New Attachment Achieved by Guided Tissue Regeneration in Beagle Dogs

Abstract

This study tested the effects of using a Gore-Tex periodontal material on new connective tissue attachment in beagle dogs with natural periodontitis. Seven female beagle dogs with advanced periodontitis were selected and given thorough root debridement. Under general anesthesia four weeks later, mucoperiosteal flaps were raised involving all mandibular premolars and first molars. Gore-Tex periodontal material was adapted to all premolars, and the flaps were sutured tightly, keeping the material covered. The first molars acted as controls, receiving only surgery. Some material was removed in one month, while other materials were removed eight to 10 weeks after surgery. All dogs were sacrificed at three months. After processing, descriptive histology and histometrics were carried out, evaluating new connective tissue attachment, bone response and epithelial downgrowth. Results were statistically analyzed, using paired t tests. They showed new connective tissue attachment with cementum deposition in areas where the material was used. Also, the epithelial downgrowth was reduced in these areas. In the controls, minimal connective tissue attachment was found, with the area healing by a long junctional epithelium. Statistical analysis showed significant differences (P less than 0.05) favoring experimental areas both in the increase of new connective tissue attachment and in the decrease of epithelial downgrowth. No differences were found between areas where the material was removed in one month or left longer in place. No difference in bone response was found. Gore-Tex material was effective in blocking gingival epithelial downgrowth and connective tissue proliferation, promoting new attachment according to the principle of "guided tissue regeneration."

Furcation Defects in Dogs Treated by Guided Tissue Regeneration (GTR)

“…the repopulation of the wound area adjacent to the roots by cells originating from the periodontal ligament. Similar results were obtained by Aukhil et al14 and Caffesse et al 15 following the use of the GTR procedure in beagle dogs.…”

Effect of Early Membrane Removal on the Treatment of Mandibular Class II Furcation Defects - A Controlled Clinical Trial with Re-entry after 12 Months

“…This study indicated that both resorbable and non-resorbable membranes were more effective than open flap debridement for reducing vertical probing depths, gaining vertical attachment levels and gaining vertical and horizontal bone in class II furcation regeneration (18). ePTFE membranes have also demonstrated an adequate clinical performance, being able of inhibiting the down growth of epithelium, promoting the isolation of the gingival connective tissue, and protection of the blood clot (8,19). Replacement of the blood clot eventually leads to the formation of new periodontal tissues.…”

“…The period of membrane removal varies from 4 to 6 weeks after placement (6) and longer time periods do not provide additional benefits (8). The exposure of the membrane in the early healing phase has been negatively correlated with the amount of newly formed tissues under the barrier (9), which occurs due to bacterial contamination of the membrane.…”

Effect of autogenous tooth bone graft with membrane on Class II furcation defects in dogs: A histologic and histomorphometric study

“…Moreover, previous reports suggest that membrane maintenance for 6 weeks or longer has no additional benefit [40]. In the present study, the membrane was retained until 8 weeks , when the experiment was completed.…”