New Aspects of HECT-E3 Ligases in Cell Senescence and Cell Death of Plants

Lan, Wei; Miao, Ying

doi:10.3390/plants8110483

Cited by 18 publications

(17 citation statements)

References 69 publications

(108 reference statements)

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“…Arabidopsis HECT-type ubiquitin ligase 5 (UPL5) is a member of the plant HECT-type ubiquitinated E3 ligase family, which is highly expressed in senescent leaves [ 9 ]. In comparing the amino acid sequences of animal and plant HECT-type ubiquitin ligases, it is found that there is no member corresponding to UPL5 of Arabidopsis HECT-type ubiquitinated E3 ligase family in yeast and animal genomes [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Landscape of UPL5-Ubiquitinated on Multiple Subcellular Components of Leaf Senescence Cell in Arabidopsis

Lan

Zheng

Yang

et al. 2022

IJMS

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Catabolism of macromolecules is a major event in senescent cells, especially involving proteolysis of organelles and abnormally aggregated proteins, circulation of nutrients, and precise control of intracellular environmental balance. Proteasomes are distributed in the nucleus and cytoplasm; however, proteasomes in organelles are limited. In this study, multi-omics proteomic analyses of ubiquitinated proteins enriched by using antibody against “di-Gly-Lys” via a free labeling were used to investigate the global changes of protein levels and ubiquitination modification levels of upl5 mutant relative to wild-type plant; subcellular localization analysis of UPL5 was found to be located in the nucleus, cytoplasm, and plastid within the cell; and the direct lysine site patterns of UPL5 were screened by the H89R substitution in the tagged ubiquitinated assay. It suggests that UPL5 acting as a candidate of organelle E3 ligase either in the nucleus or cytoplasm or plastid modifies numerous targets related to nuclear transcription and plastid photosynthesis involving in Ca2+ and hormone signaling pathway in plant senescence and in response to (a)biotic stress protection.

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Section: Introductionmentioning

confidence: 99%

Establishment of a Landscape of UPL5-Ubiquitinated on Multiple Subcellular Components of Leaf Senescence Cell in Arabidopsis

Lan

Zheng

Yang

et al. 2022

IJMS

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“…The ubiquitin-protein ligase enzyme (E3) directly removes the damaged proteins through the degradation pathway and participates in the protein quality-control pathway. At the same time, it also removes damaged proteins, chloroplasts, and other organelles in batches by regulating the key factors of autophagy and cell death [ 33 , 34 , 35 ], and participates in cell death regulation. The DEGs of the three comparison groups were annotated to the E3 ligase enzyme.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis of Air Space-Type Variegation Formation in Trifolium pratense

Zhang

Zou

et al. 2022

IJMS

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Air space-type variegation is the most diverse among the species of known variegated leaf plants and is caused by conspicuous intercellular spaces between the epidermal and palisade cells and among the palisade cells at non-green areas. Trifolium pratense, a species in Fabaceae with V-shaped air space-type variegation, was selected to explore the application potential of variegated leaf plants and accumulate basic data on the molecular regulatory mechanism and evolutionary history of leaf variegation. We performed comparative transcriptome analysis on young and adult leaflets of variegated and green plants and identified 43 candidate genes related to air space-type variegation formation. Most of the genes were related to cell-wall structure modification (CESA, CSL, EXP, FLA, PG, PGIP, PLL, PME, RGP, SKS, and XTH family genes), followed by photosynthesis (LHCB subfamily, RBCS, GOX, and AGT family genes), redox (2OG and GSH family genes), and nitrogen metabolism (NodGS family genes). Other genes were related to photooxidation, protein interaction, and protease degradation systems. The downregulated expression of light-responsive LHCB subfamily genes and the upregulated expression of the genes involved in cell-wall structure modification were important conditions for air space-type variegation formation in T. pratense. The upregulated expression of the ubiquitin-protein ligase enzyme (E3)-related genes in the protease degradation systems were conducive to air space-type variegation formation. Because these family genes are necessary for plant growth and development, the mechanism of the leaf variegation formation in T. pratense might be a widely existing regulation in air space-type variegation in nature.

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“…Ubiquitin and ubiquitin-like modification is a dynamic and reversible process involving the covalent attachment of a conserved 76-amino acid peptide ubiquitin (ub) to the ε-amino groups of lysine residues in target proteins. Ubiquitin addition is catalyzed by an enzymatic cascade involving an E1 ub-activating enzyme, followed by the sequential activities of E2 ub-conjugating enzyme and E3 ub ligase (Smalle and Vierstra, 2004 ; Lan and Miao, 2019 ). This process is reversible as a result of deubiquitinating enzymes (DUBs).…”

Section: The Regulation Of As Through Ptmsmentioning

confidence: 99%

Ubiquitination and Ubiquitin-Like Modifications as Mediators of Alternative Pre-mRNA Splicing in Arabidopsis thaliana

Lan

Qiu

et al. 2022

Front. Plant Sci.

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Alternative splicing (AS) is a common post-transcriptional regulatory process in eukaryotes. AS has an irreplaceable role during plant development and in response to environmental stress as it evokes differential expression of downstream genes or splicing factors (e.g., serine/arginine-rich proteins). Numerous studies have reported that loss of AS capacity leads to defects in plant growth and development, and induction of stress-sensitive phenotypes. A role for post-translational modification (PTM) of AS components has emerged in recent years. These modifications are capable of regulating the activity, stability, localization, interaction, and folding of spliceosomal proteins in human cells and yeast, indicating that PTMs represent another layer of AS regulation. In this review, we summarize the recent reports concerning ubiquitin and ubiquitin-like modification of spliceosome components and analyze the relationship between spliceosome and the ubiquitin/26S proteasome pathway in plants. Based on the totality of the evidence presented, we further speculate on the roles of protein ubiquitination mediated AS in plant development and environmental response.

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New Aspects of HECT-E3 Ligases in Cell Senescence and Cell Death of Plants

Cited by 18 publications

References 69 publications

Establishment of a Landscape of UPL5-Ubiquitinated on Multiple Subcellular Components of Leaf Senescence Cell in Arabidopsis

Establishment of a Landscape of UPL5-Ubiquitinated on Multiple Subcellular Components of Leaf Senescence Cell in Arabidopsis

Transcriptome Analysis of Air Space-Type Variegation Formation in Trifolium pratense

Ubiquitination and Ubiquitin-Like Modifications as Mediators of Alternative Pre-mRNA Splicing in Arabidopsis thaliana

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