New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species

Reut, V. E.; Kozlov, Stanislav; Kudryavtsev, I. V.; Грудинина, Н. А.; Kostevich, Valeria A.; Горбунов, Н. П.; Grigorieva, Daria V.; Kalvinkovskaya, Ju. A.; Бушук, С. Б.; Varfolomeeva, E. Yu.; Fedorova, Natalia D.; Gorudko, Irina V.; Панасенко, О. М.; Vasilyev, V. B.; Sokolov, A. V.

doi:10.3390/antiox11091719

Cited by 4 publications

(2 citation statements)

References 62 publications

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“…MPO chlorinating activity was evaluated fluorometrically using Celestine Blue B (CB) which was oxidized to fluorescent glycol (λ ex = 487 nm, λ em = 578 nm) by taurine N-chloramine produced in the reaction of taurine with HOCl generated by MPO [ 39 ]. The assay was performed in 96-plate format.…”

Section: Methodsmentioning

confidence: 99%

Effects of Synthetic Short Cationic Antimicrobial Peptides on the Catalytic Activity of Myeloperoxidase, Reducing Its Oxidative Capacity

et al. 2022

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Cationic antimicrobial peptides (CAMPs) have gained attention as promising antimicrobial therapeutics causing lower or no bacterial resistance. Considerable achievements have been made in designing new CAMPs that are highly active as antimicrobials. However, there is a lack of research on their interaction with biologically important proteins. This study focused on CAMPs’ effects on myeloperoxidase (MPO), an enzyme which is microbicidal and concomitantly damaging to host biomolecules and cells due to its ability to produce reactive oxygen and halogen species (ROS/RHS). Four CAMPs designed by us were employed. MPO catalytic activity was assessed by an absorbance spectra analysis and by measuring enzymatic activity using Amplex Red- and Celestine Blue B-based assays. The peptide Hm-AMP2 accelerated MPO turnover. Pept_1545 and Hm-AMP8 inhibited both the MPO chlorinating and peroxidase activities, with components of different inhibition types. Hm-AMP8 was a stronger inhibitor. Its Ki towards H2O2 and Cl– was 0.3–0.4 μM vs. 11–20 μM for pept_1545. Peptide tyrosine and cysteine residues were involved in the mechanisms of the observed effects. The results propose a possible dual role of CAMPs as both antimicrobial agents and agents that downregulate MPO activation, and suggest CAMPs as prototypes for the development of antioxidant compounds to prevent MPO-mediated ROS/RHS overproduction.

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Section: Methodsmentioning

confidence: 99%

Effects of Synthetic Short Cationic Antimicrobial Peptides on the Catalytic Activity of Myeloperoxidase, Reducing Its Oxidative Capacity

et al. 2022

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“…Celestine blue dye is used with iron-aluminium complexes as a substitute for haematoxylin in H-E (haematoxylin-eosin) staining because of its resistance to low-pH solutions. It has been used as a new electroactive indicator in DNA biosensors and is also applicable to HOCl detection in living cells and to assaying the chlorinating activity of myeloperoxidase [40]. In microbiology, neutral red is used in the MacConkey agar to differentiate bacteria for lactose fermentation [36].…”

Section: Pitucamycinmentioning

confidence: 99%

Diversity-Orientated Synthesis and Biological Properties of Compounds Based on the N-Phenylquinoneimine Scaffold

Adesina,

Skouta

2024

Molecules

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The N-phenylquinoneimine scaffold is a versatile synthetic platform that has gained significant attention in the field of drug discovery due to its structural diversity and capacity to interact with biologically relevant targets. This review explores established synthetic methodologies and highlights the significant biological activities exhibited by compounds derived from this scaffold, their implications for medicinal chemistry, and the development of novel therapeutics.

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Ex Tenebris Lux: Illuminating Reactive Oxygen and Nitrogen Species with Small Molecule Probes

Cabello,

Chen,

Melville

et al. 2024

Chem. Rev.

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Reactive oxygen and nitrogen species are small reactive molecules derived from elements in the airoxygen and nitrogen. They are produced in biological systems to mediate fundamental aspects of cellular signaling but must be very tightly balanced to prevent indiscriminate damage to biological molecules. Small molecule probes can transmute the specific nature of each reactive oxygen and nitrogen species into an observable luminescent signal (or even an acoustic wave) to offer sensitive and selective imaging in living cells and whole animals. This review focuses specifically on small molecule probes for superoxide, hydrogen peroxide, hypochlorite, nitric oxide, and peroxynitrite that provide a luminescent or photoacoustic signal. Important background information on general photophysical phenomena, common probe designs, mechanisms, and imaging modalities will be provided, and then, probes for each analyte will be thoroughly evaluated. A discussion of the successes of the field will be presented, followed by recommendations for improvement and a future outlook of emerging trends. Our objectives are to provide an informative, useful, and thorough field guide to small molecule probes for reactive oxygen and nitrogen species as well as important context to compare the ecosystem of chemistries and molecular scaffolds that has manifested within the field.

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New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species

Cited by 4 publications

References 62 publications

Effects of Synthetic Short Cationic Antimicrobial Peptides on the Catalytic Activity of Myeloperoxidase, Reducing Its Oxidative Capacity

Effects of Synthetic Short Cationic Antimicrobial Peptides on the Catalytic Activity of Myeloperoxidase, Reducing Its Oxidative Capacity

Diversity-Orientated Synthesis and Biological Properties of Compounds Based on the N-Phenylquinoneimine Scaffold

Ex Tenebris Lux: Illuminating Reactive Oxygen and Nitrogen Species with Small Molecule Probes

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