New antimicrobial activity for the catecholamine release-inhibitory peptide from chromogranin A

Briolat, Jenny; Wu, Shujuan; Mahata, Sushil K.; Gonthier, B.; Bagnard, Dominique; Chasserot‐Golaz, Sylvette; Helle, Karen B.; Aunis, Dominique; Metz‐Boutigue, Marie‐Hélène

doi:10.1007/s00018-004-4461-9

Cited by 118 publications

(187 citation statements)

References 50 publications

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“…[ 52 ] They showed that bovine cateslytin (in the low micromolar range) readily crosses the cell membrane of fungi and rapidly reaches the intracellular space. Then the growth was arrested as soon as the peptide penetrated into the developing fungus.…”

Section: Bacterial Assaysmentioning

confidence: 99%

Harnessing the Multifunctionality in Nature: A Bioactive Agent Release System with Self‐Antimicrobial and Immunomodulatory Properties

Özçelik

Vrana

Gudima

et al. 2015

Adv Healthcare Materials

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Major problems with biomedical devices in particular implants located in nonsterile environments concern: (i) excessive immune response to the implant, (ii) development of bacterial biofi lms, and (iii) yeast and fungi infections. An original multifunctional coating that addresses all these issues concomitantly is developed. A new exponentially growing polyelectrolyte multilayer fi lm based on polyarginine (PAR) and hyaluronic acid (HA) is designed. The fi lms have a strong inhibitory effect on the production of infl ammatory cytokines released by human primary macrophage subpopulations. This could reduce potential chronic infl ammatory reaction following implantation. Next, it is shown that PAR, due to its positive charges, has an antimicrobial activity in fi lm format against Staphylococcus aureus for 24 h. In order to have a long-term antimicrobial activity, a precursor nanoscale silver coating is deposited on the surface before adding the PAR/HA fi lms. Moreover, the PAR/HA fi lms can be easily further functionalized by embedding antimicrobial peptides, like catestatin (CAT), a natural host defense peptide. This PAR/HA+CAT fi lm proves to be effective as an antimicrobial coating against yeast and fungi and its cytocompatibility is also assessed. Finally, this all-in-one system constitutes an original strategy to limit infl ammation and prevents bacteria, yeast, and fungi infections.

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Section: Bacterial Assaysmentioning

confidence: 99%

Harnessing the Multifunctionality in Nature: A Bioactive Agent Release System with Self‐Antimicrobial and Immunomodulatory Properties

Özçelik

Vrana

Gudima

et al. 2015

Adv Healthcare Materials

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“…catestatin acts to stabilize baseline conditions in several in vivo and in vitro models (2,5,15,30,34,48). As such, the role of catestatin may not be apparent until an appropriate stimulus is applied.…”

Section: R723 Pacap Is Hypotensive In the Presence Of Catestatinmentioning

confidence: 99%

Catestatin has an unexpected effect on the intrathecal actions of PACAP dramatically reducing blood pressure

Gaede

Inglott

Farnham

et al. 2012

American Journal of Physiology-Regulatory, Integrative and Comp

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Gaede AH, Inglott MA, Farnham MM, Pilowsky PM. Catestatin has an unexpected effect on the intrathecal actions of PACAP dramatically reducing blood pressure. Am J Physiol Regul Integr Comp Physiol 303: R719 -R726, 2012. First published August 8, 2012 doi:10.1152/ajpregu.00202.2012.-This study focuses on presympathetic neurons of the rostral ventrolateral medulla (RVLM) that regulate sympathetic vasomotor tone. Many neurotransmitters are colocalized in RVLM neurons and are released under specific conditions to modulate efferent homeostatic responses. Of particular interest here are two peptides colocalized in catecholaminergic RVLM neurons: catestatin and pituitary adenylate cyclase-activating polypeptide (PACAP). Chromogranin A-derived catestatin is a potent endogenous noncompetitive nicotinic and adrenoreceptor antagonist. Catestatin impairs adenylate cyclase and phospholipase C action: mechanisms engaged by PACAP. Although PACAP and catestatin are likely coreleased, the possible effects of this are unknown. We aimed to determine whether catestatin affects the normal sympathoexcitatory but isotensive responses to intrathecal PACAP. Urethane-anesthetized, vagotomized, ventilated Sprague-Dawley rats (n ϭ 22) were given an intrathecal injection of catestatin at different times prior to intrathecal administration of PACAP-38. Arterial pressure, splanchnic sympathetic nerve activity, heart rate, and reflex responses to baroreceptor and chemoreceptor activation were recorded. The key findings of this study are that pretreatment with catestatin time dependently enhances the PACAP-38 effect on mean arterial pressure and enhances sympathetic barosensitivity and chemosensitivity. The timescale of the effect of catestatin on the response to PACAP-38 strongly suggests that catestatin is either causing changes in gene expression to exert its effects, or modifying intracellular mechanisms normally engaged by PAC 1 receptors. The ability of catestatin pretreatment to enhance barosensitivity and chemosensitivity after PACAP-38 injection supports the hypothesis that catestatin manipulates the intracellular environment within sympathetic neurons in a way that increases responses to PACAP. chromogranin a; sympathetic; baroreflex; chemoreflex; epinephrine HYPERTENSION IS A MAJOR HUMAN health problem that is due, in large part, to increased sympathetic drive that emanates from the rostral ventrolateral medulla (RVLM) (42,44,45). Central neural mechanisms maintain arterial blood pressure by integrating information from reflexes and central behavioral and emotional states to alter efferent sympathetic and parasympathetic activity, according to need (18,44,45). The RVLM plays a critical role in this system as the final integrative pathway in the regulation of sympathetic tone and cardiovascular adaptive reflexes (e.g., the baroreceptor and chemoreceptor reflexes) (45). The RVLM contains a functionally heterogeneous cell population that includes barosensitive, presympathetic (C1) neurons, which project to sympathetic preganglionic neu...

