Neutrophils are involved in acute, nonspecific resistance to Listeria monocytogenes in mice

Rogers, Howard W.; Unanue, Emil R.

doi:10.1128/iai.61.12.5090-5096.1993

Cited by 236 publications

(100 citation statements)

References 45 publications

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“…Thus infection with live L. monocytogenes is required for the best protection. Furthermore, there are other CD8 + T cell epitopes in L. monocytogenes besides LLO 91-99 [44] and other arms of the immune system such as neutrophils and macrophages that contribute to L. monocytogenes protection [47,48]. However, our DNA vaccine using an SCT elicited a more robust response than live L. monocytogenes infection to the immunodominant LLO 91-99 epitope, demonstrating efficient antigen presentation.…”

Section: Discussionmentioning

confidence: 95%

Single chain MHC I trimer-based DNA vaccines for protection against Listeria monocytogenes infection

Kim

Zuiani

Carrero

et al. 2012

Vaccine

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To circumvent limitations of poor antigen presentation and immunogenicity of DNA vaccines that target induction of CD8+ T cell immunity, we have generated single chain MHC I trimers (MHC I SCTs) composed of a single polypeptide chain with a linear composition of antigenic peptide, β2-microglobulin, and heavy chain of a MHC class I molecule connected by flexible linkers. Because of its pre-assembled nature, the SCT presents enhanced expression and presentation of the antigenic peptide/MHC complexes at the cell surface. Furthermore, DNA vaccination with a plasmid DNA encoding an SCT incorporating an immunodominant viral epitope elicited protective CD8+ T cell responses against lethal virus infection. To extend these findings, here we tested the efficacy of SCT DNA vaccines against bacterial infections. In a mouse infection model of Listeria monocytogenes, the SCT DNA vaccine encoding H-2Kd and the immunodominant peptide LLO 91–99 generated functional primary and memory peptide-specific CD8+ T cells that confer partial protection against L. monocytogenes infection. DNA immunization of Kd/LLO91–99 SCTs generated functional memory CD8+ T cells independently of CD4+ T cells, although the expression of cognate or non-cognate CD4+ helper T cell epitopes further enhanced the protective efficacy of SCTs. Our study further demonstrates that the SCT serves as a potent platform for DNA vaccines against various infectious diseases.

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Section: Discussionmentioning

confidence: 95%

Single chain MHC I trimer-based DNA vaccines for protection against Listeria monocytogenes infection

Kim

Zuiani

Carrero

et al. 2012

Vaccine

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“…Although L. monocytogenes bacteria can induce systemic infection causing spontaneous abortion, septicemia, and meningitis, anti-L. monocytogenes immunity in humans has not been defined. In murine models of systemic L. monocytogenes infection, mice deficient in neutrophils exhibit increased bacterial burden during early L. monocytogenes infection (14)(15)(16). In addition, murine IFN-g, IL-23, IL-17, and CD8 + T cells have been shown to play a role in protection against L. monocytogenes infection (17)(18)(19)(20)(21).…”

mentioning

confidence: 99%

Multieffector-Functional Immune Responses of HMBPP-Specific Vγ2Vδ2 T Cells in Nonhuman Primates Inoculated with Listeria monocytogenes ΔactA prfA *

