New Activities for Isolated Compounds from<i>Convolvulus austro-aegyptiacus</i>as Anti-ulcerogenic, Anti-<i>Helicobacter pylori</i>and Their Mimic Synthesis Using Bio-guided Fractionation

Awaad, Amani S.; Al-Rifai, Asma’a; El‐Meligy, Reham M.; Alafeefy, Ahmed M.; Zain, Mohamed E.

doi:10.1002/ptr.5379

Cited by 23 publications

(10 citation statements)

References 23 publications

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“…Many Convolvulus species are valuable ornamentals, medicinal and food crops [ 16 ]. They possess cytotoxic [ 17 ], antioxidant, anti-inflammatory, antiasthma, antijaundice [ 18 ], anticancer, and antiulcer activities [ 19 ]. In addition, they can be used in treatment of coughs and asthma [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial, Antioxidant Activity of Ethanolic Plant Extracts of Some Convolvulus Species and Their DART‐ToF‐MS Profiling

Al-Rifai

Aqel

Al‐Warhi

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Convolvulus austroaegyptiacus Abdallah & Sa'ad (CA) and Convolvulus pilosellifolius Desr. (CP) are commonly used in the Saudi Arabia folk medicine. They are potent in treating the ulcers and skin diseases. The lack of information about their biological activities led us to investigate the possible biological activities by determination of antibacterial and antioxidant activities of total ethanolic extracts and various fractions. Total flavonoid contents of the plants were determined by colorimetric method while total phenols were determined by using Folin-Ciocalteu method. In vitro antibacterial activity was studied against E. coli, P. aeruginosa, and B. subtilis, and the total antioxidant capacity was evaluated by radical scavenging method. IC50 were found to be 21.81, 17.62, and 3.31 μg/mL for CA, CP, and vitamin C, respectively, while the lowest MIC value of 0.25 mg/mL was recorded with CP extract against B. subtilis. Around 21 compounds are tentatively elucidated from both plants using rapid, simple, and high-resolution analytical technique for chemical profiling of natural compounds by direct analysis in real-time of flight-mass spectrometry, of which 17 were not isolated or reported previously.

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Section: Introductionmentioning

confidence: 99%

Antibacterial, Antioxidant Activity of Ethanolic Plant Extracts of Some Convolvulus Species and Their DART‐ToF‐MS Profiling

Al-Rifai

Aqel

Al‐Warhi

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…One possible approach to treating H. pylori infections is to control the action of urease by using urease inhibitors (Awaad et al. 2015 ). Based on our results, isoimperatorin is a potential agent for antiulcer drug development.…”

Section: Resultsmentioning

confidence: 99%

“…In the third group, isoimperatorin was orally administered at the concentration of 40 mg/kg bw, and 30 min later, absolute ethanol (1 mL/200 g bw) was administered orally (Awaad et al. 2015 ). Animals were sacrificed at 1 h after ethanol administration.…”

Section: Methodsmentioning

confidence: 99%

Isolation, characterization, and in silico , in vitro and in vivo antiulcer studies of isoimperatorin crystallized from Ostericum koreanum

Raza

Abbas

Hassan

et al. 2016

Pharmaceutical Biology

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Context:Ostericum koreanum (Maxim.) Kitagawa (Apiaceae) roots are traditionally used as an analgesic and antiulcer agent. However, the antiulcer potential of isoimperatorin isolated from O. koreanum has not yet been explored.Aim: To evaluate the antiulcer activity of isoimperatorin isolated from the roots of O. koreanum.Materials and methods: Isoimperatorin was isolated as cubic crystals by repeated column chromatography of the ethyl acetate fraction and structure was verified with 1H NMR, 13C NMR and high-resolution mass spectrometry (HRMS-FAB). The crystals obtained were analyzed with the single crystal X-ray method. The MTT assay was used to determine its cytotoxicity against chondrocytes at different concentrations (0.0–737.74 μM, 24 h). The in vivo antiulcer activity of isoimperatorin (40 mg/kg) was determined against ethanol-, indomethacin- and pyloric ligation-induced ulcers in Sprague-Dawley rats. Furthermore, the effect of isoimperatorin (0.0–737.74 μM, 24 h) on the expression of type II collagen in chondrocytes was determined using western blot method. The in vitro urease inhibitory activity of isoimperatorin (0–80 μM) and molecular docking was also performed against urease.Results and discussion: Isoimperatorin demonstrated significant inhibitory activity (IC50 36.43 μM) against urease as compared to the standard drug thiourea (IC50 33.57 μM) without cytotoxic effects. It provided 70.9%, 67.65% and 54.25% protection in ulcer models induced by ethanol, indomethacin and pyloric ligation, respectively. Isoimperatorin showed the highest expression level of type II collagen at 368.87 μM. The docking results confirmed strong binding affinity with the target protein.Conclusion: Isoimperatorin may be used to develop antiulcer drugs with decreased side effects.

