New Antituberculosis Drug FS-1

Islamov, Rinat; Kerimzhanova, Bahkytzhan; Ilin, Alexander

doi:10.5772/intechopen.80795

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…The multidrug-resistant strain A. baumannii ATCC BAA-1790 was isolated from sputum in 2008 in Washington, DC. In this study, it is used as a model organism to investigate genomic and population changes under the effect of a new drug, FS-1, which induces the reversion of multidrug-resistant bacteria to their antibiotic-sensitive phenotypes (1,2).…”

mentioning

confidence: 99%

Complete Genome Sequence of Collection Strain Acinetobacter baumannii ATCC BAA-1790, Used as a Model To Study the Antibiotic Resistance Reversion Induced by Iodine-Containing Complexes

Korotetskiy¹,

Joubert

Magabotha

et al. 2020

Microbiol Resour Announc

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mentioning

confidence: 99%

Complete Genome Sequence of Collection Strain Acinetobacter baumannii ATCC BAA-1790, Used as a Model To Study the Antibiotic Resistance Reversion Induced by Iodine-Containing Complexes

Korotetskiy¹,

Joubert

Magabotha

et al. 2020

Microbiol Resour Announc

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Clinical Trials on Novel Advanced Drugs for Chronic Respiratory Disorders

Mudnakudu-Nagaraju

Mohan

Chandrashekarappa

et al. 2022

Advanced Drug Delivery Strategies for Targeting Chronic Inflammatory Lung Diseases

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Transcriptomics and methylomics study on the effect of iodine-containing drug FS-1 onEscherichia coliATCC BAA-196

Korotetskiy¹,

Jumagaziyeva²,

Shilov³

et al. 2020

Preprint

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21 Recent studies have demonstrated that antibiotic resistance in pathogenic bacteria could be 22 reverted to the drug sensitive phenotype by the treatment with the newly introduced iodine 23 containing nanomolecular complex, FS-1. Antibiotic resistance reversion has been verified as a 24 promising therapeutic approach to combat multidrug resistant infections. The mechanisms of 25 action, however, remain unclear. A collection strain, Escherichia coli ATCC showing 26 an extended spectrum of resistance to beta-lactam and aminoglycoside antibiotics was used in 27 this study as a model organism. The FS-1 treated culture and the negative control variant were 28 sequenced by PacBio RS II System following the SMRTbell 20-kb library preparation protocol.29 Total RNA samples of these strains were sequenced by Ion Torrent. It was shown that the 30 treatment with FS-1 caused a profound gene expression alternation switching the bacterial 31 metabolism to anaerobic respiration, increased anabolism, oxidative/acidic stress response and an 32 inhibition of many nutrient uptake systems. All this leads to an increase in the susceptibility to 33 antibiotics even when FS-1 is removed from the medium. The later fact implies an involvement 34 of epigenetic mechanisms in gene regulation and antibiotic resistance reversion. This hypothesis 35 was investigated by base-call kinetic analysis in PacBio reads and DNA methylation profiling in 36 the sequenced genomes. Several DNA motifs of adenosine and cytosine methylation were 37 identified. While the numbers of methylated sites in chromosomes and plasmids of both 38 genomes, NC and FS, were similar, the distribution of the methylated sites was different. It may 39 explain the observed long lasting effect of the treatment of E. coli with FS-1 on antibiotic 40 susceptibility of this model microorganism.41 Author summary 42 The emergence of multidrug resistant bacteria is a great concern, since the misuse of antibiotics 43 have caused a strong selective pressure for these resistant bacteria and various treatment options 44 are becoming ineffective. Drug induced reversion of antibiotic resistant is considering a 45 promising approach to address this problem. This study was set out to investigate genetic 46 mechanisms of action of a new iodine-containing nano-micelle drug FS-1 that induces antibiotic 47 resistance reversion in bacteria. Escherichia coli ATCC BAA-196 was used as a model of 48 multidrug resistant microorganisms. With this purpose in mind, we have sequenced the genomic 49 DNA and total RNA samples of E. coli cultivated on medium containing FS-1, served as an 50 experimental (FS) variant, and bacteria cultivated on normal medium, served as negative control 51 (NC). RNA sequencing showed a differential gene expression in the FS-1 treated strain that may 52 explain the observed increase in susceptibility to gentamicin and ampicillin. Application of the 53 3 rd generation sequencing technology, SMRT PacBio, allowed an unambiguous whole genome 54 assembly of NC and FS variants, a...

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New Antituberculosis Drug FS-1

Cited by 3 publications

References 43 publications

Complete Genome Sequence of Collection Strain Acinetobacter baumannii ATCC BAA-1790, Used as a Model To Study the Antibiotic Resistance Reversion Induced by Iodine-Containing Complexes

Complete Genome Sequence of Collection Strain Acinetobacter baumannii ATCC BAA-1790, Used as a Model To Study the Antibiotic Resistance Reversion Induced by Iodine-Containing Complexes

Clinical Trials on Novel Advanced Drugs for Chronic Respiratory Disorders

Transcriptomics and methylomics study on the effect of iodine-containing drug FS-1 onEscherichia coliATCC BAA-196

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