New bicyclic [3.2.1] octane neolignans derivatives from Aniba firmula with potent in vivo anti-inflammatory activity on account of dual inhibition of PGE2 production and cell recruitment

Santos, Mário F. C.; Alcântara, Bianca Gonçalves Vasconcelos de; Feliciano, Cristiane dos R.; Silva, Aline F.; Maiolini, Tatiane C. S.; Neto, Albert K.; Murgu, Michael; Paula, Daniela Aparecida Chagas de; Soares, Marisi G.

doi:10.1016/j.phytol.2019.01.014

Cited by 16 publications

(11 citation statements)

References 16 publications

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“…This assay was carried out with male Swiss mice, chosen according to a previously described methodology 23,24 . The Ethics Committee for the Use of Animals at the Federal University of Alfenas (Unifal‐MG) approved the protocol (number 16/2016).…”

Section: Methodsmentioning

confidence: 99%

“…Initially, cutaneous inflammation was induced in the left ear of the mice ( n = 8) with the topical application of croton oil (5% v/v ) dissolved in acetone (vehicle), according to the established protocols by Tubaro et al . and Santos et al 23,24 . Only acetone was applied in the right ear.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, 30 min after the application of 20 μL of the irritant solution (croton oil), topical treatment took place with the plant extracts or reference drugs (positive controls). Reference drugs (dexamethasone and indomethacin), as well as tested extracts, were all diluted in acetone (vehicle) at 0.5 mg/ear 23,24 . Animals from the negative control group received only the vehicle acetone as treatment.…”

Section: Methodsmentioning

confidence: 99%

“…This assay was carried out with male Swiss mice, chosen according to a previously described methodology. 23,24 The Ethics Committee for 25 Although croton oil and TPA are effective as phlogistic agents for antiinflammatory assay, croton oil is cheaper than TPA. Our study intends to develop an approach that is the cheapest and simplest for the assessment of the anti-inflammatory activity.…”

Section: In Vivo Anti-inflammatory Assay For the Ear Edema Inhibition Assessmentmentioning

confidence: 99%

“…Reference drugs (dexamethasone and indomethacin), as well as tested extracts, were all diluted in acetone (vehicle) at 0.5 mg/ear. 23,24 Animals from the negative control group received only the vehicle acetone as treatment.…”

Section: In Vivo Anti-inflammatory Assay For the Ear Edema Inhibition Assessmentmentioning

confidence: 99%

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In vivo anti‐inflammatory activity of Fabaceae species extracts screened by a new ex vivo assay using human whole blood

Rosa

Domingos

Salem

et al. 2021

Phytochemical Analysis

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Introduction Plants have been considered a promising source for discovering new compounds with pharmacological activities. The Fabaceae family comprises a large variety of species that produce substances with diverse therapeutic potential, including anti‐inflammatory activity. The limitations of current anti‐inflammatories generate the need to research new anti‐inflammatory structures with higher efficacy as well as develop methods for screening multiple samples, reliably and ethically, to assess such therapeutic properties. Objective Validate and apply a quantification method for prostaglandin E2 (PGE2) production from an ex vivo assay in human blood in order to screen anti‐inflammatory activity present in many Fabaceae species extracts. Methods Human blood was incubated with extracts from 47 Fabaceae species. After lipopolysaccharide (LPS)‐induced inflammation, PGE2 was quantified in the plasma by liquid chromatography with tandem mass spectrometry (LC–MS/MS). The extracts that presented PGE2 production inhibition were further assessed through in vivo assay and then chemically characterised through an analysis of ultra‐performance liquid chromatography electrospray ionisation quadrupole time‐of‐flight tandem mass spectrometry (UPLC‐ESI‐QTOF‐MS2) data. Results The new ex vivo anti‐inflammatory assay showed that five out of the 47 Fabaceae species inhibited PGE2 production. Results from an in vivo assay and the metabolic profile of the active extracts supported the anti‐inflammatory potential of four species. Conclusion The quantification method for PGE2 demonstrated fast, sensitive, precise, and accurate results. The new ex vivo anti‐inflammatory assay comprised a great, reliable, and ethical approach for the screening of a large number of samples before an in vivo bioassay. Additionally, the four active extracts in both ex vivo and in vivo assays may be useful for the development of more efficient anti‐inflammatory drugs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: In Vivo Anti-inflammatory Assay For the Ear Edema Inhibition Assessmentmentioning

confidence: 99%

Section: In Vivo Anti-inflammatory Assay For the Ear Edema Inhibition Assessmentmentioning

confidence: 99%

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In vivo anti‐inflammatory activity of Fabaceae species extracts screened by a new ex vivo assay using human whole blood

Rosa

Domingos

Salem

et al. 2021

Phytochemical Analysis

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Anti‐Inflammatory Activity of Lauraceae Plant Species and Prediction Models Based on Their Metabolomics Profiling Data

Alcântara

Katchborian-Neto

Garcia

et al. 2023

Chemistry & Biodiversity

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The Lauraceae is a botanical family known for its anti‐inflammatory potential. However, several species have not yet been studied. Thus, this work aimed to screen the anti‐inflammatory activity of this plant family and to build statistical prediction models. The methodology was based on the statistical analysis of high‐resolution liquid chromatography coupled with mass spectrometry data and the ex vivo anti‐inflammatory activity of plant extracts. The ex vivo results demonstrated significant anti‐inflammatory activity for several of these plants for the first time. The sample data were applied to build anti‐inflammatory activity prediction models, including the partial least square acquired, artificial neural network, and stochastic gradient descent, which showed adequate fitting and predictive performance. Key anti‐inflammatory markers, such as aporphine and benzylisoquinoline alkaloids were annotated with confidence level 2. Additionally, the validated prediction models proved to be useful for predicting active extracts using metabolomics data and studying their most bioactive metabolites.

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In vivo schistosomicidal activity of (±)-licarin A-loaded poly(ε-caprolactone) nanoparticles

Lima

Magalhães

Paula

et al. 2022

Experimental Parasitology

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New bicyclic [3.2.1] octane neolignans derivatives from Aniba firmula with potent in vivo anti-inflammatory activity on account of dual inhibition of PGE2 production and cell recruitment

Cited by 16 publications

References 16 publications

In vivo anti‐inflammatory activity of Fabaceae species extracts screened by a new ex vivo assay using human whole blood

In vivo anti‐inflammatory activity of Fabaceae species extracts screened by a new ex vivo assay using human whole blood

Anti‐Inflammatory Activity of Lauraceae Plant Species and Prediction Models Based on Their Metabolomics Profiling Data

In vivo schistosomicidal activity of (±)-licarin A-loaded poly(ε-caprolactone) nanoparticles

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