Neutrophil Extracellular Traps Induced in Rheumatoid Arthritis Conditioned Animals are Inhibited Through Selenium Nanoparticles Supplementation

Abstract

Growing experimental evidence shows that the neutrophil extracellular traps (NETs) plays vital contribution in rheumatoid arthritis (RA). Selenium (Se) and Se nanoparticles (SeNPs) known to modulate RA-induced pathogenesis through antioxidant gene modulation. In the present study we have inferred that SeNPs supplementation effectively controls NETs formation, which in turn could curtail RA-induced inflammatory response.Neutrophils obtained from different experimental conditions were used to evaluate the in vitro NETs formation and inhibition of through SeNPs supplementation. Increased oxidative stress, decreased antioxidant enzyme activities and increased inflammatory cytokines were observed in neutrophils of RA, whereas SeNPs treatment attenuate it. Neutrophils obtained from control and SeNPs supplemented groups do not have statistically significant Se level between the groups, on the other hand reduced the oxidative stress. Neutrophils of RA forms more spontaneous NETs in vitro culture than that of control and SeNPs treated neutrophils. Neutrophils obtained from RA rats are more inclined for external NETs inducing agent such as lipopolysaccharides and phorbol 12-myristate 13-acetate, when compared with SeNPs treated and control neutrophils. On the other hand in vitro pre-treatment of neutrophils with SeNPs before exposing to NETs inducing substances, indicate the anti-NETs forming property of SeNPs. This effect could be mediated through reduction in major inflammatory mediators namely TNF-α, IL-17 and IL-6. This findings confirms that SeNPs could act as effective NETs formation blocking agent. Our present and previous observation conclude that SeNPs, could serve as an effective anti-arthritic agent warranting human study. Furthermore, this study also throws light on the new information such as SeNPs which could be used as therapeutics agent, where NETs is major pathogenic factor.

Biogenic Selenium Nanoparticles: Potential Solution to Oxidative Stress Mediated Inflammation in Rheumatoid Arthritis and Associated Complications

“…SeNPs reduced the NETs as exhibited in SeNP treatment RA mice models. The neutrophils in RA have shown increased levels of OS, lowered action of antioxidant enzymes, and more inflammation related cytokines, and treatment of SeNPs attenuated these abnormal levels as a possible mechanism of decreased NETs [92]. Foeniculum vulgare Mill.-derived SeNPs exhibited antioxidant and anti-inflammation potential in arthritic albino, laboratory-bred strain of the house mouse (BALB/c) mice models; SeNPs at a dosage of 10 mg/kg showed significant potential in a 2,2-Diphenyl-1-Picrylhydrazyl (DPPH)test, reduction in paw volume, and reversal in biochemical parameters in a treated BALB/c group [93].…”