New Approach for Specific Determination of Antibiotics by Use of Luminescent
            <i>Escherichia coli</i>
            and Immune Serum

Vlasova, Irina I.; Asrieli, Tatyana V.; Gavrilova, Elisaveta M.; Danilov, V.S.

doi:10.1128/aem.70.2.1245-1248.2004

Cited by 12 publications

(6 citation statements)

References 16 publications

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“…A strain of Escherichia coli has been described that can detect low levels of tetracyclines by using an engineered strain with the luciferase gene under the control of a transposon tet operon that is induced by low levels of tetracyclines, but not other drugs, resulting in an increase in luminescence (27). On the other hand, luminescent engineered E. coli strains have been used to detect antibiotics by measuring the inhibition of luminescence (48). In both cases, one needs a series of measurements with a luminometer.…”

mentioning

confidence: 99%

Selective Advantage of Resistant Strains at Trace Levels of Antibiotics: a Simple and Ultrasensitive Color Test for Detection of Antibiotics and Genotoxic Agents

Liu¹,

Fong²,

Becket³

et al. 2011

Antimicrob Agents Chemother

144

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Many studies have examined the evolution of bacterial mutants that are resistant to specific antibiotics, and many of these focus on concentrations at and above the MIC. Here we ask for the minimum concentration at which existing resistant mutants can outgrow sensitive wild-type strains in competition experiments at antibiotic levels significantly below the MIC, and we define a minimum selective concentration (MSC) in Escherichia coli for two antibiotics, which is near 1/5 of the MIC for ciprofloxacin and 1/20 of the MIC for tetracycline. Because of the prevalence of resistant mutants already in the human microbiome, allowable levels of antibiotics to which we are exposed should be below the MSC. Since this concentration often corresponds to low or trace levels of antibiotics, it is helpful to have simple tests to detect such trace levels. We describe a simple ultrasensitive test for detecting the presence of antibiotics and genotoxic agents. The test is based on the use of chromogenic proteins as color markers and the use of single and multiple mutants of Escherichia coli that have greatly increased sensitivity to either a wide range of antibiotics or specific antibiotics, antibiotic families, and genotoxic agents. This test can detect ciprofloxacin at 1/75 of the MIC.

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mentioning

confidence: 99%

Selective Advantage of Resistant Strains at Trace Levels of Antibiotics: a Simple and Ultrasensitive Color Test for Detection of Antibiotics and Genotoxic Agents

Liu¹,

Fong²,

Becket³

et al. 2011

Antimicrob Agents Chemother

144

View full text Add to dashboard Cite

show abstract

“…Sensitivity of the bacteria to antibiotics increased after the incubation of cells in a human serum solution. The highest sensitivity was observed for aminoglycoside antibiotics (gentamycin and streptomycin) with a lower LOD of 2.8 ng/mL [16].…”

Section: Medical Products and Other Compoundsmentioning

confidence: 98%

Biosensor development in Russia

Reshetilov

2007

Biotechnology Journal

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The review summarizes the current Russian research in the field of biological sensors for detection of carbohydrates, alcohols, medicines, enzyme inhibitors, toxicants, heavy metal ions, as well as viruses and microbial cells. Some of the presented works describe the analytical parameters of biosensors; other publications provide a basis for their development. The review covers mainly publications that have appeared over the past 10 years. As a whole, the collected material gives an idea of the main tendencies of biosensor development in Russia. The review is not meant to be comprehensive but highlights the major trends in this field in the last decade.

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“…Antibiotics inhibited luminescence of a genetically engineered bacteria strain; the system sensitivity to some antibiotics grew notably after the cells had been preactivated by blood serum. (118) To date, a variety of antibiotics in milk or other animal foods, including β-lactam, penicillin, tetracycline, and macrolides, have been detected with microbial biosensors. Downloaded by [University of Calgary] at 07: 23 18 September 2013 In addition, microbial sensors have been developed to measure several other components in food, including iron (T. ferrooxidans), ammonia (B. subtilis), acetic acid (Trichosporon brassicae), hydrogen peroxide (B. subtilis), pyruvate, phosphate, peptide sweetener/aspartame, fish spoilage, and so on.…”

Section: Microbial Biosensors For Food Analysismentioning

confidence: 99%

Microbial Biosensors for Environmental Monitoring and Food Analysis

Ying

2011

Food Reviews International

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A microbial biosensor is an elaborate analytic device that uses microorganisms as recognition elements, mainly for application in environmental monitoring, food safety, military defense, and medicine. The selection and immobilization of microorganisms are key steps that must first be addressed for microbial biosensors. Currently, genetically modified microorganisms play an increasingly significant role in improving the capacity of biosensors. Electrochemical and optical types of transducers have been widely employed in microbial biosensors, although bioluminescence and fluorescence methods have been highlighted recently. Additionally, the microbial fuel cell (MFC), which has been mainly applied in biological oxygen demand (BOD) biosensors, is a promising technology. This article reviews recent developments of microbial biosensors with respect to their applications in environmental monitoring and food analysis, including measurement of a variety of common pollutants, products in fermenting processes, antibiotic residues, and toxins in food. Current limitations and prospective future directions, such as performance optimization, developing portable biosensors for on-site monitoring, combination of genetic and DNA approaches, nanotechnology, and phage-based biosensors for foodborne pathogens, are also discussed in this review.

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New Approach for Specific Determination of Antibiotics by Use of Luminescent Escherichia coli and Immune Serum

Cited by 12 publications

References 16 publications

Selective Advantage of Resistant Strains at Trace Levels of Antibiotics: a Simple and Ultrasensitive Color Test for Detection of Antibiotics and Genotoxic Agents

Selective Advantage of Resistant Strains at Trace Levels of Antibiotics: a Simple and Ultrasensitive Color Test for Detection of Antibiotics and Genotoxic Agents

Biosensor development in Russia

Microbial Biosensors for Environmental Monitoring and Food Analysis

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