New approach for the detection of non-ribosomal peptide synthetase genes in Bacillus strains by polymerase chain reaction

Tapi, Arthur; Chollet-Imbert, Marlène; Scherens, Bart; Jacques, Philippe

doi:10.1007/s00253-009-2176-4

Cited by 92 publications

(74 citation statements)

References 43 publications

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“…A universal PCR assay targeting the surfactin class of nonribosomal peptide synthetases (NRPS), developed by Tapi et al (69), was performed to elucidate the nonribosomal peptide synthesis capabilities of the obtained hemolytic Bacillus spp. isolates.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Airborne Bacteria at an Underground Subway Station

Dybwad

Granum

Bruheim

et al. 2012

Appl Environ Microbiol

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The reliable detection of airborne biological threat agents depends on several factors, including the performance criteria of the detector and its operational environment. One step in improving the detector's performance is to increase our knowledge of the biological aerosol background in potential operational environments. Subway stations are enclosed public environments, which may be regarded as potential targets for incidents involving biological threat agents. In this study, the airborne bacterial community at a subway station in Norway was characterized (concentration level, diversity, and virulence-and survival-associated properties). In addition, a SASS 3100 high-volume air sampler and a matrix-assisted laser desorption ionization-time of flight mass spectrometry-based isolate screening procedure was used for these studies. The daytime level of airborne bacteria at the station was higher than the nighttime and outdoor levels, and the relative bacterial spore number was higher in outdoor air than at the station. The bacterial content, particle concentration, and size distribution were stable within each environment throughout the study (May to September 2010). The majority of the airborne bacteria belonged to the genera Bacillus, Micrococcus, and Staphylococcus, but a total of 37 different genera were identified in the air. These results suggest that anthropogenic sources are major contributors to airborne bacteria at subway stations and that such airborne communities could harbor virulence-and survival-associated properties of potential relevance for biological detection and surveillance, as well as for public health. Our findings also contribute to the development of realistic testing and evaluation schemes for biological detection/surveillance systems by providing information that can be used to mimic real-life operational airborne environments in controlled aerosol test chambers.

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Section: Methodsmentioning

confidence: 99%

Characterization of Airborne Bacteria at an Underground Subway Station

Dybwad

Granum

Bruheim

et al. 2012

Appl Environ Microbiol

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“…Additionally, to confi rm the presence of genes encoding enzymes involved in LP synthesis in T-1, T′-1, and I′-1a Bacillus strains, the polymerase chain reaction with primer pairs proposed by Tapi et al ( 2010 ) and Hsieh et al ( 2004 ) were used (Fig. 1 ).…”

Section: Methods For Detection Of Biosurfactant-producing Bacillus Stmentioning

confidence: 99%

Characterization of Bacillus Strains Producing Biosurfactants

Płaza

Pacwa-Płociniczak

Piotrowska‐Seget

et al. 2014

Environmental Sustainability

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Genus Bacillus includes species of industrial, biotechnological, and environmental interest, as well as clinically important strains. In terms of metabolic properties, they present a diverse group, as they can degrade various substrates and produce many molecules, including lipopeptide (LP) biosurfactants. Due to a high interest in biosurfactants for application in different fi elds, the molecular mechanisms of regulation of the expression of the operons responsible for LPs have been intensively studied. Additionally, many assays have been created to evaluate the use of cost-effective renewable agro-industrial substrates for production. The purpose of the chapter is to provide a comprehensive overview of the results of our studies on identifi cation, characterization, and assessment ability of three Bacillus strains to produce biosurfactants and detection of genes encoding enzymes involved in biosurfactant synthesis. Moreover, the use of alternative substrates to decrease the cost of LP biosurfactant production and some aspects of application of Bacillus spp. as biocontrol agents are discussed.

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“…Several different detection methods for lichenysin have been developed including cytotoxicity methods like the sperm motility assay (Andersson et al 1998, Hoornstra et al 2003) and the Vero cell assay (Sandvig and Olsnes 1982), and PCR A c c e p t e d M a n u s c r i p t (Madslien et al, 2013, Nieminen et al, 2007, Tapi et al 2010, Turgay and Marahiel 1994, Wu et al 2015. None of these methods proves the presence of lichenysin, only a possible effect of the lipopeptide or the genes encoding lichenysin synthetase.…”

Section: Introductionmentioning

confidence: 99%