Neutrophil Extracellular Traps in Ulcerative Colitis

Bennike, Tue Bjerg; Carlsen, Thomas Gelsing; Ellingsen, Torkell; Bonderup, Ole K.; Glerup, Henning; Bøgsted, Martin; Christiansen, Gunna; Birkelund, Svend; Stensballe, Allan; Andersen, Vibeke

doi:10.1097/mib.0000000000000460

Cited by 146 publications

(144 citation statements)

References 76 publications

Supporting

Mentioning

135

Contrasting

Unclassified

Order By: Relevance

“…This mechanism is very likely to operate in the colorectal mucus massively infiltrated with granulocytes and may at least partially explain the recently reported presence of proteins of extracellular traps in biopsy samples from patients with UC (30).…”

Section: Discussionmentioning

confidence: 95%

Colorectal mucus non‐invasively collected from patients with inflammatory bowel disease and its suitability for diagnostic cytology

Bandaletova

Chhaya

Poullis

et al. 2015

APMIS

View full text Add to dashboard Cite

Colorectal mucus is a key component of the protective gut barrier which is altered in inflammatory bowel disease (IBD). We aimed to cytologically characterize colorectal mucus non-invasively collected from IBD patients using our new sampling technique. Colorectal mucus was self-collected by 58 IBD patients comprising 31 ulcerative colitis (UC) and 27 Crohn's disease (CD) cases. The samples were examined cytologically, and immunocytochemically. Large numbers of well-preserved granulocytes were typically detected (neutrophils undergoing degradation were observed as well). Plasma cells and erythrophagocytosis were present in 18.2% and 29.1% of cases, respectively, predominantly in patients with UC and distal CD. Immunocytochemical visualization of calprotectin in neutrophils, eosinophil-derived neurotoxin in eosinophils and tumour necrosis factor-α in macrophages was also achieved. Correct cytological diagnosis was made in 61.8% of analysed IBD cases. Our new method of colorectal mucus sampling provides highly informative material for cytology. Findings of the presence of plasmocytes and erythrophagocytosis in colorectal mucus are unique and may reflect previously unknown mechanisms of IBD pathogenesis. Immunocytochemical detection of inflammation biomarkers demonstrates the suitability of this material for biomarker quantification. These promising results suggest a potential role for colorectal mucus cytology in the non-invasive diagnosis of IBD.

show abstract

Section: Discussionmentioning

confidence: 95%

Colorectal mucus non‐invasively collected from patients with inflammatory bowel disease and its suitability for diagnostic cytology

Bandaletova

Chhaya

Poullis

et al. 2015

APMIS

View full text Add to dashboard Cite

show abstract

“…Presence and activity of NETs have been recently identified in non-infectious inflammatory diarrhea e.g. ulcerative colitis [74] as well. Appearance of NETs have been strongly associated with ROS formation [72] and with strong evidence of ROS formation and overall neutrophil activation in response to C. difficile , we speculate that NETs may contribute to clearance of C. difficile bacterium as well.…”

Section: Neutrophil-mediated Inflammation: Friend or Foe?mentioning

confidence: 99%

Neutrophil-mediated inflammation in the pathogenesis of Clostridium difficile infections

Jose

Madan

2016

Anaerobe

View full text Add to dashboard Cite

Clostridium difficile is the most important cause of nosocomial infectious diarrhea in the western world. C. difficile infections are a major healthcare burden with approximately 500,000 new cases every year and an estimated annual cost of nearly $1 billion in the U.S. Furthermore, the infections are no longer restricted to health care facilities, and recent studies indicate spread of C. difficile infection to the community as well. The clinical spectrum of C. difficile infection ranges from asymptomatic colonization to severe diarrhea, fulminant colitis and death. This spectrum results from a complex interplay between bacterial virulence factors, the colonic microbiome and the host inflammatory response. The overall vigor of host inflammatory response is believed to be an important determinant of C. difficile disease severity, and a more robust immune response is associated with worse outcomes. Neutrophils are the primary cells that respond to C. difficile invasion and neutrophilic inflammation is the hallmark of C. difficile-associated disease. In this review, we will focus on the role of neutrophils (infiltration to infected tissue, pathogen clearance and resolution of inflammation) in the immuno-pathogenesis of C. difficile-associated disease (CDAD).

show abstract

“…Briefly, plasma samples were denatured, reduced, alkylated, filtered by YM-10 kDa spinfilter (Millipore) and digested overnight prior to analysis by UPLC-MS using a nano-UPLC system coupled online to a Q-Exactive mass HF spectrometer (Thermo Scientific). 29 Peptide signaling intensities from full MS scans were imported and further processed in MaxQuant 1.5.2.6 software and Perseus software (http://www.coxdocs.org/doku.php?id¼:per seus:start). 30 Protein identification was performed by data searching against Uniprot Sus Scrofa protein reference proteome databases and an in-house optimized PeptideAtlas database for Sus scrofa.…”

Section: Plasma Proteomics For Preterm Pigs With Spontaneous Developmmentioning

confidence: 99%

Elevated levels of circulating cell-free DNA and neutrophil proteins are associated with neonatal sepsis and necrotizing enterocolitis in immature mice, pigs and infants

et al. 2017

Self Cite

View full text Add to dashboard Cite

Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC), but disease pathogenesis and specific diagnostic markers are lacking. Circulating cell-free DNA (cfDNA) and immune cell-derived proteins are involved in multiple immune diseases in adults but have not been investigated in preterm neonates. We explored the relation of circulating neutrophil-associated proteins and cfDNA to LOS and/or NEC. Using a clinically relevant preterm pig model of spontaneous LOS and NEC development, we investigated neutrophil-associated proteins and cfDNA in plasma, together with cytokines in gut tissues. The changes in cfDNA levels were further studied in preterm pigs and neonatal mice with induced sepsis, and in preterm infants with or without LOS and/or NEC. Fifteen of 114 preterm pigs spontaneously developed both LOS and NEC, and they showed increased intestinal levels of IL-6 and IL-1b and plasma levels of cfDNA, neutrophil-associated proteins, and proteins involved in platelet-neutrophil interaction during systemic inflammation. The abundance of neutrophil-associated proteins highly correlated with cfDNA levels. Further, Staphylococcus epidermidis challenge of neonatal mice and preterm pigs increased plasma cfDNA levels and bacterial accumulation in the spleen. In infants, plasma cfDNA levels were elevated at LOS diagnosis and 1-6 d before NEC. In conclusion, elevated levels of plasma cfDNA and neutrophil proteins are associated with LOS and NEC diagnosis.

show abstract

Neutrophil Extracellular Traps in Ulcerative Colitis

Abstract: Article first published online 19 May 2015.

Cited by 146 publications

References 76 publications

Colorectal mucus non‐invasively collected from patients with inflammatory bowel disease and its suitability for diagnostic cytology

Colorectal mucus non‐invasively collected from patients with inflammatory bowel disease and its suitability for diagnostic cytology

Neutrophil-mediated inflammation in the pathogenesis of Clostridium difficile infections

Elevated levels of circulating cell-free DNA and neutrophil proteins are associated with neonatal sepsis and necrotizing enterocolitis in immature mice, pigs and infants

Contact Info

Product

Resources

About