Neutrophil CD64 expression is associated with severity and prognosis of disseminated intravascular coagulation

Song, Sang Hoon; Kim, Hyun Kyung; Park, Myoung Hee; Cho, Han‐Ik

doi:10.1016/j.thromres.2007.05.013

Cited by 34 publications

(29 citation statements)

References 27 publications

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“…However, it is also increased in cases of SIRS due to non-infectious disease conditions such as severe congestive heart failure or acute pancreatitis, and viral and parasitic infections [30,31]. The accuracy of CD64 index to detect positive sepsis in subjects with VAP was superior compared to other parameters, which was actually in accordance with other studies [32,33]. We also found that in subjects with severe disease, such as VAP-induced sepsis, slightly higher cut-off values for positivity in CD64 index (1.58), CRP (163.5 mg/L), and PCT (1.73 µg/L) levels are required to obtain the best sensitivity, specificity, and predictive values.…”

Section: Discussionsupporting

confidence: 86%

“…However, we found that a CD64 index cut-off of 1.58 may be optimal for prediction of survival length in subjects with VAP-induced sepsis. The levels were significant (p = 0.046), and survival curve analysis showed much longer time of survival in subjects with lower CD64 index, similar to the findings of Song et al [33] and Muller et al [38]. In addition, the culturing methods are also of great importance because they identify the pathogen, and more focused and targeted antibiotic therapy could be applied, which could prolong the survival of the subject or even cure the disease.…”

Section: Discussionsupporting

confidence: 80%

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CD64 index on neutrophils can diagnose sepsis and predict 30-day survival in subjects after ventilator-associated pneumonia

Muzlovič

Ihan

Stubljar

2016

J Infect Dev Ctries

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Introduction: Sepsis associated with ventilator-associated pneumonia (VAP) causes mortality in intensive care unit (ICU) patients. The time of diagnosis is crucial, and microbiological cultures take time. In this study, the diagnostic accuracy of CD64 index to predict VAP-induced sepsis and survival time in subjects requiring mechanical ventilation were evaluated and compared to conventional biomarkers and culturing methods. Methodology: A total of 32 subjects with VAP were included. Sepsis after VAP was diagnosed in 25 (78.1%) patients according to clinical signs, radiographic examination, and samples of blood and trachea taken for culturing. Simultaneously with cultures, CD64 index on neutrophils, C-reactive protein (CRP), procalcitonin (PCT), and count of leucocytes and neutrophils were determined. Results: Biomarker values were evaluated in both groups of subjects (with and without sepsis after VAP). The values of CD64 index and CRP were significantly higher in the sepsis group. Receiver operating characteristic (ROC) curve analysis revealed an area under curve (AUC) of 0.929 for CD64 index in differentiating subjects with VAP-induced sepsis from those without sepsis. The biomarkers CRP and PCT showed comparable results (AUC of 0.869 and 0.909, respectively). Blood cultures were positive in 12 subjects, endotracheal aspirate in 19. CD64 index and isolation of pathogen with positive blood cultures or from endotracheal aspirate (positive in 24 cases) could predict survival time before application of more targeted antibiotic therapy. Conclusions: CD64 index may be used as a useful diagnostic tool to recognize VAP-induced sepsis; moreover, accompanied with an identified pathogen, can predict survival for ICU patients.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 80%

CD64 index on neutrophils can diagnose sepsis and predict 30-day survival in subjects after ventilator-associated pneumonia

Muzlovič

Ihan

Stubljar

2016

J Infect Dev Ctries

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“…Livaditi et al showed that neutrophil CD64 expression and serum IL-8 were sensitivity early markers of severity and 28-day mortality in patients with sepsis [27] [24]. In that study, the median (range) CD64 values for patients who died compared with those who survived were [26]. In the present study, we found that patients who survived showed a higher CD64 index when compared with patients who died.…”

Section: Discussionsupporting

confidence: 59%

“…Moreover, we decided to exclude children because we wanted to focus on adult patients to have a homogeneous group of patients and to avoid a possible source of variation. Second, we did not use healthy people as controls because it is well known that the apparent accuracy of a test is likely to be misleading high [25,26] [22,23].…”

Section: Discussionmentioning

confidence: 99%

Neutrophil CD64: diagnostic accuracy and prognostic value in patients presenting to the emergency department

Cid

García‐Pardo

Aguinaco

et al. 2011

Eur J Clin Microbiol Infect Dis

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Purpose: To evaluate the diagnostic accuracy and prognostic value of neutrophil CD64 expression for bacterial infection in febrile adult patients presenting to our hospital emergency department. Methods:We prospectively included 132 patients with with fever ≥38º C (≥100.4º F) during the last 24 hours and we did the measurement of CD64 on neutrophils the day after the admission of the patient at the emergency department. We followed the patients until the full recovery or death.

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“…Our study corroborates various FCM methods which can predict prognosis in sepsis [25][26][27] increased mortality in adults is being associated with upregulation of nCD64 [28,29] while downregulation showed for non-survivors [30]; however, in sepsis, nCD64 showed a prognostic significance [25]. In another study, Ng et al demonstrated no significant difference between infected and non-infected or control groups for mHLA-DR expression [31].…”

Section: Chauhan and Hansasupporting

confidence: 86%

Early Diagnosis of Sepsis Through Sepsis Markers and Sepsis Index Through Flow Cytometry Technology

Chouhan

Hansa

Pradhan³

2017

Asian J Pharm Clin Res

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Objective: Sepsis is a major medical challenge, and remains a burden by showing minimal symptoms, non-specific manifestation which leads to the high-mortality rate. The major cause of high mortality rate is due to the diagnostic pitfalls. The sepsis mechanism involves immune suppression associated with multiorgan dysfunction, uncontrolled infection, and death. This study aims to use new parameters in diagnosis of sepsis particularly neutrophil CD64 (nCD64), monocyte human leukocyte antigen-DR (mHLA-DR), performed by flow cytometer. Methods:Available diagnostic tools such as blood culture (gold standard) and other tools are time-consuming which leads to death because the treatment is not commenced promptly due to unavailability of quick and accurate diagnostic procedures. To overcome such instances, there are different specific cell surface markers which are introduced to reveal sepsis at the earliest using flow cytometry technique. The newer technique allows determination of different cellular and functional pathological components of sepsis. Using this technology, a newer treatment modality (granulocyte macrophage colony-stimulating factor) can be used to reverse sepsis associated immune suppression. Results:The results suggested that the flow cytometric evaluation of nCD64, mHLA-DR expression assay, seems to be promising as comparison to other tools available for diagnosis. Conclusion:The pilot data suggest that the flow assay, "sepsis index" is a useful assay for diagnosing and discriminating sepsis in adult at intensive care unit settings in comparison to all the available current modalities of sepsis diagnosis. However, there are no other comparable parameters to diagnose early sepsis.

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Neutrophil CD64 expression is associated with severity and prognosis of disseminated intravascular coagulation

Cited by 34 publications

References 27 publications

CD64 index on neutrophils can diagnose sepsis and predict 30-day survival in subjects after ventilator-associated pneumonia

CD64 index on neutrophils can diagnose sepsis and predict 30-day survival in subjects after ventilator-associated pneumonia

Neutrophil CD64: diagnostic accuracy and prognostic value in patients presenting to the emergency department

Early Diagnosis of Sepsis Through Sepsis Markers and Sepsis Index Through Flow Cytometry Technology

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