New approach to distinguishing chemoattractants, chemorepellents and catabolised chemoeffectors for Campylobacter jejuni

Diverse Sensory Repertoire of Paralogous Chemoreceptors Tlp2, Tlp3, and Tlp4 in Campylobacter jejuni

“…Migration toward mucin, used as a control, was not affected by mutations. Results of the nutrient depletion assay were confirmed by the modified tHAP chemotaxis assay ( 34 ) (see Fig. S8 and Table S5).…”

“…Chemotaxis assays—the nutrient-depleted chemotaxis assay and the modified hard agar plug assay (tHAP)—were performed as previously described ( 11 , 34 ). The chemotactic responses of all isogenic strains were compared to that of the C. jejuni 11168-O wild-type strain to distinguish between chemoattractants and chemorepellents.…”

A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp.

“…A number of modified HAP assays have been trialed in order to qualitatively and quantitatively assess the changes in campylobacterial chemotactic behaviour [13,14,[26][27][28][29]. For instance, Du, et al [30] used a modified HAP assay to investigate the role of Cj0371 in chemotaxis.…”

Chemotactic behavior of Campylobacter fetus subspecies towards cervical mucus, bovine placenta and selected substances and ion

“…Chemotactic assays were performed using the disk method on soft agar (Vegge et al, 2009) modified by Tareen et al, (2010) as follows. C. fetus strains were grown on blood agar (Hazeleger et al, 1998;Tareen et al, 2010;Burrough et al, 2012;Elgamoudi et al, 2018) at 37 ºC under microaerophilic conditions for 36 hours, suspended in PBS, adjusted spectrophotometrically (OD 600 ) to approximately 8 x 10 9 viable CFU/mL and after mixed (1:2) (v/v) with tempered (42 ºC) soft agar (0.8% bacteriological agar; Himedia, India) (Tareen et al, 2010) to obtain the test condition of 4 x 10 9 viable CFU/mL in PBSsoft (0.4%) agar (Vegge et al, 2009). The number of viable bacteria in each bacterial suspension measured by turbidimetry was confirmed, retrospectively, by the drop counting method (Miles et al, 1938).…”