Neutrophils: Interplay between host defense, cellular metabolism and intracellular infection

Rane, Deepti; Patil, Tejaswini; More, Vasundhara; Patra, Sushree Sangita; Bodhale, Neeelam; Dandapat, Jagneswar; Sarkar, Arup

doi:10.1016/j.cyto.2018.07.009

Cited by 7 publications

(6 citation statements)

References 86 publications

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“…At the infected sites, they destroy invading pathogens through phagocytosis, respiratory burst activity, the release of granule contents, and extracellular traps [47]. These microenvironmental and functional changes are accompanied by metabolic changes that have a direct role in regulating neutrophil function [48,49].…”

Section: Cellular Metabolism and Function Of Neutrophilsmentioning

confidence: 99%

Glycogen storage disease type Ib: role of glucose‐6‐phosphate transporter in cell metabolism and function

et al. 2019

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Cellular metabolism generally refers to biochemical processes that produce or consume energy within the cell. Recent studies have established that aberrant metabolic states caused by internal or external stresses and genetic mutations are intertwined with several human pathologies. Gaining insight into these metabolic alterations is, therefore, essential for understanding the pathophysiology of various diseases. Glycogen storage disease type Ib (GSD-Ib) is an autosomal recessive disorder characterized by hypoglycemia, excessive glycogen accumulation in the liver and kidney, neutropenia, neutrophil dysfunction, and inflammatory bowel disease. GSD-Ib is caused by a deficiency of glucose-6-phosphate transporter (G6PT). Recently, it was reported that deficiency of G6PT also leads to the aberrant proliferation and differentiation of mesenchymal stem cells and impaired regulatory T-cell function. This review describes the broad impact of altered cellular metabolism resulting from a lack of G6PT activity on cellular function and considers the prospects of developing novel approaches for GSD-Ib treatment.

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Section: Cellular Metabolism and Function Of Neutrophilsmentioning

confidence: 99%

Glycogen storage disease type Ib: role of glucose‐6‐phosphate transporter in cell metabolism and function

et al. 2019

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“…Cytochemical test for cationic lysosomal proteins determines the presence of oxygen-independent mechanisms of bactericidal neutrophils. The group of these proteins includes α-defensins, which are synthesized and packaged in granules during neutrophil differentiation [21,22]. We found that in acute destructive inflammation of AO slightly increased percentage of neutrophils containing cationic lysosomal proteins, while in other pathologies the indicator did not differ from the control value.…”

Section: Discussion Of Research Resultsmentioning

confidence: 86%

Functional activity of blood neutrophiles in acute inflammatory diseases of the abdominal organs and abdominal tuberculosis

Акімова¹,

Lapovets²,

Lapovets³

et al. 2020

ScienceRise: Medical Science

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The diagnosis of various pathomorphological forms of acute appendicitis (AA), acute mesadenitis (AM), and abdominal tuberculosis (AT) remains an urgent problem in medicine. Neutrophilic granulocytes are the first link in the nonspecific immune response to inflammation, which is mediated by phagocytosis and intracellular bactericidal systems. Therefore, the research aimed to establish the features of the functional state of neutrophilic granulocytes in the blood of patients with AA, AM, and AT. Materials and methods. 30 practically healthy, 27 patients with AM, 40 patients with acute phlegmonous appendicitis were examined; 20 patients with acute gangrenous appendicitis, 30 patients with AT. The phagocytic activity of neutrophils in the test with latex granules, redox activity (spontaneous HCT test), cationic lysosomal proteins in the cytochemical test with bromine phenol blue was determined in the blood. Results. Phagocytic activity of neutrophils in patients with destructive forms of AA and AT was lower, and in the group of patients with AMdid not differ from control values. The phagocytic index of neutrophils in patients with destructive forms of AA was 1.5 times lower (p<0.05), and the phagocytic number in patients with gangrenous AA was 1.7 times lower than in healthy individuals (p<0.05). In patients with AM, the percentage of HCTpositive neutrophils was 1.3 times higher, in patients with destructive forms of AA and AT -2 times higher than in healthy groups (p<0.05). The number of neutrophils containing cationic lysosomal proteins was also higher in destructive forms of AA, and in AM and AT -did not differ from the value in the control group. Conclusions. It has been established that peripheral blood neutrophils in conditions of acute destructive inflammation and abdominal tuberculosis have reduced absorption capacity with simultaneous significant activation of redox processes. Cytochemical tests to detect the phagocytic and metabolic activity of blood neutrophils are available and highly informative for the diagnosis and prediction of inflammation, as well as for the differential diagnosis of bacterial and viral diseases

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“…Previous studies have indicated that neutrophils primarily rely on glucose as their primary carbon source for metabolic processes [40]. However, emerging evidence suggests that neutrophils also utilize a variety of other nutrients, including amino acids, carbohydrates, proteins, and lipids, to generate energy [41,42]. Neutrophils frequently encounter immunological environments with limited nutrient availability, necessitating their ability to adapt and employ diverse metabolic pathways to meet the demands of the immune response.…”

Section: Discussionmentioning

confidence: 99%

Fatty acid metabolism in neutrophils promotes lung damage and bacterial replication during tuberculosis

Sankar,

Ramos,

Corro

et al. 2023

Preprint

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Mycobacterium tuberculosis(Mtb) infection triggers a significant influx of neutrophils to the lungs, which is linked to tuberculosis (TB) severity. The mechanism by which Mtb infection induces neutrophillic inflammation remains unclear. Using a clinically relevant and hypervirulent Mtb strain from the W-Beijing family, HN878, we found that genes related to both glycolysis and fatty acid metabolism are upregulated in the lung neutrophils of susceptible mice. Similar effects in gene expression were observed in rabbits, and humans with pulmonary TB compared to healthy controls. Inhibiting glycolysis with 2-deoxy D-glucose (2-DG) exacerbated disease pathology, while fatty acid oxidation (FAO) inhibitor Etomoxir (ETO) improved outcomes by reducing weight loss, immunopathology, and bacterial replication within neutrophils in genetically susceptible mice. Notably, ETO reduced neutrophil production in the bone marrow and their recruitment to the lungs. ETO specifically restrained the recruitment of Ly6Glow/dimimmature neutrophil population, which is elevated during disease progression and harbors the bulk of bacilli. In a transwell setup, we demonstrated that ETO dose-dependently inhibited neutrophil chemotaxis towards infected macrophages. In summary, our research highlights the crucial role of fatty acid metabolism in regulating neutrophilic inflammation during TB and provides a rationale for targeting immunometabolism of neutrophils for potential TB treatment.

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Neutrophils: Interplay between host defense, cellular metabolism and intracellular infection

Cited by 7 publications

References 86 publications

Glycogen storage disease type Ib: role of glucose‐6‐phosphate transporter in cell metabolism and function

Glycogen storage disease type Ib: role of glucose‐6‐phosphate transporter in cell metabolism and function

Functional activity of blood neutrophiles in acute inflammatory diseases of the abdominal organs and abdominal tuberculosis

Fatty acid metabolism in neutrophils promotes lung damage and bacterial replication during tuberculosis

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