New aspect of guinea pig liver 17β-hydroxysteroid (testosterone) dehydrogenase

Purification and Properties of NADP‐Dependent 17β‐Hydroxysteroid Dehydrogenase Solubilized from Porcine‐Testicular Microsomal Fraction

“…The NADP+-dependent enzyme was partially purified [38] and was separated into two fractions by ammonium sulfate precipitation following by DEAE-cellulose chromatography [39]. One of the fractions catalyzed dehydrogenations of both testosterone and 3-hydroxyhexobarbita1, the other fraction retained only 17t9-hydroxysteroid dehydrogenase activity.…”

Purification and properties of a new testosterone 17β-dehydrogenase (NADP+) from guinea-pig liver

“…Purification oftestosterone 17fi-dehydrogenase. The new testosterone 17fi-dehydrogenase was purified by a modification of the procedure for 3-hydroxyhexobarbital dehydrogenase (Kageura & Toki, 1975). All steps were carried out at 0-4°C and each enzyme fraction was concentrated in a Diaflo PM-10 filter (Amicon Corp., Lexington, MA, U.S.A.).…”

“…In a previous paper we demonstrated that testosterone 17fi-dehydrogenase (NADP+) (EC 1.1.1.64), obtained from guinea-pig liver cytosol, participates in the reversible oxidation of 3-hydroxyhexobarbital [5-(3'-hydroxy-1'-cyclohexen-1'-yl)-1,5-dimethylbarbituric acid], the microsomal oxygenation product ofhexobarbital (Kageura & Toki, 1971). This enzyme was subsequently purified to homogeneity as 3hydroxyhexobarbital dehydrogenase (Kageura & Toki, 1975).…”

“…In a previous paper we demonstrated that testosterone 17fi-dehydrogenase (NADP+) (EC 1.1.1.64), obtained from guinea-pig liver cytosol, participates in the reversible oxidation of 3-hydroxyhexobarbital [5-(3'-hydroxy-1'-cyclohexen-1'-yl)-1,5-dimethylbarbituric acid], the microsomal oxygenation product ofhexobarbital (Kageura & Toki, 1971). This enzyme was subsequently purified to homogeneity as 3hydroxyhexobarbital dehydrogenase (Kageura & Toki, 1975). During investigation of 3-hydroxyhexobarbital dehydrogenase as a testosterone 171)dehydrogenase (NADP+) (EC 1.1.1.64), another testosterone 17.8-dehydrogenase was isolated from the same source (Kageura & Toki, 1971, 1974).…”

“…This enzyme was subsequently purified to homogeneity as 3hydroxyhexobarbital dehydrogenase (Kageura & Toki, 1975). During investigation of 3-hydroxyhexobarbital dehydrogenase as a testosterone 171)dehydrogenase (NADP+) (EC 1.1.1.64), another testosterone 17.8-dehydrogenase was isolated from the same source (Kageura & Toki, 1971, 1974).…”