New approaches for the detection of invasive fungal diseases in patients following liver transplantation—results of an observational clinical pilot study

Decker, Sebastian; Krüger, Albert; Wilk, Henryk; Grumaz, Silke; Vainshtein, Yevhen; Schmitt, Felix C. F.; Uhle, Florian; Brückner, Thomas; Zimmermann, Stefan; Mehrabi, Arianeb; Mieth, Markus; Weiss, Karl Heinz; Weigand, Markus; Hofer, Stefan; Sohn, Kai; Brenner, Thorsten

doi:10.1007/s00423-019-01769-y

Cited by 14 publications

(19 citation statements)

References 62 publications

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“…Pneumocystis jirovecii is the predominant fungus reported (~25% of the fungi detected), followed by Aspergillus species (~22%), Candida species (~16%), Cryptococcus species (~7%), Rhizopus species (~6%), Fusarium species (~4%), Alternaria species, Talaromyces marneffei and other Sordariomycetes (~3% each), Histoplasma capsulatum, other yeasts and Mucor species (~2% each), as well as some other rare fungal species (collectively~5%) (Figure 2). Excluding P. jirovecii, which is generally unculturable, out of the remaining 274 fungi detected by NGS, only 105 (~38%) were recovered by fungal culture from the same or different specimens from the respective patients [26,[29][30][31][32][33][34][35][36][37][38][39]43,45,46,49,52,54,57,59,62,64,66,67,71,73,76,77]. cies (~22%), Candida species (~16%), Cryptococcus species (~7%), Rhizopus species (~6%), Fusarium species (~4%), Alternaria species, Talaromyces marneffei and other Sordariomycetes (~3% each), Histoplasma capsulatum, other yeasts and Mucor species (~2% each), as well as some other rare fungal species (collectively ~5%) (Figure 2).…”

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

“…cies (~22%), Candida species (~16%), Cryptococcus species (~7%), Rhizopus species (~6%), Fusarium species (~4%), Alternaria species, Talaromyces marneffei and other Sordariomycetes (~3% each), Histoplasma capsulatum, other yeasts and Mucor species (~2% each), as well as some other rare fungal species (collectively ~5%) (Figure 2). Excluding P. jirovecii, which is generally unculturable, out of the remaining 274 fungi detected by NGS, only 105 (~38%) were recovered by fungal culture from the same or different specimens from the respective patients [26,[29][30][31][32][33][34][35][36][37][38][39]43,45,46,49,52,54,57,59,62,64,66,67,71,73,76,77]. The majority of P. jirovecii infections diagnosed by NGS were reported in case series [34,36,39,41,48,53,55,58,62,64,65,67,72,75].…”

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

“…Lastly, publications were searched using specific fungal names and after manual examination five extra articles were added. Therefore, in total 52 articles were included in this review [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 ].…”

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

“…It was a case of allergic bronchopulmonary mycosis where A. fumigatus and Schizophyllum commune sequences were detected in the homogenised sputum and mucoid plugs of the patient [ 26 ]. Since then, up to the time of writing, a total of 319 cases of fungal infections diagnosed by NGS were described in the literature, with some in case reports and others in case series or cohorts [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 ]. In 40 out of the 319 cases (12.5%), more than 1 fungus was detected by NGS [ 26 , ...…”

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

“…Pneumocystis jirovecii is the predominant fungus reported (~25% of the fungi detected), followed by Aspergillus species (~22%), Candida species (~16%), Cryptococcus species (~7%), Rhizopus species (~6%), Fusarium species (~4%), Alternaria species, Talaromyces marneffei and other Sordariomycetes (~3% each), Histoplasma capsulatum , other yeasts and Mucor species (~2% each), as well as some other rare fungal species (collectively ~5%) ( Figure 2 ). Excluding P. jirovecii, which is generally unculturable, out of the remaining 274 fungi detected by NGS, only 105 (~38%) were recovered by fungal culture from the same or different specimens from the respective patients [ 26 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 43 , 45 , 46 , 49 , 52 , 54 , 57 , 59 , 62 , 64 , 66 , 67 , 71 , 73 , 76 , 77 ].…”

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

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Rapid Genomic Diagnosis of Fungal Infections in the Age of Next-Generation Sequencing