Ryan-Payseur

Frencher

Shen

et al. 2012

The Journal of Immunology

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Although Listeria monocytogenes can induce systemic infection causing spontaneous abortion, septicemia, and meningitis, studies have not been performed to investigate human anti-L. monocytogenes immune responses, including those of Ag-specific Vγ2Vδ2 T cells, a dominant human γδ T cell subset. L. monocytogenes is the only pathogen known to possess both the mevalonate and non-mevalonate isoprenoid biosynthesis pathways that produce metabolic phosphates or phosphoantigens activating human Vγ2Vδ2 T cells, making it interesting to explore in vivo anti-L. monocytogenes immune responses of Vγ2Vδ2 T cells. In this study, we demonstrated that subclinical systemic L. monocytogenes infection of rhesus macaques via parenteral inoculation or vaccination with an attenuated Listeria strain induced multieffector-functional immune responses of phosphoantigen-specific Vγ2Vδ2 T cells. Subclinical systemic infection and reinfection with attenuated L. monocytogenes uncovered the ability of Vγ2Vδ2 T cells to mount expansion and adaptive or recall-like expansion. Expanded Vγ2Vδ2 T cells could traffic to and accumulate in the pulmonary compartment and intestinal mucosa. Expanded Vγ2Vδ2 T cells could evolve into effector cells producing IFN-γ, TNF-α, IL-4, IL-17, or perforin after L. monocytogenes infection, and some effector Vγ2Vδ2 T cells could coproduce IL-17 and IFN-γ, IL-4 and IFN-γ, or TNF-α and perforin. Surprisingly, in vivo-expanded Vγ2Vδ2 T effector cells in subclinical L. monocytogenes infection could directly lyse L. monocytogenes-infected target cells and inhibit intracellular L. monocytogenes bacteria. Thus, we present the first demonstration, to our knowledge, of multieffector-functional Vγ2Vδ2 T cell responses against L. monocytogenes.

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“…Neutrophils have been found essential for the expression of innate and acquired resistance to listeriosis. [16][17][18][19][20] We showed here that although the activated macrophage generated during the mycobacterial infection was involved in the resistance to listeriosis, it was not able to express its enhanced antimicrobial activity and to control the infection in the absence of the neutrophil.…”

Section: Discussionmentioning

confidence: 99%

“…This antibody has been extensively characterized and has been shown to bind and lyse neutrophils plus eosinophils in vivo and in vitro. [17][18][19][20][21][22] To deplete neutrophils, mice were injected with 200 m g of the antibody 2 hr before the infection with L. monocytogenes. The depletion of neutrophils lasted at least 48 hr.…”

Section: Antibodiesmentioning

confidence: 99%

“…Recently, it has been repeatedly shown that the resistance to murine listeriosis involves not only macrophages but also neutrophils as the effector phagocytes in immunity to this infection. [16][17][18][19][20] The neutrophils are involved both in the early phases of infection as part of innate resistance mechanisms and, in addition, in later phases of the infection when acquired immunity has been generated and in immunized hosts. 20 Thus, it is possible that any type of resistance to murine listeriosis will require the participation of neutrophils.…”

Section: Introductionmentioning

confidence: 99%

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Neutrophils are involved in the non‐specific resistance to listeriosis induced by mycobacterial infections

Leal

Appelberg

Silva³

1996

Immunology

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A major role has been recently ascribed to the neutrophil in the resistance to infection by Listeria monocytogenes (L. monocytogenes). Here we evaluated whether such neutrophils played a role in the non‐specific resistance to listeriosis that develops in hosts infected by mycobacteria. We found that the depletion of neutrophils completely abrogated the resistance conferred by the activated macrophages induced during the mycobacterial infection. The lack of killing by activated Kupffer cells and the visualization of bacteria proliferating inside peritoneal macrophages in neutrophil‐depleted mice allowed us to postulate a role for the cooperation between neutrophils and macrophages in the killing of L. monocytogenes. We also found listerial proliferation in hepatocytes of neutrophil‐depleted, mycobacteria‐infected mice showing that the neutrophils may be involved in the control of listeria infection of parenchymal cells.

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Neutrophils are involved in acute, nonspecific resistance to Listeria monocytogenes in mice

Cited by 236 publications

References 45 publications

Single chain MHC I trimer-based DNA vaccines for protection against Listeria monocytogenes infection

Single chain MHC I trimer-based DNA vaccines for protection against Listeria monocytogenes infection

Multieffector-Functional Immune Responses of HMBPP-Specific Vγ2Vδ2 T Cells in Nonhuman Primates Inoculated with Listeria monocytogenes ΔactA prfA *

Neutrophils are involved in the non‐specific resistance to listeriosis induced by mycobacterial infections

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