New amino acid based biodegradable poly(ester amide)s <i>via</i> bis-azlactone chemistry

Kobauri, Sophio; Otinashvili, Giuli; Kantaria, Tengiz; Tugushi, David; Kharadze, Darejan; Kutsiava, N.; Puiggalı́, Jordi; Katsarava, Ramaz

doi:10.1080/10601325.2018.1513776

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…It has to be noted here that molecular weights of heterochain polymers measured in HFIP/ CF 3 COONa system are 2−3 times lower than molecular weights determined in dimethylformamide (DMF). 49 Our attempt to determine the molecular weights of the CPs using the GPC in DMF failed because the polymers were irreversibly bound to Styragel columns.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Library of Cationic Polymers Composed of Polyamines and Arginine as Gene Transfection Agents

et al. 2019

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Combinatorially synthesized materials, especially cationic polymers (CPs), with gene transfection function hold great promise in nanotechnology. However, the main limitations of the existing CPs [such as polyethylenimine (PEI), poly-L-arginine, or polyamidoaminebased dendrimers] as gene transfection agents are high cytotoxicity in the physiological environment. We have developed novel CPs composed of polyaminesendogeneous tetraamine spermine (Spm) and synthetically made triamine N-(2-aminoethyl)-1,3-propanediamine (Apd) for incorporating sec-amino groups and imparting PEI-like structure to the CP backbones. Naturally occurring building blocks such as amino acid arginine (R) was also used for incorporating guanidine-groups into the CPs. The cytotoxicity of resulting CPspolyureas (PUs) and polyamides such as polysuccinamides and R-attached polymalamides was evaluated using murine and human fibroblasts and carcinoma cell lines. The cell compatibility screening revealed that the CPs made of Apd are less cytotoxic compared to Spm-based analogues. From the novel polymer library, total of six polymers were further studied for oligonucleotide (pDNA) complexation and transfection abilities. Highly water-soluble CPs formed nano-sized polyplexes with pDNA at rather low CP/pDNA weight ratios and showed less cytotoxicity and higher transfection ability compared to widely used PEI as well as commercially available transfection agents. Furthermore, new CPs showed selective transfection activity toward certain cell lines (4T1, HeLa, NIH3T3, and CCD 27SK), which is important for their potential applications in gene therapy.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Library of Cationic Polymers Composed of Polyamines and Arginine as Gene Transfection Agents

et al. 2019

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Drug release behavior of poly(amino acid)s drug-loaded nanoparticles with pH-responsive behavior

Shen,

Wang,

et al. 2023

Journal of Drug Delivery Science and Technology

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Artificial Polymers made of α-amino Acids - Poly(Amino Acid)s, Pseudo-Poly(Amino Acid)s, Poly(Depsipeptide)s, and Pseudo-Proteins

Zavradashvili

Puiggalı́

Katsarava

2020

CPD

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Degradable polymers (DPs) - “green materials” of the future, have an innumerable use in biomedicine, particularly in the fields of tissue engineering and drug delivery. Among these kind of materials naturally occurring polymers - proteins which constituted one of the most important “bricks of life” - α-amino acids (AAs) are highly suitable. A wide biomedical applicability of proteins is due to special properties such as a high affinity with tissues and releasing AAs upon biodegradation that means a nutritive potential for cells. Along with these positive characteristics proteins as biomedical materials they have some shortcomings, such as batch-to-batch variation, risk of disease transmission, and immune rejection. The last limitation is connected with the molecular architecture of proteins. Furthermore, the content of only peptide bonds in protein molecules significantly restricts their material properties. Artificial polymers with the composition of AAs are by far more promising as degradable biomaterials since they are free from the limitations of proteins retaining at the same time their positive features - a high tissue compatibility and nutritive potential. The present review deals with a brief description of different families of AA-based artificial polymers, such as poly(amino acid)s, pseudo-poly(amino acid)s, polydepsipeptides, and pseudo-proteins - relatively new and broad family of artificial AA-based DPs. Most of these polymers have a different macromolecular architecture than proteins and contain various types of chemical links along with NH-CO bonds that substantially expands properties of materials destined for sophisticated biomedical applications.

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New amino acid based biodegradable poly(ester amide)s via bis-azlactone chemistry

Cited by 3 publications

References 36 publications

Library of Cationic Polymers Composed of Polyamines and Arginine as Gene Transfection Agents

Library of Cationic Polymers Composed of Polyamines and Arginine as Gene Transfection Agents

Drug release behavior of poly(amino acid)s drug-loaded nanoparticles with pH-responsive behavior

Artificial Polymers made of α-amino Acids - Poly(Amino Acid)s, Pseudo-Poly(Amino Acid)s, Poly(Depsipeptide)s, and Pseudo-Proteins

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