Neutrophil-mediated phospholipid peroxidation assessed by gas chromatography-mass spectroscopy

Abstract

Disease pathophysiology frequently involves manifestations of the systemic inflammatory response syndrome. Oxyradicals represent key inflammatory mediators, and neutrophils are one important source of oxyradicals. This investigation examined neutrophil-mediated peroxidation of dilinoleoyl phosphatidyl-choline (DLPC) liposomes by monitoring the appearance of monohydroxyl linoleic acid with the use of gas chromatography-mass spectroscopy (GC-MS), compared with traditional assessment of thiobarbituric acid-reactive species (TBARS) and phosphatidylcholine-specific conjugated dienes. DLPC was peroxidized in a system using activated neutrophils in balanced salt solution containing chelated iron. 9-Monohydroxyl linoleic acid and 13-monohydroxyl linoleic acid were readily identified in neutrophil-mediated peroxidized DLPC with the use of GC-MS. Neutrophil NADPH oxidoreductase specific activity correlated highly with total ion current or specific ion monitoring of integrated peak areas for peroxidized linoleic acid but correlated poorly with DLPC-derived TBARS or conjugated dienes. These results ascertain that activated neutrophils mediate phosphatidylcholine lipid peroxidation to specific products, which may be precisely monitored with the use of GC-MS. The extent of this peroxidation is highly correlated with the magnitude of the neutrophil respiratory burst.

Protective Effect of Teprenone Against Acute Gastric Mucosal Lesions Induced by Compound 48/80, a Mast Cell Degranulator, in Rats

“…We have observed that teprenone at a concentration of 10 to 100 m g / ml does not inhibit XO activity in gastric mucosal tissue preparations from compound 48 / 80-treated rats (unpublished data). Lipid peroxidation occurs via ROS generated not only by the xanthine-XO system but also by activated NADPH oxidoreductase in neutrophils (39,40). It is also known that MPO mediates lipid peroxidation in the presence of hydrogen peroxide with halide ions (41).…”

Potent Antiulcerogenic Activity of Methanol Extract of Cissus quadrangularis by Antioxidative Mechanism

“…The previous studies indicate that aspirin can promote increase in neutrophil adhesion to venular endothelium in vivo [28]. In addition, it has been reported that neutrophils mediate lipid peroxidation through the production of superoxide anions by the activated NADPH oxidoreductase in the cells [29]. TBARS and tissues associated MPO activity were measured in gastric mucosa as indices of lipid peroxidation and neutrophil infiltration [30].…”

Effect of Oral Vitamin E Administration on Acute Gastric Mucosal Lesion Progression in Rats Treated with Compound 48/80, a Mast Cell Degranulator

“…It is known that activated neutrophils mediate lipid peroxidation via ROS generated by NADPH oxidoreductase. 42) It is also known that MPO mediates lipid peroxidation in the presence of H 2 O 2 with halide ions. 43) Therefore, a part of the aforementioned reduction of gastric mucosal TBARS content in C48/80-treated rats by vitamin E administration may be due to the inhibitory effect of the administered vitamin on neutrophil infiltration into gastric mucosal tissues.…”