Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period

Manjari, P.; Reddi, Srinu; Alhussien, Mohanned Naif; Mohammed, Seid; De, Sachinandan; Mohanty, A. K.; Jayakumar, S.; Dang, Ajay Kumar

doi:10.1016/j.vetimm.2016.03.017

Cited by 25 publications

(14 citation statements)

References 31 publications

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“…21 In a recent study, we provide in vivo evidence that Day-7 embryo secretes IFNT in the uterus in cows following superovulation and artificial insemination. In consistent with our results, activation of ISGs was also found in peripheral blood leukocytes on Day-8 12 and Day-12 13,22 of early pregnancy in cows. In consistent with our results, activation of ISGs was also found in peripheral blood leukocytes on Day-8 12 and Day-12 13,22 of early pregnancy in cows.…”

Section: F I G U R Esupporting

confidence: 92%

“…6 In the present study, we evaluated gene expression of peripheral blood immune cells at Day-7 of post-inseminations in the same superovulated cows used in our previous study 6 and found that ISGs were up-regulated in both circulating PMNs and PBMCs, suggesting that the circulating immune cells somehow recognize the existence of IFNT in the uterus secreted by Day-7 embryos (compact morulae and blastocysts). In consistent with our results, activation of ISGs was also found in peripheral blood leukocytes on Day-8 12 and Day-12 13,22 of early pregnancy in cows. These findings from ours and others have raised the question how peripheral blood immune cells can recognize IFNT, which is secreted in the uterus locally by the embryos.…”

Section: Progesterone (P4) Concentration In Blood Plasma Was Not Alsupporting

confidence: 92%

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Day‐7 embryos generate an anti‐inflammatory immune response in peripheral blood immune cells in superovulated cows

Talukder

Rashid

Takedomi³

et al. 2018

American J Rep Immunol

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Problem Recently, we demonstrated both in vitro and in vivo that an immunological crosstalk between Day‐7 embryo and immune cells exists locally in the uterus in cows. The peripheral immune response to early embryos at Day‐7 of pregnancy in cows remains largely unknown. Thus, we aimed to investigate the response of peripheral blood immune cells in the presence of multiple Day‐7 embryos in the uterus in donor cows of embryo transfer program. Method of study Superovulated cows were either inseminated (n = 13) at 12‐18 hours post‐estrus or remained without inseminations (n = 6). Blood was collected following insemination (Day‐1) and immediately after uterine flushing (Day‐7). Polymorphonuclear neutrophils (PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated from whole blood for analysis of gene expression. Results Interferon‐stimulated genes (ISGs: ISG15 and OAS1) were increased in both PMNs and PBMCs, with up‐regulation of PTGES and anti‐inflammatory cytokines (TGFB1 and IL10) expressions at Day‐7 of post‐inseminations in cows, when compared to those at Day‐7 in non‐inseminated cows. Conclusion The findings indicate that the presence of multiple embryos in the uterus generates an anti‐inflammatory immune response in peripheral blood immune cells at Day‐7 of pregnancy in cows, which might help to accept semi‐allogenic embryo in the uterus.

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Section: F I G U R Esupporting

confidence: 92%

Section: Progesterone (P4) Concentration In Blood Plasma Was Not Alsupporting

confidence: 92%

Day‐7 embryos generate an anti‐inflammatory immune response in peripheral blood immune cells in superovulated cows

Talukder

Rashid

Takedomi³

et al. 2018

American J Rep Immunol

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“…12 The isolated neutrophil cell viability was determined by Trypan blue exclusion method. Healthy heifers aged 1.5 to 2 years with recorded vaccination history were used for blood collection by jugular venipuncture (10 mL).…”

Section: Sample Collection and Estimation Of Neutrophil Cell Viabilitymentioning

confidence: 99%

Exploring the concentration‐dependent actions of interferon‐τ on bovine neutrophils to understand the process of implantation

Manjari¹,

Hyder

Kapoor³

et al. 2018

J of Cellular Biochemistry

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Interferon-τ (IFNT) is a major cytokine produced by the ruminant trophoectoderm during the peri-implantation period exerting immunomodulatory actions on various cells including neutrophils. The current in vitro study was undertaken to analyze the concentration-dependent effects of IFNT on neutrophil gene dynamics to understand its possible role in implantation process. The neutrophils were isolated from the blood of heifers and were cultured subjecting them to different IFNT concentrations (1, 5, or 10 ng/mL). The gene-expression patterns of different interferon-stimulated genes, l-selectin, CD31, CD11b, and progesterone-induced blocking factor (PIBF) were analyzed by quantitative real-time PCR. It was observed that at lower concentrations of IFNT, the IFI16, l-selectin, ISG15, and PIBF were upregulated, whereas at higher concentrations the same were down regulated. At all the experimental concentrations, IFI44, OAS1, MX genes were significantly upregulated and CD31, CD11b were significantly downregulated. At lower concentrations of IFNT, the neutrophil activity with respect to chemoattraction is stimulated, whereas at higher concentrations the same is reduced. Hence, it can be concluded that IFNT exerts concentration-dependent actions on neutrophil gene-expression dynamics indicating a fine modulation of its activity depending upon the temporal variation in its destined functions ultimately leading to successful implantation.

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“…As concentrações de corticosterona, 11-desoxicortisol e cortisol apresentaram uma redução marcante de 17,7, 3,5, e 10 vezes, respectivamente O interferon tau (IFNT) liberado pelo trofectoderma altera a expressão gênica no útero e permite o estabelecimento da prenhez (SPENCER et al, 2004). A produção de IFNT se inicia no estágio de blastocisto (HERNANDEZ-LEDEZMA et al, 1992) (PUGLIESI et al, 2014;KIZAKI et al, 2012;GREEN et al, 2010;MANJARI et al, 2016. GIFFORD et al, 2007SOUMYA et al, 2016).…”

Section: Concentração De Corticosteroides Em Fêmeas Bovinas Gestantesunclassified

“…(LENSCHON et al, 2005;NAKAYA et al, 2001 (LENSHOW et al, 2005;NAKAYA et al, 2001), podendo a resposta antiviral ser diferenciada para cada ISG, sugerindo essa como uma das explicações para a marcante diferença encontrada entre os dois genes estudados. (PUGLIESI et al, 2014;KIZAKI et al, 2012;GREEN et al, 2010;MANJARI et al, 2016. GIFFORD et al, 2007SOUMYA et al, 2016 (PUGLIESI et al, 2014;KIZAKI et al, 2012;GREEN et al, 2010;MANJARI et al, 2016.…”

Section: Concentração De Corticosteroides Em Fêmeas Bovinas Gestantesunclassified

Aspectos morfológicos, vasculares e endócrinos de prenhezes produzidas por técnicas de reprodução assistida em bovinos

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Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period

Cited by 25 publications

References 31 publications

Day‐7 embryos generate an anti‐inflammatory immune response in peripheral blood immune cells in superovulated cows

Day‐7 embryos generate an anti‐inflammatory immune response in peripheral blood immune cells in superovulated cows

Exploring the concentration‐dependent actions of interferon‐τ on bovine neutrophils to understand the process of implantation

Aspectos morfológicos, vasculares e endócrinos de prenhezes produzidas por técnicas de reprodução assistida em bovinos

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