New Approach To Mark Insects for Feeding and Dispersal Studies

Hagler, James R.; Cohen, Allen Carson; Bradley-Dunlop, Deborah; Enriquez, F. Javier

doi:10.1093/ee/21.1.20

Cited by 61 publications

(58 citation statements)

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“…This study represents the first time a protein has been used to mark the flow of resources in a social insect colony. Others have used protein markers and the ELISA technique to monitor the dispersal of insect herbivores, predators, and minute parasites (Hagler et al, 1992;Hagler and Durand, 1994;Hagler, 1997 a, b;Hagler and Jackson, 1998). Protein labeling of insects and the subsequent analysis for the protein marker by ELISA (or any immunoassay) represents a significant paradigm shift in the way marking studies are conducted .…”

Section: Discussionmentioning

confidence: 99%

The flow of incoming nectar through a honey bee (Apis mellifera L.) colony as revealed by a protein marker

DeGrandi-Hoffman

Hagler

2000

Insectes soc.

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The flow of incoming nectar in honeybee (Apis mellifera L.) colonies was simulated by feeding a sucrose solution labeled with a novel protein (rabbit IgG) marker and then analyzing bee and colony samples using an enzymelinked immunosorbant assay (ELISA). The labeled sucrose solution was quickly transported to food storage and brood combs. Within 2h, equal percentages of worker bees from food storage combs, nurse bees and nectar samples tested positive for the marker. Percentages of nurse bees and larvae testing positive also were equal within the first 2 h of feeding it to a colony and these percentages increased over time. Our results suggest that workers with nectar loads deposit them into cells on either food storage or brood comb with equal frequency. The labeled sucrose solution transported to the brood comb is subsequently used by nurse bees to feed larvae. How the deposition of incoming nectar in brood comb might possibly integrate the activities of foragers and nurse bees is discussed.

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Section: Discussionmentioning

confidence: 99%

The flow of incoming nectar through a honey bee (Apis mellifera L.) colony as revealed by a protein marker

DeGrandi-Hoffman

Hagler

2000

Insectes soc.

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“…The ELISA values yielded by the negative controls were virtually identical to the values yielded by the termites collected from the unmarked control treatment. Therefore, termites from each of the four treatment groups were scored positive for the rabbit IgG mark if the absorbance value was three standard deviations above that of the unmarked control treatment mean (Hagler et al, 1992). Bar charts depicting the average (±SD) ELISA absorbance values and proportion of termites positive for rabbit IgG over the course of each experiment were constructed.…”

Section: Discussionmentioning

confidence: 99%

“…Every termite was analyzed for the presence of the mark by the rabbit IgG specific double antibody sandwich ELISA described by Hagler et al (1992). Each well of a 96-well ELISA microplate was coated with 100 ll of anti-rabbit IgG (developed in goat) (Sigma Chemical Co., St. Louis, Missouri, USA, cat.…”

Section: Termite Marking Elisamentioning

confidence: 99%

“…They discussed the potential for marking arthropods with exogenous proteins. Hagler et al (1992) were the first to show that it is feasible to mark arthropods with a foreign protein such as rabbit immunoglobulin G (IgG) that is then identified by a sensitive protein-specific enzyme-linked immunosorbent assay (ELISA). Since then, IgG marks have been applied to study the dispersal characteristics of a wide variety of insects (DeGrandi-Hoffman and Hagler, 2000;Hagler et al, 2002;Hagler and Naranjo, 2004;Blackmer et al, 2004;Peck and McQuate, 2004;Buczkowski and Bennett, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Methods to mark termites with protein for mark–release–recapture and mark–capture type studies

et al. 2009

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Studies were conducted to investigate the feasibility of marking the southwestern desert subterranean termite, Heterotermes aureus (Snyder), with rabbit immunoglobulin G (IgG) protein for mark-release-recapture (MRR) and mark-capture type studies. Qualitative laboratory studies were conducted to determine how long reagentgrade rabbit IgG is retained on or in H. aureus that were marked either externally with a topical spray, internally by feeding them a rabbit IgG-marked food source, or both internally and externally (double marked). Marked termites were detected by an anti-rabbit IgG enzyme-linked immunosorbent assay. Data indicated that the termites retained the mark for at least 35 days, regardless of the marking procedure. A second series of laboratory studies were conducted to determine how fast H. aureus acquire the mark after feeding on cardboard bait that was either sprayed or soaked in different formulations of rabbit IgG. The IgGs tested were a highly purified and costly reagent grade IgG at 5.0 mg/ml and a less pure and less costly technical grade rabbit IgG at 1.0 mg/ml. The results showed that termites acquired both marks equally well after exposure to the soaked cardboard treatment. The advantages and limitations of protein marking termites with rabbit IgG for MRR or mark-capture termite studies are discussed.

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“…Predators that fed on marked prey were assayed by an enzyme-linked immunosorbent assay (ELISA) using the antibody complementary to the mammalian antigen. This basic technique has been used before as a physiological marker to examine insect digestion (Hagler & Cohen, 1990) and as an external marker to examine insect dispersal (Hagler et al, 1992b).…”

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confidence: 99%