Neutrophils in Mycobacterium tuberculosis

Alcantara, Cheldon Ann; Glassman, Ira; Nguyen, Kevin H.; Parthasarathy, Arpitha; Venketaraman, Vishwanath

doi:10.3390/vaccines11030631

Cited by 10 publications

(12 citation statements)

References 70 publications

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“…The immune infiltration analysis demonstrated that macrophages (M1, M2), monocytes, NKTs, neutrophils, Tregs cells, and plasma cells had higher numbers in TB samples in both datasets, which corroborated previous studies [45][46][47][48][49][50]. Human M1 macrophages express unique innate immune response genes after mycobacterial infection to defend against TB, while M2 macrophages have an inhibitory effect on immune responses, inhibiting antigen presentation and T-cell proliferation, exerting anti-inflammatory responses, attenuating cellular immunity against TB infection, and thereby promoting chronic TB infection [51,52].…”

Section: Discussionsupporting

confidence: 90%

Exploring the Role of Extracellular Vesicles in the Pathogenesis of Tuberculosis

Arya,

Shakya,

Chaurasia

et al. 2024

Genes

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Tuberculosis (TB) remains a significant global health concern, necessitating accurate diagnosis and treatment monitoring. Extracellular vesicles (EVs), including exosomes, play crucial roles in disease progression, with their associated genes serving as potential biomarkers and therapeutic targets. Leveraging publicly available RNA-Seq datasets of TB patients and healthy controls (HCs), to identify differentially expressed genes (DEGs) and their associated protein–protein interaction networks and immune cell profiles, the common EV-related DEGs were identified and validated in the GSE42830 and GSE40553 datasets. We have identified nine common EV-related DEGs (SERPINA1, TNFAIP6, MAPK14, STAT1, ITGA2B, VAMP5, CTSL, CEACAM1, and PLAUR) upregulated in TB patients. Immune cell infiltration analysis revealed significant differences between TB patients and HCs, highlighting increased proportions of various immune cells in TB patients. These DEGs are involved in crucial cellular processes and pathways related to exocytosis and immune response regulation. Notably, VAMP5 exhibited excellent diagnostic performance (AUC—0.993, sensitivity—93.8%, specificity—100%), with potential as a novel biomarker for TB. The EV-related genes can serve as novel potential biomarkers that can distinguish between TB and HCs. VAMP5, which functions in exosome biogenesis and showed significant upregulation in TB, can be targeted for therapeutic interventions and treatment outcomes.

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Section: Discussionsupporting

confidence: 90%

Exploring the Role of Extracellular Vesicles in the Pathogenesis of Tuberculosis

Arya,

Shakya,

Chaurasia

et al. 2024

Genes

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“…However, arginase was more highly expressed in groups 1 and 3 than in group 2. Neutrophils are the first defense against bacteria and perform phagocytosis, degranulation and nuclear material release functions in the form of extracellular neutrophil traps (NETs) [ 54 , 55 ]. Arginase-1, released by neutrophil granules, degrades arginine by an immunoregulatory homeostatic mechanism [ 56 ], causing inhibition of cytokine production by NK cells.…”

Section: Discussionmentioning

confidence: 99%

Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses

da Costa,

de Souza Barbosa,

Kipnis

et al. 2023

Vaccines

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The immune response to vaccines is complex and results in various outcomes. BCG vaccination induces innate and specific responses that can lead to protection against tuberculosis, and cross-protection against other infections. NK cells have been associated with BCG-induced protection. Therefore, we hypothesize that differences in NK cell status before BCG vaccination may have a role in the ability of BCG to activate the immune response. Participants of a clinical trial were evaluated after BCG vaccination. The participants were assigned to different groups according to variation in IFN-γ expression by NK cells between days 1 and 15 after BCG vaccination. Individuals that presented a higher increase in IFN-γ expression by NK cells presented reduced CD314 expression at day 1, and after vaccination an increase in inflammatory NK cells and CD4 T-cell expression of IL-17. A negative correlation between expression of CD314 at day 1 and that of IFN-γ by NK cells after BCG vaccination was observed. Participants with lower of IFN-γ expression by NK cells after BCG vaccination presented an increase in the cytotoxic NK subpopulation and CD4 T-cell expression of IL-17 and IFN-γ. In conclusion, the expression of CD314 by NK cells before BCG vaccination influences their IFN-γ responses, generation of NK subpopulations, and the specific T immune response at 15 days after vaccination.

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“…In the oxygen‐independent killing pathway, neutrophil granules also play a crucial role in inhibiting or killing MTB 27 . Cationic proteins, defensins, and permeability proteins are the major bactericidal substances, working by direct contact with MTB to eliminate it 28 . Furthermore, activated neutrophils secrete various chemotactic factors, such as IL‐8, CXCL8, CXCL1, CXCL10, and CXCL11, which amplify their own recruitment and directed chemotaxis, synergistically working with other immune cells to enhance phagocytosis and bactericidal functions, thus enhancing the host's killing effect on MTB 29,30 …”

Section: Immunologic Mechanisms Of Mtb–host Interactionsmentioning

confidence: 99%

Mycobacterium tuberculosis: immune response, biomarkers, and therapeutic intervention

Zhuang,

Yang,

et al. 2024

MedComm

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Although tuberculosis (TB) is an infectious disease, the progression of the disease following Mycobacterium tuberculosis (MTB) infection is closely associated with the host's immune response. In this review, a comprehensive analysis of TB prevention, diagnosis, and treatment was conducted from an immunological perspective. First, we delved into the host's immune response mechanisms against MTB infection as well as the immune evasion mechanisms of the bacteria. Addressing the challenges currently faced in TB diagnosis and treatment, we also emphasized the importance of protein, genetic, and immunological biomarkers, aiming to provide new insights for early and personalized diagnosis and treatment of TB. Building upon this foundation, we further discussed intervention strategies involving chemical and immunological treatments for the increasingly critical issue of drug‐resistant TB and other forms of TB. Finally, we summarized TB prevention, diagnosis, and treatment challenges and put forward future perspectives. Overall, these findings provide valuable insights into the immunological aspects of TB and offer new directions toward achieving the WHO's goal of eradicating TB by 2035.

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Neutrophils in Mycobacterium tuberculosis

Cited by 10 publications

References 70 publications

Exploring the Role of Extracellular Vesicles in the Pathogenesis of Tuberculosis

Exploring the Role of Extracellular Vesicles in the Pathogenesis of Tuberculosis

Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses

Mycobacterium tuberculosis: immune response, biomarkers, and therapeutic intervention

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