Neutrophil CD11b and soluble ICAM-1 and E-selectin in community acquired pneumonia

Glynn, P.; Coakley, R.; Kilgallen, I.; O'Neill, S.

doi:10.1183/09031936.99.13613869

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…First, increase in CD11b expression level is a specific marker as no increase was observed in normal subjects. This finding are in agreement with data previously reported by Wang et al (1998) and Glynn et al (1999) who showed an up-regulation of neutrophil CD11b expression in community-acquired pneumonia and respiratory syncytial virus (RSV)-induced bronchiolitis, respectively. Moreover, an increased of CD11b was observed in two infected patients who presented with pneumonia without septicemia (Table 1, patients 6 and 9).…”

Section: Clinical Applicationsupporting

confidence: 93%

A standardized procedure for quantitation of CD11b on polymorphonuclear neutrophil by flow cytometry: potential application in infectious diseases

Latger‐Cannard

Besson²,

Doco‐Lecompte

et al. 2004

Clin Lab Haematol

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An up-regulation of the surface marker CD11b has been demonstrated during polymorphonuclear (PMN) cell activation. CD11b over-expression is often associated with inflammation and is considered as an early marker of infection. However, the absence of standardized assay and the variability of preanalytical settings leading to PMN artifactual activation have compromised the interest of this marker. In the present study a standardized quantitative flow cytometry assay directly performed in whole blood has been used to determine CD11b expression on PMN cells. The results indicate that quantitative flow cytometry can provide consistent CD11b density values between laboratories provided that a calibration system is used including specific calibrators, reagents and protocols. This method allowed us to evidence an up-regulation of CD11b expression for infected patients. This quantitation is a standardized and potentially useful method in clinical situations implying quantitative CD11b expression variations.

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Section: Clinical Applicationsupporting

confidence: 93%

A standardized procedure for quantitation of CD11b on polymorphonuclear neutrophil by flow cytometry: potential application in infectious diseases

Latger‐Cannard

Besson²,

Doco‐Lecompte

et al. 2004

Clin Lab Haematol

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“…In ALI/ARDS, the inflamed lesion spreads over the original pneumonic lung segment, leading to the accumulation of leukocytes in a large pulmonary area. sES is selectively expressed in vascular endothelial cells, and plays important roles in the accumulation of leukocytes during pneumonia 26 . Thus, elevation of sES in pneumonia patients is thought to indicate the presence of major pulmonary parenchymal inflammation.…”

Section: Discussionmentioning

confidence: 99%

Soluble Endothelial Selectin in Acute Lung Injury Complicated by Severe Pneumonia

Osaka¹,

Shibata²,

Kanouchi³

et al. 2011

Int. J. Med. Sci.

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Background: Pneumonia is still one of the most frequent causes of death in the elderly. Complication of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) by pneumonia makes patients very ill due to severe respiratory failure. Biomarkers that can discriminate the presence of complicating ALI/ARDS are required for early detection. The aim of this research was to investigate whether soluble endothelial selectin (sES) could be a biomarker for ALI.Methods: Serum sES levels were measured in 27 pneumonia patients, who were enrolled between April 2006 and September 2007. Among these patients, six had ALI or a condition that was clinically comparable to ALI (cALI). All patients who were enrolled were successfully treated and survived.Results: Circulating sES levels were elevated in pneumonia patients with ALI/cALI, and sES levels decreased following treatment of their pneumonia. Univariate and multivariate logistic regression analyses showed that sES was the only significant factor for identifying complicating ALI/cALI, independently of C-reactive protein (CRP) and lactate dehydrogenase (LDH). By receiver operating characteristic (ROC) curve analysis, the cut-off value for sES was 40.1 ng/mL, with a sensitivity of 0.8 and a specificity of 0.8.Conclusion: sES may be a useful biomarker for discriminating complicating ALI/cALI in patients with severe pneumonia.

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“…It has indeed been reported that systemic inflammatory status increases with age (Franceschi and Campisi ), but several studies also reported little or no difference in the level of expression of individual leukocytes surface markers, including CD11b, between elderly and young subjects (Glynn et al. ; Noble et al. ).…”

Section: Discussionmentioning

confidence: 99%

Effects of intravenous glucose and lipids on innate immune cell activation in healthy, obese, and type 2 diabetic subjects

et al. 2015

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Atherosclerosis/cardiovascular disease are major causes of morbidity/mortality in obesity and type 2 diabetes (T2D), and have been associated with activation of innate immune cells, their diapedesis to the arterial intima and formation of the atherosclerotic plaque. While in obesity/T2D immune cell activation likely depends on dysregulated metabolism, the interaction between individual metabolic factors typical of these conditions (hyperglycemia, hyperlipidemia), innate immune cell activation, and the progression of atherosclerosis remains unclear. We, therefore, measured by flow cytometry cell surface expression of CD11b, CD14, CD16, CD62L, and CD66b, known markers of granulocyte (Gc) and monocyte (Mc) activation, in five healthy, five obese, and five T2D subjects, during 4-h i.v. infusions of 20% dextrose (raising blood sugar levels to ∼220 mg/dL), 20% Intralipid (raising trygliceride levels to ∼6 mmol/L), or a combination of the two. We hypothesized that both glucose and lipids would increase Gc/Mc surface marker expression, and simultaneous infusion would have an additive or synergistic effect. Surprisingly, though, infusion of glucose alone had little effect, while lipids, alone or combined with glucose, significantly increased expression of several markers (such as CD11b in Gc and Mc, and CD66 b in GC) within 60–90 min. Less pronounced increases in systemic inflammatory cytokines also occurred in obese and T2D subject, with no acute changes in gene expression of the the proinflammatory genes NFκB and CCR2. Our results suggest that lipids may be stronger acute contributors to innate cell activation than acute hyperglycemia per se, possibly helping shape more effective preventive dietary guidelines in T2D.

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Neutrophil CD11b and soluble ICAM-1 and E-selectin in community acquired pneumonia

Cited by 4 publications

References 20 publications

A standardized procedure for quantitation of CD11b on polymorphonuclear neutrophil by flow cytometry: potential application in infectious diseases

A standardized procedure for quantitation of CD11b on polymorphonuclear neutrophil by flow cytometry: potential application in infectious diseases

Soluble Endothelial Selectin in Acute Lung Injury Complicated by Severe Pneumonia

Effects of intravenous glucose and lipids on innate immune cell activation in healthy, obese, and type 2 diabetic subjects

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