New aspects of galectin functionality in nuclei of cultured bone marrow stromal and epidermal cells: biotinylated galectins as tool to detect specific binding sites

Purkrábková, Tereza; Smetana, Karel; Dvořánková, Barbora; Holíková, Zuzana; Bock, Carolin; Lensch, M. William; André, Sabine; Pytlík, Robert; Liu, Fu‐Tong; Klíma, Jiřá; Motlı́k, Jan; Gabius, Hans‐Joachim

doi:10.1016/j.biolcel.2003.08.002

Cited by 73 publications

(52 citation statements)

References 57 publications

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“…Following routine inhibition of endogenous peroxidase activity and saturation of nonspecific protein-binding sites and biotin-binding sites, the tissue sections were incubated with the probes at room temperature for 60 min. Lectin-binding capacities of the tumor cells were studied with labeled (biotinylated) galectin-1 (Gal-1) and galectin-3 (Gal-3) prepared and checked for purity, extent of label incorporation and activity as described [15,16,17,18]. Hyaluronic-acid-binding capacity was investigated with biotinylated hyaluronic acid dissolved in medium which was either Ca 2+ free (HA) or contained 8 mmol/l Ca 2+ (HA+ Ca 2+ ) to test Ca 2+ -independent and Ca 2+ -dependent binding activities [19].…”

Section: Methodsmentioning

confidence: 99%

Prognostic Significance of Endogenous Adhesion/Growth-Regulatory Lectins in Lung Cancer

et al. 2005

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Objective: To determine the expression of endogenous adhesion/growth-regulatory lectins and their binding sites using labeled tissue lectins as well as the binding profile of hyaluronic acid as an approach to define new prognostic markers. Methods: Sections of paraffin-embedded histological material of 481 lungs from lung tumor patients following radical lung excision processed by a routine immunohistochemical method (avidin-biotin labeling, DAB chromogen). Specific antibodies against galectins-1 and -3 and the heparin-binding lectin were tested. Staining by labeled galectins and hyaluronic acid was similarly visualized by a routine protocol. After semiquantitative assessment of staining, the results were compared with the pT and pN stages and the histological type. Survival was calculated by univariate and multivariate methods. Results: Binding of galectin-1 and its expression tended to increase, whereas the parameters for galectin-3 decreased in advanced pT and pN stages at a statistically significant level. The number of positive cases was considerably smaller among the cases with small cell lung cancer than in the group with non-small-cell lung cancer, among which adenocarcinomas figured prominently with the exception of galectin-1 expression. Kaplan-Meier computations revealed that the survival rate of patients with galectin-3-binding or galectin-1-expressing tumors was significantly poorer than that of the negative cases. In the multivariate calculations of survival lymph node metastases (p < 0.0001), histological type (p = 0.003), galectin-3-binding capacity (p = 0.01), galectin-3 expression (p = 0.03) and pT status (p = 0.003) proved to be independent prognostic factors, not correlated with the pN stage. Conclusion: The expression and the capacity to bind the adhesion/growth regulatory galectin-3 is defined as an unfavorable prognostic factor not correlated with the pTN stage.

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Section: Methodsmentioning

confidence: 99%

Prognostic Significance of Endogenous Adhesion/Growth-Regulatory Lectins in Lung Cancer

et al. 2005

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Section: Glycobiology Of Normal Squamous Epitheliamentioning

confidence: 93%

“…Keratinocytes prepared from hair follicle sheath exhibited distinct intranuclear binding of Gal-1 colocalizing partially with SC35 splicing factor [23] under in vitro conditions. No similar phenomenon was observed in cultured cells prepared from the interfollicular epidermis [23]. These observations suggest a possibility of differentiation-dependent control of nuclear Gal-1-reactive epitope expression which can participate in pre-mRNA splicing in epithelial cells at the low differentiation grade.…”

Section: Glycobiology Of Normal Squamous Epitheliamentioning

confidence: 93%

Glycobiology of Head and Neck Squamous Epithelia and Carcinomas

Plzák

Smetana

Chovanec

et al. 2005

ORL

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An impressive variety of regulatory processes including cell adhesion and migration, proliferation, apoptosis and differentiation folding and routing of glycoproteins have been found to be mediated by specific lectin-carbohydrate interactions. This article summarizes the data on glycobiological aspects of differentiation of squamous epithelia in the head and neck region under physiological conditions and in cancer. The possible function of lectins in tumor development and invasiveness is debated. Introduction of labeled endogenous lectins as a tool for the study of functional glycomics at the cellular level in head and neck squamous epithelia and carcinomas enables a complex interpretation of studied data because these lectins are normally occurring in these tissues. The lectinology of Langerhans cells in head and neck squamous epithelia and carcinoma is also mentioned. Finally, the use of the described data in the diagnosis and prospectively in the treatment of head and neck squamous cell carcinoma is shown.

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“…Skin samples from the breast of 2 female donors were employed. NHF and NIF keratinocytes were prepared and subcultured as described previously (30).…”

Section: Methodsmentioning

confidence: 99%

Human hair follicle and interfollicular keratinocyte reactivity to mouse HPV16-transformed cells: An in vitro study

Smetana¹,

Dvořánková²,

Lacina³

et al. 2008

Oncol Rep

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Abstract. The role of stem cells in cancer formation and spreading has been established. As with normal tissue, the cancer stem cells need a special microenvironment to support their growth. This microenvironment may be represented by the tumor stroma. One of the possible ways of tumor stromal formation is the epithelial-mesenchymal transition of tumor epithelium. Following this mechanism, stromal cells must share the basic genetic alterations with the tumor cells. In an attempt to create a system capable of testing some aspects of the mesenchymal cell-keratinocyte interactions, we studied the effects of the fibroblastoid mouse TC-1 cells that were prepared by the introduction of human papillomavirus type 16 (HPV16) genes E6 and E7 to lung epithelial cells on the phenotype of normal human interfollicular and hair follicle keratinocytes. From this point of view, they may resemble stromal cells formed by the epithelial-mesenchymal transition of cells from HPV-induced squamous cell carcinoma. In contrast to 3T3 murine embryonic fibroblasts which were used as control cells, TC-1 cells influenced not only the size of the keratinocytes and the shape of their colonies, but also induced the expression of keratins 8 and 19 and vimentin. In conclusion, TC-1 cells exhibited a marked biological activity by influencing the behavior of the normal human follicular and intefollicular keratinocytes. This observation is compatible with the hypothesis that stromal cells play an important role in tumor progression and spreading.

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New aspects of galectin functionality in nuclei of cultured bone marrow stromal and epidermal cells: biotinylated galectins as tool to detect specific binding sites

Cited by 73 publications

References 57 publications

Prognostic Significance of Endogenous Adhesion/Growth-Regulatory Lectins in Lung Cancer

Prognostic Significance of Endogenous Adhesion/Growth-Regulatory Lectins in Lung Cancer

Glycobiology of Head and Neck Squamous Epithelia and Carcinomas

Human hair follicle and interfollicular keratinocyte reactivity to mouse HPV16-transformed cells: An in vitro study

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