Neutrophil Kinetics after Acute Hemorrhage

Gaylor, Michael O.; Chervenick, Paul A.; Boggs, Dane R.

doi:10.3181/00379727-131-34099

Cited by 13 publications

(7 citation statements)

References 4 publications

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“…Trauma and haemorrhagic shock are associated with an acute increase in circulating leucocytes (Thommasen et al 1986;Teggatz et al 1987;Yanagawa et al 2005), which might prevent infection and promote wound healing after tissue damage. Animal studies have evaluated the mechanisms of haemorrhagic leucocytosis (Musser, 1921;Gaylor et al 1969); however, experimental use of invasive methods to induce BL and evaluate haemorrhage-induced leucocytosis is challenging to perform in humans. Animal studies have evaluated the mechanisms of haemorrhagic leucocytosis (Musser, 1921;Gaylor et al 1969); however, experimental use of invasive methods to induce BL and evaluate haemorrhage-induced leucocytosis is challenging to perform in humans.…”

Section: Introductionmentioning

confidence: 99%

White blood cell concentrations during lower body negative pressure and blood loss in humans

Helmond

Johnson

Curry

et al. 2016

Experimental Physiology

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What is the central question of this study? Is lower body negative pressure a useful surrogate to study white blood cell responses to haemorrhage in humans? What is the main finding and its importance? We found that lower body negative pressure appears to be a useful surrogate to study the early white blood cell mobilization response during blood loss. Hypovolaemia has been associated with an immune response that might be secondary to sympathoexcitation. We tested the hypothesis that simulated hypovolaemia using lower body negative pressure (LBNP) and real hypovolaemia induced via experimental blood loss (BL) cause similar increases in the white blood cell concentration ([WBC]). We measured [WBC] and catecholamine concentrations in 12 men who underwent an LBNP and a BL protocol in a randomized order. We compared 45 mmHg of LBNP with 1000 ml of BL; therefore, [WBC] and catecholamine concentrations were plotted against central venous pressure to obtain stimulus-response relationships using the linear regression line slopes for both protocols. Mean regression line slopes were similar for total [WBC] (LBNP 183 ± 4 μl mmHg versus BL 155 ± 109 μl mmHg , P = 0.15), neutrophils (LBNP 110 ± 2 μl mmHg versus BL 96 ± 72 μl mmHg , P = 0.15) and lymphocytes (LBNP 65 ± 21 μl mmHg versus BL 59 ± 38 μl mmHg , P = 0.90). Mean regression line slopes for adrenaline were similar (LBNP 15 ± 5 pg ml mmHg versus BL 16 ± 4 pg ml mmHg , P = 0.84) and were steeper during LBNP for noradrenaline (LBNP 28 ± 6 pg ml mmHg versus BL 9 ± 6 pg ml mmHg , P = 0.01). These data indicate that central hypovolaemia elicits a relative leucocytosis with a predominantly neutrophil-based response. Additionally, our results indicate that LBNP models the stimulus-response relationship between central venous pressure and [WBC] observed during BL.

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Section: Introductionmentioning

confidence: 99%

White blood cell concentrations during lower body negative pressure and blood loss in humans

Helmond

Johnson

Curry

et al. 2016

Experimental Physiology

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“…This pattern bears out the obser vation of R ichard et al [ 17], To be able to understand these changes it must be remembered that bleeding not only produces a loss of red cells but also a loss of granulocytes and platelets. According to G aylor et al [7], after acute hemorrhage, an initial phase of pseudoneutrophilia caused by demargination is followed by an increase in neutrophil production. Therefore, it is possible that the changes of CFU-C observed in this and other studies express a real response of myeloid-commited progenitor cells to a specific, albeit feeble, stimulus to an increased granulopoiesis.…”

Section: Discussionmentioning

confidence: 99%

Effect of Bleeding on <i>in vivo</i> and <i>in vitro</i> Colony-Forming Hemopoietic Cells

et al. 1977

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The effect of bleeding on spleen colony-forming units (CFU-S) and on in vitro colony-forming cells with colony-stimulating factor (CFU-C) and erythropoietin (CFU-E) has been evaluated. The in vivo and in vitro colony-forming cells of the bone marrow show a decrease which for the CFU-E, CFU-C follows a shortlived increase. In the spleen, all progenitor cells assayed have shown a significant and sustained increase

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“…To collect further data on this question the number of neutrophils entering endotoxin-induced peritoneal exudates in mice rendered neutrophilic by bleeding (10) or by infection with the intestinal nematode Nematospiroides dubius (1 1) was compared to normal controls.…”

mentioning

confidence: 99%

“…In mice bled to induce neutrophilia, approximately 0.4 ml of blood was removed from the orbital sinus 2 hr before endotoxin injection (10). Blood leukocyte counts and smears for differential counts (300 cells) were from orbital sinus blood and leukocyte counts were determined electronically (12).…”

mentioning

confidence: 99%

Failure of Neutrophilia to Increase the Number of Neutrophils Entering a Peritoneal Exudate in Mice

Aronoff¹,

Boggs²,

Boggs³

et al. 1975

Experimental Biology and Medicine

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Neutrophil Kinetics after Acute Hemorrhage

Cited by 13 publications

References 4 publications

White blood cell concentrations during lower body negative pressure and blood loss in humans

White blood cell concentrations during lower body negative pressure and blood loss in humans

Effect of Bleeding on <i>in vivo</i> and <i>in vitro</i> Colony-Forming Hemopoietic Cells

Failure of Neutrophilia to Increase the Number of Neutrophils Entering a Peritoneal Exudate in Mice

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