Neutrophil gelatinase-associated lipocalin: A new antioxidant that exerts its cytoprotective effect independent on Heme Oxygenase-1

Abstract

Neutrophil Gelatinase-Associated Lipocalin (NGAL/Lcn2), a member of the lipocalin family, has a variety of functions. There are extensive studies examining the expression of NGAL under harmful conditions. However, its precise function remains poorly understood. Heme Oxygenase 1 (HO-1) is an enzyme with well-established cytoprotective effects. Previous work showed that NGAL induces expression of HO-1. Interestingly, the same stimuli induced the expression of both NGAL and HO-1. The current study was designed to (1) determine whether NGAL exerts its cytoprotective effect through HO-1 and (2) compare NGAL and HO-1 with each other in terms of their protective role against oxidative stress. The current data indicate that NGAL exerts its cytoprotective effect independent of HO-1 and protects cells against oxidative stress more efficiently than HO-1. The data also strongly suggest that induction of NGAL under harmful conditions is a compensatory response to ameliorate oxidative stress-mediated toxicity. These findings may suggest new applications of NGAL, particularly when oxidative stress is a major factor.

Transcriptome profiling of the newborn mouse lung after hypoxia and reoxygenation: hyperoxic reoxygenation affects mTOR signaling pathway, DNA repair, and JNK-pathway regulation

“…The acutephase glycoprotein LCN2 is active under inflammation, apoptosis, and suppression of bacterial growth. There is recent evidence that Lcn2 also has a pivotal role in the protection against oxidative stress during hypoxia/reoxygenation in vitro (30) and that it acts as an antioxidant (31). Still, we could not detect …”

Diabetes-Induced Renal Injury in Rats Is Attenuated by Suramin

“…It has also been reported that NGAL may play a role in renal adaptation to diabetes, probably as a defensive mechanism to mitigate tubular injury (Bolignano et al, 2009b). NGAL has also been reported to have antioxidant properties (Roudkenar et al, 2007(Roudkenar et al, , 2008(Roudkenar et al, , 2011Bahmani et al, 2010). Of interest, in our study, renal NGAL increased in diabetic rats, and suramin intervention further increased renal NGAL.…”

Atherosclerotic renal artery stenosis is associated with elevated cell cycle arrest markers related to reduced renal blood flow and postcontrast hypoxia

“…Importantly, these markers were higher in patients with reduced RBF and predicted the changes in single-kidney GFR after 3 months of stenting. In a similar pattern, NGAL can be protective against acute ischemic injury, although it is most often utilized as a biomarker for AKI [36]. Our results using BOLD MRI suggest that residual accumulation of deoxyhemoglobin after contrast imaging can persist for at least 24-27 h and extend results observed in experimental rat models that demonstrate renal hypoxia measured by BOLD MRI after injection of iodinated contrast [37].…”