Tsang

Teng

Lau

et al. 2021

JoF

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Next-generation sequencing (NGS) technologies have recently developed beyond the research realm and started to mature into clinical applications. Here, we review the current use of NGS for laboratory diagnosis of fungal infections. Since the first reported case in 2014, >300 cases of fungal infections diagnosed by NGS were described. Pneumocystis jirovecii is the predominant fungus reported, constituting ~25% of the fungi detected. In ~12.5% of the cases, more than one fungus was detected by NGS. For P. jirovecii infections diagnosed by NGS, all 91 patients suffered from pneumonia and only 1 was HIV-positive. This is very different from the general epidemiology of P. jirovecii infections, of which HIV infection is the most important risk factor. The epidemiology of Talaromyces marneffei infection diagnosed by NGS is also different from its general epidemiology, in that only 3/11 patients were HIV-positive. The major advantage of using NGS for laboratory diagnosis is that it can pick up all pathogens, particularly when initial microbiological investigations are unfruitful. When the cost of NGS is further reduced, expertise more widely available and other obstacles overcome, NGS would be a useful tool for laboratory diagnosis of fungal infections, particularly for difficult-to-grow fungi and cases with low fungal loads.

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Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

“…Pneumocystis jirovecii is the predominant fungus reported (~25% of the fungi detected), followed by Aspergillus species (~22%), Candida species (~16%), Cryptococcus species (~7%), Rhizopus species (~6%), Fusarium species (~4%), Alternaria species, Talaromyces marneffei and other Sordariomycetes (~3% each), Histoplasma capsulatum , other yeasts and Mucor species (~2% each), as well as some other rare fungal species (collectively ~5%) ( Figure 2 ). Excluding P. jirovecii, which is generally unculturable, out of the remaining 274 fungi detected by NGS, only 105 (~38%) were recovered by fungal culture from the same or different specimens from the respective patients [ 26 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 43 , 45 , 46 , 49 , 52 , 54 , 57 , 59 , 62 , 64 , 66 , 67 , 71 , 73 , 76 , 77 ].…”

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

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Rapid Genomic Diagnosis of Fungal Infections in the Age of Next-Generation Sequencing

Tsang

Teng

Lau

et al. 2021

JoF

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A 29-mRNA host-response classifier identifies bacterial infections following liver transplantation – a pilot study

Halder,

Liesenfeld,

Whitfield

et al. 2024

Langenbecks Arch Surg

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Purpose Infections are common complications in patients following liver transplantation (LTX). The early diagnosis and prognosis of these infections is an unmet medical need even when using routine biomarkers such as C-reactive protein (CRP) and procalcitonin (PCT). Therefore, new approaches are necessary. Methods In a prospective, observational pilot study, we monitored 30 consecutive patients daily between days 0 and 13 following LTX using the 29-mRNA host classifier IMX-BVN-3b that determine the likelihood of bacterial infections and viral infections. True infection status was determined using clinical adjudication. Results were compared to the accuracy of CRP and PCT for patients with and without bacterial infection due to clinical adjudication. Results Clinical adjudication confirmed bacterial infections in 10 and fungal infections in 2 patients. 20 patients stayed non-infected until day 13 post-LTX. IMX-BVN-3b bacterial scores were increased directly following LTX and decreased until day four in all patients. Bacterial IMX-BVN-3b scores detected bacterial infections in 9 out of 10 patients. PCT concentrations did not differ between patients with or without bacterial, whereas CRP was elevated in all patients with significantly higher levels in patients with bacterial infections. Conclusion The 29-mRNA host classifier IMX-BVN-3b identified bacterial infections in post-LTX patients and did so earlier than routine biomarkers. While our pilot study holds promise future studies will determine whether these classifiers may help to identify post-LTX infections earlier and improve patient management. Clinical trial notation German Clinical Trials Register: DRKS00023236, Registered 07 October 2020, https://drks.de/search/en/trial/DRKS00023236

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Soluble intercellular adhesion molecule (ICAM)-1 detects invasive fungal infections in patients following liver transplantation

Decker

Incamps²,

Wilk

et al. 2020

Biomarkers

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New approaches for the detection of invasive fungal diseases in patients following liver transplantation—results of an observational clinical pilot study

Cited by 14 publications

References 62 publications

Rapid Genomic Diagnosis of Fungal Infections in the Age of Next-Generation Sequencing

Rapid Genomic Diagnosis of Fungal Infections in the Age of Next-Generation Sequencing

A 29-mRNA host-response classifier identifies bacterial infections following liver transplantation – a pilot study

Soluble intercellular adhesion molecule (ICAM)-1 detects invasive fungal infections in patients following liver transplantation

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