New autoantigens in rheumatoid arthritis (RA): screening 8268 protein arrays with sera from patients with RA

Abstract

We identified PAD4 and BRAF as RA specific autoantigens.

Novel autoantibody markers for early and seronegative rheumatoid arthritis

“…The recent finding by Auger et al of autoantibodies against another tumor-antigen BRAF in RA patients, further underscores the importance of our findings [41]. Interestingly, one-third of the anti-BRAF antibodypositive RA patients were ACCP-negative [41].…”

“…In view of this increased oncogene expression in RA synovium and the finding of a thicker synovial lining in ACCPnegative RA, antibodies against candidate oncogenes can be seen as a consequence of loss of tolerance or as part of a protective immunity against hyperproliferating cells [40]. The recent finding by Auger et al of autoantibodies against another tumor-antigen BRAF in RA patients, further underscores the importance of our findings [41]. Interestingly, one-third of the anti-BRAF antibodypositive RA patients were ACCP-negative [41].…”

Identification of a novel autoantigen in inflammatory bowel disease by protein microarray

“…[15][16][17][18][19] In an attempt to identify novel autoantibody biomarkers in IBD patients, we evaluated the autoimmune profile in a limited cohort of patients (10 UC and 15 CD) and controls (n ¼ 5) by means of a protein microarray containing 8268 human proteins. In all, 256 antigens were identified to which IBD patients had a higher seroreactivity than controls.…”

Role of citrullination modification catalyzed by peptidylarginine deiminase 4 in gene transcriptional regulation

“…The role of PADI4 in early RA pathogenesis is mainly through promoting the generation of citrullinated autoantigens [57,58]. PADI4 has been identified as a new therapeutic target for RA treatment [59][60][61][62]. PADI4 is also overexpressed in the multiple sclerosis [63,64], and heterozygote genotypes for PADI4_89 were protectively associated with susceptibility to tuberculosis in Koreans [65]; however, the underlying mechanism remains unclear.…”