement par le LPS. La résolution fut caractérisée par une augmentation de la proportion de neutrophiles CD14+. Jusqu'à 96 heures, après injection de PBS, à la différence de celle de LPS, la proportion de ne...

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“…The resolution of mammary gland injury was more rapid after PBS treatment than after LPS treatment. This result is consistent with our earlier findings [23,24].…”

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Sládek¹,

Rysanek²,

Faldyna

2002

Vet. Res.

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Physiological and Immunological Tools and Techniques for the Assessment of Cattle Welfare

Earley,

Buckham-Sporer,

O’Loughlin

et al. 2023

Animal Welfare

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Bovine neutrophils in health and disease

Bassel

Caswell

2018

Cell Tissue Res

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Bovine neutrophils have similarities to those of other species with respect to mechanisms of their activation and migration into tissue, modulation of immune responses and the balance between microbial killing and host tissue damage. However, bovine neutrophils have biochemical and functional differences from those of other species, which may yield insights about the comparative biology of neutrophils. Neutrophils play protective and harmful roles in the infectious diseases of cattle that occur at times of transition: respiratory disease in beef calves recently arrived to feedlots and mastitis and other diseases of postparturient dairy cows. An important research focus is the mechanisms by which risk factors for these diseases affect neutrophil function and thereby lead to disease and the prospect of genetic or pharmacologic improvement of disease resistance. Further, in keeping with the One Health paradigm, cattle can be considered a model for studying the role of neutrophils in naturally occurring diseases caused by host-adapted pathogens and are thus an intermediary between studies of mouse models and investigations of human disease. Finally, the study of bovine neutrophils is important for agriculture, to understand the pathogenesis of these production-limiting diseases and to develop novel methods of disease prevention that improve animal health and reduce the reliance on antimicrobial use.

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Neutrophil apoptosis during the resolution of bovine mammary gland injury

Cited by 34 publications

References 20 publications

Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Physiological and Immunological Tools and Techniques for the Assessment of Cattle Welfare

Bovine neutrophils in health and disease

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