New approach to cross-polarized optical coherence tomography based on orthogonal arbitrarily polarized modes

Геликонов, Г. В.; Gelikonov, Valentin M.

doi:10.1117/12.704015

Cited by 9 publications

(10 citation statements)

References 27 publications

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“…Multimodal optical coherence tomography (OCT) is a non-invasive method for obtaining images of biological tissues with a high spatial resolution (10-15 μm) up to a depth of 1.5 mm [14,15]. Using OCT, one can evaluate both the general structure of biological tissue (number and thickness of its layers), the state of the connective tissue, and microcirculation (blood flow and lymph flow) in real time.…”

Section: Introductionmentioning

confidence: 99%

Multimodal Optical Coherence Tomography: Imaging of Blood and Lymphatic Vessels of the Vulva

Sirotkina¹,

Potapov²,

Vagapova³

et al. 2019

Sovrem Tehnol Med

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The aim of the study was to test the method of optical coherence tomography (OCT) for its ability to obtain images of blood and lymphatic vessels of the vulva.Materials and Methods. The study was performed using a multimodal optical coherence tomography device developed in the Institute of Applied Physics of the Russian Academy of Sciences (Nizhny Novgorod). In this setup, 3D images of 3.4×3.4×1.25 mm are created within 26 s. OCT angiography and OCT lymphangiography are based on the speckle structure analysis. Visualization of blood and lymphatic vessels does not require any additional contrast agents.A histological study of vulvar biopsy samples from two locations was performed in 3 patients without vulvar pathology and in 5 patients with vulvar lichen sclerosus; the results were then compared with 3D OCT images obtained at the same locations.Results. Using the multimodal OCT, we found that normal mucous membrane of the vulva had a well-developed network of blood and lymphatic vessels; their diameters were increasing with depth. In the subepithelial zone, only blood capillary loops could be seen, whereas lymphatic capillaries became detectable starting from a depth of 170 μm (submucosa layer).In the case of vulvar lichen sclerosus, the density of the blood vessel network decreases dramatically and the capillary loops in the subepithelial zone disappear. Lymphatic vessels become detectable at a depth of 300 μm, and only few thin vessels can be detected at depths of 300 to 600 μm. The results suggest that the presentation of blood and lymphatic vessels depends on the state of the vulvar connective tissue. Specifically, the number of blood and lymphatic vessels is reduced in areas of hyalinosis and sclerosis of collagen fibers.Conclusion. For the first time, using multimodal OCT, an in vivo study of blood and lymphatic vessels of the vulvar mucosa was performed on normal and lichen sclerosus-affected vulva; the obtained OCT images were then compared with histological images of the same tissues. The OCT method allows one to diagnose a zone of hyalinosis and sclerosis of collagen fibers characterized by an abnormally low number of blood and lymphatic vessels.

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Section: Introductionmentioning

confidence: 99%

Multimodal Optical Coherence Tomography: Imaging of Blood and Lymphatic Vessels of the Vulva

Sirotkina¹,

Potapov²,

Vagapova³

et al. 2019

Sovrem Tehnol Med

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“…Each OCT image has the following characteristics: 200x200 pixel, size 2x2 mm, depth resolution in free space 15 µm, and longitudinal resolution of 25 µm registered for 2 s. The device has two channels and demonstrates two conjugate images simultaneously: in direct polarization (the image at the bottom) and in orthogonal polarization (the image on top) with strict mutual correspondence of spatial arrangement of the image elements. The device is capable of acquiring OCT images of the object in direct and orthogonal polarizations simultaneously [6]. The OCT system has an endoscopic forward-looking probe (2.7 mm in diameter).…”

