Neutrophil Function Assays

Ladero-Auñon, Iraia; Elguezabal, Natalia

doi:10.1016/b978-0-12-818731-9.00130-0

Cited by 2 publications

(2 citation statements)

References 60 publications

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“…Neutrophils kill microbes through a set of mechanisms that include phagocytosis, reactive oxygen species (ROS) production, degranulation, and extracellular trap (ET) release [93]. Neutrophils isolated from healthy cattle have shown ET liberation against MAP and the effective killing of these bacteria in vitro [94].…”

Section: Neutrophilsmentioning

confidence: 99%

“…Neutrophils are tricky cell types to work with, and functional assays must be performed with freshly isolated cells, making this model less attractive than monocytes or macrophages. The number of protocols for the isolation, culture, and evaluation of functionality is continuously growing, and are largely dependent on the host species [93]. An improvement of the methodology will probably nurture further research on this phagocytic cell and its role in PTB.…”

Section: Neutrophilsmentioning

confidence: 99%

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Modeling Paratuberculosis in Laboratory Animals, Cells, or Tissues: A Focus on Their Applications for Pathogenesis, Diagnosis, Vaccines, and Therapy Studies

Jolly,

Fernández,

Mundo

et al. 2023

Animals

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Paratuberculosis is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. Paratuberculosis that affects a wide variety of domestic and wild animals. It is considered one of the diseases with the highest economic impact on the ruminant industry. Despite many efforts and intensive research, paratuberculosis control still remains controversial, and the existing diagnostic and immunoprophylactic tools have great limitations. Thus, models play a crucial role in understanding the pathogenesis of infection and disease, and in testing novel vaccine candidates. Ruminant animal models can be restricted by several reasons, related to space requirements, the cost of the animals, and the maintenance of the facilities. Therefore, we review the potential and limitations of the different experimental approaches currently used in paratuberculosis research, focusing on laboratory animals and cell-based models. The aim of this review is to offer a vision of the models that have been used, and what has been achieved or discovered with each one, so that the reader can choose the best model to answer their scientific questions and prove their hypotheses. Also, we bring forward new approaches that we consider worth exploring in the near future.

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Section: Neutrophilsmentioning

confidence: 99%

Section: Neutrophilsmentioning

confidence: 99%

Modeling Paratuberculosis in Laboratory Animals, Cells, or Tissues: A Focus on Their Applications for Pathogenesis, Diagnosis, Vaccines, and Therapy Studies

Jolly,

Fernández,

Mundo

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

Modeling Paratuberculosis in Laboratory Animals, Cells or Tissues: Focus on Their Applications for Pathogenesis, Diagnosis, Vaccines and Therapies Studies

Jolly,

Fernández,

Mundo

et al. 2023

Preprint

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Paratuberculosis (PTB) is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP) that affects a wide variety of domestic and wild animals. It is considered as one of the diseases with the highest economic impact in the ruminant industry. Despite many efforts and intensive research, PTB control is still controversially discussed and diagnostic and immunoprophylactic tools lack great limitations. Thus, models play a crucial role in understanding the pathogenesis of infection and disease, and in testing novel vaccine candidates. Here, we review the potential and limitations of different experimental approaches currently used in PTB research, focusing on laboratory animals and cell based models. The aim of this review is to offer a vision of the models that have been used and what has been achieved or discovered with each one so that the reader can choose the best model to answer their scientific questions and prove their hypotheses. Also, we bring forward new approaches that we consider worth exploring in the near future.

show abstract

Neutrophil Function Assays

Cited by 2 publications

References 60 publications

Modeling Paratuberculosis in Laboratory Animals, Cells, or Tissues: A Focus on Their Applications for Pathogenesis, Diagnosis, Vaccines, and Therapy Studies

Modeling Paratuberculosis in Laboratory Animals, Cells, or Tissues: A Focus on Their Applications for Pathogenesis, Diagnosis, Vaccines, and Therapy Studies

Modeling Paratuberculosis in Laboratory Animals, Cells or Tissues: Focus on Their Applications for Pathogenesis, Diagnosis, Vaccines and Therapies Studies

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