Neutrophils as regulators of macrophage-induced inflammation in a setting of allogeneic bone marrow transplantation

Cheng, Hong; Lu, Hongyun; Huang, Xiaohong; Chen, Ming; Jin, Rong; Dai, Xiaoqiu; Gong, Fang‐Yuan; Dong, Han; Wang, Hongmin; Gao, Xiao‐Ming

doi:10.1016/j.stemcr.2022.05.021

Cited by 4 publications

(3 citation statements)

References 50 publications

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“…A lipopolysaccharide (LPS)-induced sepsis model was established via a single intravenous injection of LPS (1 mg/kg, O26:B6; Sigma-Aldrich, St. Louis, MO, USA) ( 22 ). In order to deplete Tregs in vivo , anti-CD25 antibody (100 μg, Clone PC61; BioLegend, San Diego, CA, USA) was injected intravenously 1 day before the CLP model was created ( 23 ).…”

Section: Methodsmentioning

confidence: 99%

Improvement of the sepsis survival rate by adenosine 2a receptor antagonists depends on immune regulatory functions of regulatory T-cells

Zhang

Zhao

et al. 2022

Front. Immunol.

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Adenosine shows a significant immunosuppressive effect in sepsis via binding to the adenosine 2a receptor (A2aR). Both genetic deletion and pharmacological inhibition of the A2aR may improve survival in sepsis. However, available research on this protective mechanism is quite limited. We used an A2aR antagonist (ZM241385) to treat a cecal ligation and puncture model of normal mice or regulatory T-cell (Treg)-depletion mice and found that the protective effect of ZM241385 is dependent on Tregs. Mechanically, A2aR inactivation was associated with decreased frequencies and reduced function of Foxp3+ Tregs, as evidenced by Foxp3 and CTLA-4 expression and classical effector T-cell proliferative assays, suggesting Treg modulation is a potential protective mechanism against sepsis. Simultaneously, the function and quantity of abdominal neutrophils were improved with ZM241385 treatment. To see if a link exists between them, Tregs and neutrophils were co-cultured, and it was found that ZM241385 blocked the inhibitory effect of Tregs on neutrophils. According to our research, Tregs play a key role in how A2aR antagonists improve sepsis prognosis and bacterial clearance.

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Section: Methodsmentioning

confidence: 99%

Improvement of the sepsis survival rate by adenosine 2a receptor antagonists depends on immune regulatory functions of regulatory T-cells

Zhang

Zhao

et al. 2022

Front. Immunol.

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“…Autologous bone marrow transplantation was not recommended in clinical due to the patient’s bone marrow cytopenia or limited osteogenic capacity. − To obtain more and healthier bone marrow cells, allogeneic bone marrow transplantation was also used in the treatment of nonunion and have been proven effective and safe, with similar osteogenic induction ability as autologous bone marrow transplantation . The impediment to allogeneic bone marrow transplantation predominantly attributed to dysregulated immune responses . The process of bone regeneration required the coordinated functioning of several systems, among which the skeletal and immune systems were crucial due to the molecular regulation between them.…”

Section: Resultsmentioning

confidence: 99%

“…47 The impediment to allogeneic bone marrow transplantation predominantly attributed to dysregulated immune responses. 48 The process of bone regeneration required the coordinated functioning of several systems, among which the skeletal and immune systems were crucial due to the molecular regulation between them. Therefore, mitigating the adverse effects of inflammatory cells on allogeneic bone marrow transplantation is beneficial to bone defect repair.…”

Section: Pdots Labeled a Small Number Of Macrophages And Vascular End...mentioning

confidence: 99%

Bone Marrow Stromal Cells Sorted by Semiconducting Polymer Nanodots for Bone Repair

Wang,

Mi,

et al. 2023

ACS Biomater. Sci. Eng.

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The use of bone marrow stromal cells (BMSCs) for bone defect repair has shown great promise due to their differentiation potential. However, isolating the BMSCs from various cell types within the bone marrow remains challenging. To tackle this issue, we utilized semiconducting polymer dots (Pdots) as markers to select the BMSCs within a specific time frame. The therapeutic efficacy of the obtained Pdot-labeled BMSCs was assessed in a bone defect model. Initially, we evaluated the binding capacity of the Pdots with four different types of cells present in the bone marrow including BMSCs, osteoblasts, macrophages, and vascular endothelial cells, in vitro. Notably, BMSCs showed the most rapid uptake of the Pdots, being labeled within only one h of coculture, while other cells took four h to become labeled. Moreover, by colocalizing the Pdots with Prrx1, Sca-1, OSX, F480, and CD105 in the bone marrow cells of monocortical tibial defect (MTD) mice in vivo, we determined the proportions of BMSCs, macrophages, and vascular endothelial cells among all labeled cells from 1 to 8 h after the Pdots injection. It was found that BMSCs have the highest proportion (92%) among all labeled cells extracted after 1 h of Pdots injection. The therapeutic efficacy of the obtained Pdots-labeled BMSCs (1 h) was assessed in a bone defect model. Results showed that the new bone accrual was significantly increased in the treatment of Pdots-labeled BMSCs compared to the bone marrow cell-treated group. Our study revealed that BMSCs screened by the Pdots could improve bone defect repair, suggesting a promising application of the Pdots in bone healing.

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The role of lncRNA NEAT1 in acute graft-versus-host disease: Regulation of macrophage polarization and inflammatory cytokine secretion via JNK/NLRP3 pathway

Huang,

Wang,

et al. 2025

International Immunopharmacology

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Neutrophils as regulators of macrophage-induced inflammation in a setting of allogeneic bone marrow transplantation

Cited by 4 publications

References 50 publications

Improvement of the sepsis survival rate by adenosine 2a receptor antagonists depends on immune regulatory functions of regulatory T-cells

Improvement of the sepsis survival rate by adenosine 2a receptor antagonists depends on immune regulatory functions of regulatory T-cells

Bone Marrow Stromal Cells Sorted by Semiconducting Polymer Nanodots for Bone Repair

The role of lncRNA NEAT1 in acute graft-versus-host disease: Regulation of macrophage polarization and inflammatory cytokine secretion via JNK/NLRP3 pathway

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