Section: Methodsmentioning

confidence: 99%

Cross-polarization endoscopic optical coherence tomography in urology

Zagaynova

Gladkova

Gelikonov³

et al. 2009

SPIE Proceedings

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We present results obtained using a two-channel portable device for CP OCT based on the polarization-maintaining fiber. The device is capable of acquiring OCT images of the object in direct and orthogonal polarizations simultaneously.The OCT system has an endoscopic forward-looking probe (2.7 mm in diameter). The CP OCT imaging was done in 64 postoperative specimens, for 30 patients during cystoscopy with suspicion of urothelial neoplasia, and for 11 patients with prostatectomy due to cancer. After surgery in the tissue specimen we immediately introduced surgical suture and investigated a bladder wall by CP OCT. When comparing OCT images and histology slides we can distinguish tissue layers based on position of the suture material. CP OCT images were compared with histological data. Special stainingSirius Red for collagen types was applied. OCT -visualization of sympathetic nerve was made during open surgery.We found, that a strong signal in orthogonal polarization is produced by structures consisting of Type I collagen. We demonstrated that endoscopic benign and early malignant pathological processes that cannot be detected by standard OCT may be differentiated by CP OCT.Sympathetic nerve of neurovascular bundle gives a strong signal in both polarizations: direct and orthogonal. Based on these CP OCT features we can visualize nerve during operation (open or endoscopic surgery) and thus preserve it.

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“…Time-domain device "Polarization-sensitive optical coherence tomograph OCT-1300U" (BioMedTech LLC, Nizhny Novgorod, Russia) ( Fig. 1a), that provides two image acquisition in coand cross-polarizations was used in the study [17,21]. The device approved for clinical use (product license №FСР 2012/13479 of 30 May 2012) and has replaceable endoscopic probe (Fig.…”

Section: Cp Oct Study and Image Analysismentioning

confidence: 99%

“…CP OCT is a functional extension of OCT that enables the detection of changes in the state of polarization of light caused by birefringence and coupling between two polarization states due to scattering in the random media (cross-scattering) [17]. As a result, two types of images are obtained simultaneously: in the initial (co-) polarization and orthogonal (cross-) polarization, which allow assessing isotropic (cells) and anisotropic (collagen and elastic fibers of connective tissue) structures separately [18,19].…”

Section: Introductionmentioning

confidence: 99%

New Findings in the Study of the Pathogenesis of Urethral Pain Syndrome

Streltsova¹,

Kuyarov²,

Molvi³

et al. 2020

Preprint

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Urethral pain syndrome (UPS) is still a pathology in which the diagnosis is formulated as a "diagnosis of exclusion". The exact pathogenetic mechanisms are not yet fully understood and clear recommendations for the prevention and treatment of UPS are absent. The goal of the study was to assess the condition of the tissues in the female urethra in UPS, by using transvaginal ultrasound (TVUS) and cross-polarization optical tomography (CP OCT). TVUS showed an expansion in the diameter of the internal lumen of the urethra, especially in the proximal region compared with the norm. Compression elastography revealed areas with increased stiffness (presence of fibrosis) in urethral and surrounding tissues. When studied with CP OCT it was shown that with UPS, the structure of the tissues in most cases was changed: trophic alterations in the epithelium (hypertrophy or atrophy) and fibrosis of underlying connective tissue were observed. The proximal fragment of the urethra with UPS underwent changes identical to those of the bladder neck. This paper showed that the introduction of new technology — CP OCT — in conjunction with TVUS will allow verification of structural changes in tissues of the lower urinary tract at the level of their architectonics and will help doctors understand better the basics of the UPS pathogenesis.

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New approach to cross-polarized optical coherence tomography based on orthogonal arbitrarily polarized modes

Cited by 9 publications

References 27 publications

Multimodal Optical Coherence Tomography: Imaging of Blood and Lymphatic Vessels of the Vulva

Multimodal Optical Coherence Tomography: Imaging of Blood and Lymphatic Vessels of the Vulva

Cross-polarization endoscopic optical coherence tomography in urology

New Findings in the Study of the Pathogenesis of Urethral Pain Syndrome

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