New biomaterial based on cotton with incorporated Biomolecules

Abstract

The aim of this study was to investigate a method of embedding l‐cysteine (l‐cys), an antimicrobial agent, between layers of chitosan (CH) and sodium alginate (ALG) onto cotton samples obtained via a layer‐by‐layer electrostatic deposition technique via several embedding methods. The results show that the best way to incorporate l‐cys into the layers was the one that used the property of gelling ALG. To monitor the l‐cys embedding into the CH/ALG multilayer film, different methods were used: energy‐dispersive X‐ray spectrometry analysis to assess the presence of sulfur on the sample, Ellman's reagent method to analyze l‐cys release from the sample, and attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR) to compare the ATR–FTIR spectra of the pure l‐cys and l‐cys embedded in the CH/ALG multilayer film to study the interaction between the l‐cys and the CH/ALG multilayer films. Functionalized CH/ALG cotton samples were also investigated for their antibacterial properties toward Staphylococcus aureus and Klebsiella pneumonia with the Japanese Industrial Standard method JIS L 1902:2002, and the results show an enhancement of the antibacterial effect due to the presence of l‐cys. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40519.

Antimicrobial Food Packaging Based on Prodigiosin-Incorporated Double-Layered Bacterial Cellulose and Chitosan Composites

“…The spectra obtained are shown in Figure 3, and BC spectra present a broad absorption peak centered at 3334 cm −1 , which corresponds to O-H stretching vibration, the peak at 2979 cm −1 was attributed to C-H stretching vibration, CH2 asymmetric stretching was identified at 2887 cm −1 , characteristic absorption bands appear at 1082 cm −1 , assigned to C-C bonds of polysaccharide monomer units, the peak at 1420 cm −1 was attributed to symmetric CH2 bending vibration, and the infrared band observed at 668 cm −1 was assigned to C-OH out of plane bending [59,62,63]. The characteristic band of carboxylate (COO -) was identified at 1620 cm −1 , indicating BC oxidation by TEMPO [23,64]. In PVA_CH spectra a broad, but low-intensity peak, is observed around 3011-3517 cm −1 , assigned to O-H and N-H stretching vibrations, the peak at 2881 cm −1 was attributed to C-H stretching vibration, the band observed at 1464 cm −1 corresponds to amide groups of CH, also the band at 1341 cm −1 is attributed to C-O bond of CH, the absorption band at 1240 cm −1 is assigned to O-H bending vibration, C-O stretching vibration was identified at 1094 cm −1 , and C-C stretching of PVA occurs at 841 cm −1 [65][66][67][68].…”

Agrimonia Eupatoria L.-Incorporated Electrospun Nanofibers and Cotton Composite for Antibacterial Wound Dressing Applications

“…materials. Firstly, the surface of cotton dressing was oxidized using the 0.0125% (w/v) TEMPO/0.125% (w/v) NaBr/ 3.2% (v/v) NaClO system at pH 10.5, one of the most common surface chemical modification methods of cotton fibers, which directly oxidizes the primary alcohol of cellulose to negatively-charged carboxyl groups, increasing the cotton negative surface charge density [5,6].…”

“…To overcome such limitations and improve their application as wound dressings, different surface modifications have been reported. Among the various techniques, the chemical modification of cotton using 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) has received considerable attention due to its ability to increase the negative charge density onto the cotton surface [5,6]. This enables the cotton fibers to be functionalized with other materials, namely with electrospun nanofibers which exhibit structural features similar to those presented by the native skin's extracellular matrix (ECM).…”

Incorporation of antimicrobial peptides on functionalized cotton gauzes for medical applications

“…Functionalization of cotton gauzes was prepared with four layers (cotton gauze/CH/ALG/CH/ALG), according with the optimization made in our previous work (Gomes, Mano, Queiroz, & Gouveia, 2010;Gomes et al, 2012;Gomes, Mano, Queiroz, & Gouveia, 2013;Gomes, Mano, Queiroz, & Gouveia, 2014). Then, the functionalized cotton gauze was immersed in a solution of AMPs in concentration higher than the MIC values, 10 g/mL for 24 h. The selected concentration aimed to ensure that a significant amount would be absorb, giving the expected antimicrobial activity.…”