Nevirapine mitigates monosodium glutamate induced neurotoxicity and oxidative stress changes in prepubertal mice

Abstract

Aim:We investigated the effects of nevirapine on changes in body weight, lipid peroxidation/antioxidant status, apoptotic markers and neuromorphology following monosodium glutamate-induced neurotoxicity in prepubertal mice. Material and Methods: Eighty male mice which were randomly divided into eight groups of ten mice each (n=10) were used. Mice in each group were administered oral vehicle, monosodium glutamate (MSG) at 2g/kg, or one of three doses of nevirapine (at 7.5, 15 and 30 mg/kg) alone or co-administered with MSG. Vehicle or nevirapine were administered daily for 28 days, while MSG was administered on days 1-7. On day 28, animals were euthanized and blood was collected for estimation of plasma malondialdehyde (MDA) and antioxidant levels; while sections of the cerebrum and hippocampus were either fixed and processed for general histology, or homogenized for the estimation of brain biochemical parameters. Results: Results showed that administration of nevirapine alone (or when co-administered with MSG) was associated with a reduction in weight gain, increase in plasma/brain MDA levels, morphologic/morphometric evidence of dose-related hypercellularity; and varying degrees of neuroprotection, with co-administration. Nitric oxide levels and caspase-3 activity increased only with MSG, while superoxide dismutase activity decreased. Conclusions: In conclusion, subchronic administration of nevirapine was associated with dose-related alterations of the measured parameters; indicating possible neuroprotection and mitigation of MSG neurotoxicity at some of the doses studied.

An Assessment of the Effects of Azodicarbonamide-containing Diet on Neurobehaviour, Brain Antioxidant Status and Membrane Lipid Peroxidation Status in Rats

“…Lipid peroxidation levels measured as malondialdehyde content in whole-brain homogenate were determined as previously described [ 17 ]. Supernatant from homogenised brain tissue was transferred into a tube to which standard solution was added, followed by the addition of 2 mL of thiobarbituric acid.…”

Corylus avellana L. modulates neurobehaviour and brain chemistry following high-fat diet

“…Superoxide dismutase activity was determined using commercially-available assay kit. Colour changes were measured at an absorbance of 560 nm as described previously (22). Nitric oxide assay was carried out using the Nitric oxide kit as previously described (17).…”