New Approaches to Boar Semen Evaluation, Processing and Improvement

Šutovský, Peter

doi:10.1111/rda.12554

Cited by 44 publications

(29 citation statements)

References 104 publications

(174 reference statements)

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“…Thus, new approaches to breeding soundness evaluation in the boar (i.e. DNA fragmentation analysis and biomarkers for normal sperm phenotypes) may yield a better understanding of the underlying factors causing poor reproductive performance, thereby leading to a robust solution to the problem of summer infertility (Sutovsky 2015).…”

Section: Effect Of Heat Stress On Boar Fertilitymentioning

confidence: 99%

Revisiting summer infertility in the pig: could heat stress-induced sperm DNA damage negatively affect early embryo development?

Peña

Gummow

Parker³

et al. 2017

Anim. Prod. Sci.

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Abstract.Temperature is a crucial factor in mammalian spermatogenesis. The scrotum, pampiniform plexus, and cremaster and dartos muscles in mammals are specific adaptations to ensure sperm production in a regulated environment 4À6 C below internal body temperature. However, the limited endogenous antioxidant systems inherent in mammalian spermatozoa compounded by the loss of cytosolic repair mechanisms during spermatogenesis, make the DNA in these cells particularly vulnerable to oxidative damage. Boar sperm is likely to be more susceptible to the effects of heat stress and thus oxidative damage due to the relatively high unsaturated fatty acids in the plasma membrane, low antioxidant capacity in boar seminal plasma, and the boar's non-pendulous scrotum. Heat stress has a significant negative impact on reproductive performance in piggeries, which manifests as summer infertility and results in productivity losses that amount to millions of dollars. This problem is particularly prevalent in tropical and subtropical regions where ambient temperatures rise beyond the animal's zone of thermal comfort. Based on preliminary studies in the pig and other species, this article discusses whether heat stress could induce sufficient DNA damage in boar sperm to significantly contribute to the high rates of embryo loss and pregnancy failure observed in the sow during summer infertility. Heat stress-induced damage to sperm DNA can lead to disrupted expression of key developmental genes essential for the differentiation of early cell lineages, such as the trophectoderm, and can distort the timely formation of the blastocyst; resulting in a failure of implantation and ultimately pregnancy loss. Confirming such a link would prompt greater emphasis on boar management and strategies to mitigate summer infertility during periods of heat stress.

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Section: Effect Of Heat Stress On Boar Fertilitymentioning

confidence: 99%

Revisiting summer infertility in the pig: could heat stress-induced sperm DNA damage negatively affect early embryo development?

Peña

Gummow

Parker³

et al. 2017

Anim. Prod. Sci.

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“…In addition, HS can negatively affect testosterone, causing hypertrophy and weakening of the Leydig cell function (Stone and Seamark, 1984). The damaged DNA of sperm can increase abnormality, which could cause low fertility and subsequently could lead to reduced embryo survival (Sutovsky, 2015;Peña, et al, 2016;Zheng et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Effect of Ascorbic Acid and/or Alpha-Tocopherol Fortification on Semen Quality, Metabolic Profile, Antioxidants Status, and Dna of Roosters Exposed to Heat Stress

2020

THE JAPS

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This study investigates the influence of heat stress (HS) and vitamin C (VC) and/or vitamin E (VE) on semen quality, biochemical constituents of seminal and blood plasma, antioxidant status and jejunum and ilium deoxyribonucleic acid (DNA) of roosters. Forty-five, 32-week-old roosters were divided into five groups of nine replicates. One group was kept under a thermoneutral environment, whereas the other four were kept under HS. One of the four groups served as an unsupplemented HS group and was fed the basal diet only. The other three HS groups were supplemented with VC (200 mg/kg ascorbic acid and/or VE 150 mg/kg α-tocopherol acetate). Vitamins fortification completely restored sperm liveability , semen pH, fertility, seminal plasma total protein and globulin to the control level. Vitamins fortification completely recovered seminal plasma and blood plasma total antioxidant capacity (TAC) and malondialdehyde (MDA), red blood cell (RBCs), haemoglobin (Hgb), packed cell volume (PCV), white blood cells (WBCs), blood plasma glucose, lipid profiles, liver enzymes, and renal function. Heterophil/lymphocyte (H/L) ratio was totally restored following VE fortification. Vitamins fortification significantly increased jejunum and ileum DNA compared with the HS group, with VC inducing a complete recovery. In conclusion, either 200 mg /kg VC or 150 mg/kg VE improved semen quality, fertility, seminal plasma and blood biochemistry and haematology of HS roosters, with the VE group yielding greater immunity.

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“…In the field of male reproductive health, the role of cytometry, among other techniques for semen analysis, was reported by several authors [1][2][3]. Some elegant reviews focus on the relevant role of FCM on animal and human spermatology [4,5].…”

Section: Introductionmentioning

confidence: 99%

The Role of Cytometry for Male Fertility Assessment in Toxicology

Pereira¹,

Oliveira²,

Fonseca³

et al. 2016

Flow Cytometry - Select Topics

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Infertility is nowadays a major concern, affecting approximately 8-12% of the couples and the male factor accounts for about 50% of the cases. Occupational and/or environmental exposure to heavy metals and other pollutants is the main cause of male infertility. Lead, cadmium and chromium are heavy metals widely used in industry and quite persistent in the environment, raising major concerns over the possible effects on the reproductive health of workers and the general population. Sperm DNA integrity is essential for the accurate transmission of paternal genetic information, and normal sperm chromatin structure is important for sperm fertilizing ability. Flow cytometry can be to characterize multiple physical characteristics of the population of spermatozoa in the sperm, including sperm concentration, viability, mitochondrial mass and function, acrosome integrity, capacitation, membrane fluidity, DNA content and status, etc. This chapter elucidates the role of cytometry in the study of male fertility under toxicological insult by pollutants such as chromium, cadmium and lead. Some representative examples are presented using in vivo studies with rodents. In addition, complementary techniques to cytometry and future perspectives will be mentioned in an interdisciplinary point of view to gain knowledge on this subject.

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New Approaches to Boar Semen Evaluation, Processing and Improvement

Cited by 44 publications

References 104 publications

Revisiting summer infertility in the pig: could heat stress-induced sperm DNA damage negatively affect early embryo development?

Revisiting summer infertility in the pig: could heat stress-induced sperm DNA damage negatively affect early embryo development?

Effect of Ascorbic Acid and/or Alpha-Tocopherol Fortification on Semen Quality, Metabolic Profile, Antioxidants Status, and Dna of Roosters Exposed to Heat Stress

The Role of Cytometry for Male Fertility Assessment in Toxicology

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