New biodegradable small‐diameter artificial vascular prosthesis: A feasibility study

Abstract

An in-house built instrument was used to fabricate a small internal diameter (2 mm) artificial vascular prosthesis from biodegradable chitosan. This new artificial vascular prosthesis has shown a good biocompatibility based on the studies of its cell compatibility, inflammatory reaction, and platelet adhesion. In an animal test, the prosthesis was used to replace a 4-cm-long section of femoral artery in each of the seven tested dogs. The patency of the replacement was monitored at regular intervals using Doppler ultrasound diagnostics. Nine months after the implantation, hematoxylin and eosin staining, immunohistochemical study, and scanning electron microscope observation were carried out. Complete decomposition of the prosthesis and replacement by a natural blood vessel were observed. The results suggests that the artificial vascular prosthesis displays many characteristics of the "ideal" small-diameter artificial vascular, and have the biocompatibility that can be tailored to match those desired in vascular replacement application.

Suppression of Magnaporthe oryzae and interaction between Bacillus subtilis and rice plants in the control of rice blast

“…For scanning electron microscopy (SEM) examination, the co-culture samples that blended B. subtilis SYX04 and SYX20 cells (1 × 10 8 CFU/mL) and spore suspension of M. oryzae P131 (1 × 10 6 CFU/mL) by the same volume were incubated for 12 h in culture conditions as described previously, and M. oryzae P131 without B. subtilis cells served as a control. The conidial and hyphal structures of M. oryzae P131 inhibited by B. subtilis SYX04 and SYX20 cells were fixed in 4 % glutaraldehyde at 4 °C for 16 h, dehydrated, and coated with gold as described previously (Kong et al 2012 ). The samples were observed with an S-3400N (Hitachi, Tokyo, Japan) scanning electron microscope as previously described (Kong et al 2012 ).…”

“…The conidial and hyphal structures of M. oryzae P131 inhibited by B. subtilis SYX04 and SYX20 cells were fixed in 4 % glutaraldehyde at 4 °C for 16 h, dehydrated, and coated with gold as described previously (Kong et al 2012 ). The samples were observed with an S-3400N (Hitachi, Tokyo, Japan) scanning electron microscope as previously described (Kong et al 2012 ). For transmission electron microscopic (TEM) examination, the co-culture samples that contained both B. subtilis SYX04 or SYX20 cells (1 × 10 8 CFU/mL) and spore suspension of M. oryzae P131 (1 × 10 6 CFU/mL) at the same volume were incubated for 4, 8, 12, and 24 h in culture conditions as described previously, and the co-cultures without B. subtilis cells served as a controls.…”

Fabrication and feasibility study of an absorbable diacetyl chitin surgical suture for wound healing

“…The animal experiments in this study were carried out in compliance with the National Institute of Health Guide for the Care and Use of Laboratory Animals. Thirty‐six adult male Sprague‐Dawley rats were housed singly in standard cages and kept under controlled temperature and humidity with free access to food and water . The rats were anesthetized with intraperitoneal administration of pentobarbital sodium (3% in saline solution) at a dose of 1 mL/kg.…”

“…Thirty-six adult male Sprague-Dawley rats were housed singly in standard cages and kept under controlled temperature and humidity with free access to food and water. 25 The rats were anesthetized with intraperitoneal administration of pentobarbital sodium (3% in saline solution) at a dose of 1 mL/kg. The dorsal side of the rats was shaved and disinfected with 75% alcohol for surgery.…”

Mechanical and Biocompatibility Performance of Bicomponent Polyester/Silk Fibroin Small-Diameter Arterial Prostheses

“…At present, an increasing number of people are affected by cardiovascular disease. It is reported that in 2003 about 1 million people died from cardiovascular disease, and nearly 84 million people were affected by this disease, with another 13.2 million people suffering from coronary artery disease (1). Transplantation is an effective way to treat this condition, but it requires autologous vessels to avoid immunological complications and rejection.…”