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“…A series of studies on the antimicrobial function of the CGA-derived peptides has been conducted (19)(20)(21)(22). Catestatin (derived from the C-terminus of bovine CGA) is expressed in murine skin in response to injury and infection, and it has a potent antimicrobial activity against bacteria, fungi and yeast.…”

Section: Introductionmentioning

confidence: 99%

“…Similar activity has also been reported for vasostatin-I (CGA1-76; corresponding to CGA residues 1-76), which can trigger the selective migration of human monocytes and eosinophils. CGA1-76 and catestatin have been described to be innate immunity components of mammals (19,21). A study by Lugardon et al (19) showed that the C-terminal moiety of bovine CGA1-76 exhibited a potent antifungal activity and that the disulfide-bridge loop Cys 17 -Cys 38 was crucial for its antibacterial activity, but not for the antifungal activity.…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative effect and characterization of a novel antifungal peptide derived from human Chromogranin A

Zhang

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. CGA-N46 is a novel antifungal peptide derived from the N-terminus of human Chromogranin A, corresponding to the 31st to 76th amino acids. Further research on its activities and characteristics may be helpful for the application of CGA-N46 in medical or other situations. In the present study, the antifungal spectrum and physicochemical characteristics of CGA-N46 were investigated using an antifungal assay, its antiproliferative effects on cancer and normal cells were assessed using MTT assay and its combinatorial effect with other antibiotics was analyzed using checkerboard analysis. The results showed that CGA-N46 exhibited antifungal activity against the tested Candidas (C. glabrata, C. parapsilosis, C. krusei, C. tropicalis and C. albicans) at a concentration of <0.8 mM, but had no effect on the growth of filamentous fungi or other types of fungi (Cryptococcus neoformans, Aspergillus f umigatus, Aspergillus f lavus, Aspergillus niger, Fusarium moniliforme, Microsporum canis, Microsporum gypseum, Trichophyton rubrum and Trichophyton mentagrophytes), even at a concentration of 3.2 mM. CGA-N46 had an inhibitory effect on the proliferation of lung cancer A549 cells and a reversible effect on the growth of normal primary chicken embryo fibroblast cells, but no hemolytic activity on human erythrocytes at the minimum inhibitory concentration of CGA-N46 against yeasts. The antifungal activity of CGA-N46 was stable at a temperature <40˚C or within a broad pH range (pH 5.0-7.0). Its antifungal activity was enhanced when the peptide was used in combination with fluconazole and terbinafine. The present results indicate that CGA-N46 is a safe, physicochemically stable, antifungal peptide with anticancer cell activity that exhibits an additive effect with conventional antibiotics.

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New antimicrobial activity for the catecholamine release-inhibitory peptide from chromogranin A

Cited by 118 publications

References 50 publications

Harnessing the Multifunctionality in Nature: A Bioactive Agent Release System with Self‐Antimicrobial and Immunomodulatory Properties

Harnessing the Multifunctionality in Nature: A Bioactive Agent Release System with Self‐Antimicrobial and Immunomodulatory Properties

Catestatin has an unexpected effect on the intrathecal actions of PACAP dramatically reducing blood pressure

Antiproliferative effect and characterization of a novel antifungal peptide derived from human Chromogranin A

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