New biodiagnostics based on optical tweezers: typing red blood cells, and identification of drug resistant bacteria

Chen, Jia Wen; Lin, Chuen Fu; Wang, Shyang Guang; Lee, Yi Chieh; Chiang, Chung Han; Huang, Min; Lee, Yi Hsiung; Vitrant, G.; Pan, Ming; Lee, Horng-Mo; Liu, Yi Jui; Baldeck, Patrice L.; Lin, Chih Lang

doi:10.1117/12.2027951

Cited by 2 publications

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“…In this study, we extended our preliminary works [33][34][35] B3, the most common B subtype in the Asian population, was used as the subject of subtype identi cation [11].…”

Section: Introductionmentioning

confidence: 91%

“…In this study, we extended our preliminary works [33][34][35] based on the bonding of speci c antigenprotein/antibody interactions and optical manipulations to develop an alternative methodology to analyze antigen expression on the whole RBC. Antigen expression was quantitatively estimated using the optical detachment technique to systematically measure serial bond strengths between a single RBC and corresponding antibody-coated slides.…”

Section: Introductionmentioning

confidence: 99%

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A quantitative methodology to detect surface antigens on red blood cells using optical cell-detachment technique

Lin¹,

Wang²,

Tien³

et al. 2020

Preprint

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The quantitative analysis of surface antigens on cells, especially red blood cells (RBCs), has attracted increasing attention due to the recognition of antigenic changes that can facilitate early diagnoses. This paper presents an alternative methodology developed using the optical cell-detachment technique to evaluate antibody-antigen interactions and quantitatively analyze the RBC surface antigen expression. RBC subtyping was used to verify the proposed detection principle based on a comparison of the bonding strengths between individual RBCs and antibody coatings. The bonding strengths were measured with serial antibody dilutions with gradually decreasing laser powers, for which a single cell was optically detached from the corresponding antibody-coated surface. With the quantitatively analysis, the proposed alternative methodology was verified as a highly sensitive technique for detecting antigen expression on the RBC surface.

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“…In this study, we extended our preliminary works [33][34][35] B3, the most common B subtype in the Asian population, was used as the subject of subtype identi cation [11].…”

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

A quantitative methodology to detect surface antigens on red blood cells using optical cell-detachment technique

Lin¹,

Wang²,

Tien³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, we extended our preliminary sequent works [31][32][33] based on the speci c antigen-antibody interactions principle and optical manipulation technique to develop an alternative methodology for estimating the variation of antigen expressions on RBCs. Herein, the optical tweezers was employed as a biosensor to detach an individual RBC off from an antibody-coated surface with serial dilution folds and laser powers for detecting the RBC binding.…”

Section: Introductionmentioning

confidence: 99%

A simple and intuitive methodology for estimating red blood cell surface antigen expression variation using optical cell-detachment technique

Lin

Wang

TIEN³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

The analysis of surface antigens on cells, especially red blood cells (RBCs), has attracted increasing attention due to the recognition of antigenic variation that can facilitate early diagnoses. This paper presents an alternative methodology to estimate the variation of surface antigen expressions using an optical cell-detachment technique to validate the binding of individual RBCs stuck on corresponding antibody-coated surfaces. The detachment tests were implemented by an optical tweezers with gradually decreasing laser powers associated with serial antibody dilutions. Then, the antigen expression variation was estimated based on the known antibody dilution folds. The B- and B3-types of RBCs were selected for the demonstration subjects. With the semi-quantitative analysis, the proposed methodology was successfully verified for evaluating the variation of the RBC surface antigen expressions. The analysis result shows good consistency with the literature’s findings.

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New biodiagnostics based on optical tweezers: typing red blood cells, and identification of drug resistant bacteria

Cited by 2 publications

References 0 publications

A quantitative methodology to detect surface antigens on red blood cells using optical cell-detachment technique

A quantitative methodology to detect surface antigens on red blood cells using optical cell-detachment technique

A simple and intuitive methodology for estimating red blood cell surface antigen expression variation using optical cell-detachment technique

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