New and effective EGFR-targeted fluorescence imaging technology for intraoperative rapid determination of lung cancer in freshly isolated tissue

Application of Epithelial Growth Factor Receptor-Targeted Magnetic Resonance Imaging and Near-Infrared II Dual-Modal Probe in Lung Cancer Diagnosis and Surgical Resection

“…4,5 Probes targeting EGFR have been validated on ex vivo samples and can accurately identify tumors. 6 MRI has tremendous potential in preoperatively diagnosing lung cancer. Unlike X-ray, CT, or PET-CT, MRI stands out owing to its high soft tissue resolution and lack of radiation, rendering it conducive for multiple repeated examinations during treatment.…”

“…With its strong penetration and excellent imaging capabilities, NIR-II fluorescence has demonstrated the specific identification ability for intrapulmonary tumors in previous studies. 6,15,16 The combination of MRI and NIR-II imaging is used to meet the current clinical diagnostic and therapeutic needs. For patients with advanced-stage lung cancer, neoadjuvant therapy has gradually become the standard treatment.…”

“…To address this issue, a research study showed that targeted probes can detect an additional 33% of cancer lesions . Epidermal growth factor receptor (EGFR) is a confirmed cellular target, demonstrating increased expression in a broad spectrum of human neoplasms, including intracranial neoplasm, breast carcinoma, and, notably, pulmonary carcinoma, wherein it is intricately linked to tumorigenesis, metastasis, and neovascularization processes. , Probes targeting EGFR have been validated on ex vivo samples and can accurately identify tumors …”

NIR-II fluorescence imaging-guided colorectal cancer surgery targeting CEACAM5 by a nanobody

“…Near-infrared (NIR) fluorescence imaging is a real-time imaging technology that combines near-infrared fluorescent targeted probes with a fluorescence imaging system to distinguish tumour from normal tissue by identifying specific and highly expressed molecular targets on tumour tissue. 11 , 12 , 13 Several clinical studies of NIR-targeted probes have been reported for malignancies (colorectal, pancreatic, and ovarian), 14 , 15 , 16 metastases (liver and peritoneum), 17 , 18 and vital anatomical structures (nerves, etc.). 19 Conventional fluorescence-guided surgery focuses on the first near-infrared window (NIR-I, 700–900 nm), which has limited tissue penetration depth of only 1–6 mm.…”

Hypsochromic Shift Donor–Acceptor NIR-II Dye for High-Efficiency Tumor Imaging

“…Theoretically, coupling strong electron-donating groups with strong acceptor groups can ensure efficient NIR-II emission . BODIPY, rhodamine, and cyanine-based D-A NIR-II dyes with good photophysical properties have been developed, showing great potential as NIR-II imaging agents. − However, they always suffer from the problem of poor hydrophilicity, photostability, and fast elimination. , Based on the classical D-A-D dye structure, D-A polymers were also developed with a more significant Stokes shift and better extinction coefficient, but the enormous molecular weight, hard to control degree of polymerization, and the potential long-term retention toxicity limit their clinical transportation. − D-A NIR-II small molecular dyes that reduce one donor group from the D-A-D skeleton have a more simplified structure with a more significant Stokes shift, better photostability, and easier metabolism ability. However, few of these D-A dyes have been reported so far.…”