Neutrophils secrete MIP‐1β after adhesion to laminin contained in basement membrane of blood vessels

Chiba, Kazuhiro; Zhao, Wenran; Chen, Jiang; Wang, Jingxin; Cui, Hong; Kawakami, Hiroshi; Miseki, Tetsuya; Hashino, Satoshi; Tanaka, Junji; Asaka, Masahiro; Kobayashi, Masanobu

doi:10.1111/j.1365-2141.2004.05242.x

Cited by 21 publications

(13 citation statements)

References 16 publications

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“…Freshly-isolated neonatal and adult neutrophils stimulated with LPS had marked secretion of MIP-1 ␤ /CCL4, a chemokine involved in the recruitment of monocytes and neutrophils to inflammatory sites [43,46] , and is an observation consistent with existing literature [47,48] . We also now report that surviving neonatal neutrophils could be induced to secrete MIP-1 ␤ .…”

Section: Discussionsupporting

confidence: 73%

Neonatal Neutrophils with Prolonged Survival Secrete Mediators Associated with Chronic Inflammation

Nguyen

Schnulle²,

Chegini³

et al. 2010

Neonatology

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Background: The resolution of inflammation involves the efficient removal of apoptotic neutrophils (PMN). However, a subpopulation of PMN that are resistant to apoptosis may contribute to PMN persistence in tissues, an early hallmark of chronic inflammation. We previously made observations that neonatal PMN with prolonged survival had augmented expression of CD18/CD11b, an adhesion molecule critical to inflammation. Objectives: The objectives of this study were to test the hypothesis that surviving neonatal PMN retain the capacity to secrete key mediators associated with chronic inflammation. Methods: We profiled cytokine and chemokine secretion patterns of lipopolysaccharide (LPS)-stimulated neonatal and adult PMN using multicytokine array and ELISA. Results: We observed that surviving 24-hour neonatal PMN stimulated with LPS had enhanced secretion of interleukin (IL)-8, a chemokine involved in PMN activation and recruitment. In addition, 24-hour neonatal PMN secreted levels of monocyte inhibitory protein (MIP)-1β that were higher than those secreted by 0-hour PMN, but amounts of IL-1 receptor antagonist (IL-1Ra) were lower. Conclusions: The results of the present study extend previous observations of augmented function in surviving neonatal neutrophils, and further suggest their potential contribution to the pathogenesis of inflammatory disorders in neonates.

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Section: Discussionsupporting

confidence: 73%

Neonatal Neutrophils with Prolonged Survival Secrete Mediators Associated with Chronic Inflammation

Nguyen

Schnulle²,

Chegini³

et al. 2010

Neonatology

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“…Other neutrophil-derived chemokines such as MIP-1 , however, act on macrophages, T-cells and dendritic cells [25], and are considered to play a role in the regulation of immune responses [5]. Assessment of the MIP-1 content in PMN supernatants revealed that exposure to 10 ng/ml of MALP-2 induced the release of high amounts of MIP-1 in cultures of neutrophil granulocytes (Fig.…”

Section: Resultsmentioning

confidence: 98%

Direct stimulatory effects of the TLR2/6 ligand bacterial lipopeptide MALP-2 on neutrophil granulocytes

Wilde

Lotz

Engelmann

et al. 2006

Med Microbiol Immunol

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Bacterial lipopeptides represent a group of bacterial compounds able to trigger the functions of cells of the innate immune response. Whereas diacylated lipopeptides are recognized by TLR2/6 dimers, triacylated lipopeptides were shown to act via TLR2/1 dimers. Although several previous studies dealt with the effect of the TLR2/1 ligand Pam(3)CysSK(4) on neutrophil granulocytes (PMN), it is still not clear whether TLR2/6 ligand lipopeptides can directly influence PMN functions. In the present study we used highly purified human neutrophils to investigate the direct effects of the diacylated mycoplasmal macrophage activating lipopeptide-2 (MALP-2) on the function of neutrophil granulocytes. After exposure to 10 ng/ml MALP-2 neutrophils acquired activated cell shape, secreted IL-8 and MIP-1beta and their phagocytic capacity was enhanced. Analysis of cell surface activation markers confirmed the activating effect of MALP-2, the expression of CD62L was downregulated whereas CD11b was upregulated on PMN after exposure to MALP-2. The constitutive apoptosis of PMN was inhibited after exposure to MALP-2. However, MALP-2 exerted only a short-term effect on the apoptosis of resting neutrophils, a longer lasting effect was observed after transendothelial migration. MALP-2 did not directly induce the production of reactive oxygen intermediates but primed PMN for a fMLP-induced oxidative burst. The migration of neutrophils was enhanced after treatment with MALP-2. This was due, however, to a chemokinetic rather than to a chemotactic effect. Pam(3)CysSK(4) also activated PMN, but in comparison to MALP-2, at higher concentrations. These findings suggest that diacylated lipopeptides are important microbial structures recognized by and acting on neutrophil granulocytes.

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“…GRO can also stimulate the production of O 2 − by neutrophils [22], which would also explain the increased production of O 2 − by neutrophils after the intense exercise. MCP-1 can be secreted by activated neutrophils [4,35,36,40]. No previous studies have been made on the effect of acute exercise on MCP-1 levels in healthy sedentary women.…”

Section: Discussionmentioning

confidence: 97%

A single session of intense exercise improves the inflammatory response in healthy sedentary women

et al. 2010

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Physical activity has a great capacity for modulating the immune system, including the inflammatory response. However, the effects of exercise on the inflammatory response have rarely been studied in women, even though women are more susceptible than men to chronic inflammatory diseases. The aim of this study was to ascertain the effect of single sessions of exercise on the inflammatory response of sedentary women, evaluating neutrophil function and circulating concentrations of inflammatory cytokines. Exercise consisted of one session of cycling (1 h at ~70% of VO₂ max) on a cycle ergometer. Blood samples were taken in the basal state and immediately after the exercise session. Neutrophil function was studied on isolated cells by evaluating their phagocytic capacity against latex beads and their oxygen-dependent microbicidal capacity as reflected in the superoxide anion (O₂⁻) production. Circulating inflammatory cytokines were determined using a novel antibody-based protein micro-array method. The circulating concentration of IL-8 (a stimulatory cytokine for neutrophils) was also determined by ELISA. Exercise increased the phagocytic and the oxygen-dependent microbicidal capacities of neutrophils in the sedentary women. No variations were found in IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, IL-10, IL-13, IFN-γ, TNF-α and -β, TGF-β, MCP-2 and -3, MIG, G-CSF, and GM-CSF. However, while the circulating concentrations of GRO and MCP-1 increased after exercise, there was a decrease in that of RANTES, a pro-inflammatory cytokine. Exercise improves neutrophil function, possibly mediated, at least partially, by GRO (a potent neutrophil activator) but not by IL-8. This stimulation of neutrophil function does not seem to be accompanied by any harmful systemic inflammatory response since no changes in the main pro-inflammatory cytokines were observed, and there was a decrease in RANTES.

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Neutrophils secrete MIP‐1β after adhesion to laminin contained in basement membrane of blood vessels

Cited by 21 publications

References 16 publications

Neonatal Neutrophils with Prolonged Survival Secrete Mediators Associated with Chronic Inflammation

Neonatal Neutrophils with Prolonged Survival Secrete Mediators Associated with Chronic Inflammation

Direct stimulatory effects of the TLR2/6 ligand bacterial lipopeptide MALP-2 on neutrophil granulocytes

A single session of intense exercise improves the inflammatory response in healthy sedentary women

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