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“…Compounds 3 and 4 were isolated and identified as kaempferol and quercetin using different instrumental analysis including 1 H‐NMR, 13 C‐NMR spectra and comparison with published literatures (Awaad et al ,; Zain et al , ; Bayoumi et al , ).…”

Section: Resultsmentioning

confidence: 99%

“…It comprises 250 species present as trees, shrubs or herbs. Most of Convolvulus plants contain alkaloids (Nacef et al , ), anthocyanin (Cabrita, ), flavanoids and coumarins (Awaad et al , ; Sethiya et al , ). In addition, they possess many pharmacological activities including cytotoxic (Arora & Malhotra, ), antioxidant, antiinflammatory and antiulcer activities (Sethiya et al , ), used in treatment of coughs and asthma (Donia et al , ).…”

Section: Introductionmentioning

confidence: 99%

Novel Compounds with new Anti‐Ulcergenic Activity from Convolvulus pilosellifolius Using Bio‐Guided Fractionation.

Awaad

Al-Refaie

El‐Meligy

et al. 2016

Phytotherapy Research

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Oral administration of the total alcohol extract of Convolvulus pilosellifolius Desr. (250 and 500 md/kg) showed potent anti-ulcerogenic activity in absolute ethanol-induced ulcer model in rats; it showed percent protection of control ulcer by 69.2 and 84.6%, respectively, while standard ranitidine (100 mg/kg) exhibited 46.2%. Bio-guided work leads to isolation of two novel compounds (1 and 2), which were identified through H, C NMR, HMPC, HMQC and DEPT as: methyl 2-(hydroxymethyl) octanoate, named as amanitate, and 16-amino-9,13-dimethyl-17-(prop-1-en-2-yl)-hexadecahydro-1H-cyclopenta[a] phenanthren-3-ol, named as asmatol. Both compounds (50 mg/kg) possessed anti-ulcerogenic activity with 95.4% and 55.84% protection, respectively. Two known compounds (3 and 4) were also isolated and identified through comparison with authentic samples and confirmed through different NMR techniques as kampeferol and quercetin. These compounds also showed anti-ulcerogenic activity with 78.38% and 5.38% protection, respectively. The cytoprotective mechanism explains the potent anti-ulcerogenic activity of the total alcohol extract and the isolated compounds. The extract was highly safe as the LD was more than 5000 mg/kg. These results were well supported by the sub-chronic toxicity study, as the extract (500 mg/kg) administrated orally to rats for 35 consecutive days showed no alteration in the liver and kidney functions. Copyright © 2016 John Wiley & Sons, Ltd.

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New Activities for Isolated Compounds fromConvolvulus austro-aegyptiacusas Anti-ulcerogenic, Anti-Helicobacter pyloriand Their Mimic Synthesis Using Bio-guided Fractionation

Cited by 23 publications

References 23 publications

Antibacterial, Antioxidant Activity of Ethanolic Plant Extracts of Some Convolvulus Species and Their DART‐ToF‐MS Profiling

Antibacterial, Antioxidant Activity of Ethanolic Plant Extracts of Some Convolvulus Species and Their DART‐ToF‐MS Profiling

Isolation, characterization, and in silico , in vitro and in vivo antiulcer studies of isoimperatorin crystallized from Ostericum koreanum

Novel Compounds with new Anti‐Ulcergenic Activity from Convolvulus pilosellifolius Using Bio‐Guided Fractionation.

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