2017
DOI: 10.3389/fpls.2017.01418
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New Biotechnological Tools for the Genetic Improvement of Major Woody Fruit Species

Abstract: The improvement of woody fruit species by traditional plant breeding techniques has several limitations mainly caused by their high degree of heterozygosity, the length of their juvenile phase and auto-incompatibility. The development of new biotechnological tools (NBTs), such as RNA interference (RNAi), trans-grafting, cisgenesis/intragenesis, and genome editing tools, like zinc-finger and CRISPR/Cas9, has introduced the possibility of more precise and faster genetic modifications of plants. This aspect is of… Show more

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Cited by 124 publications
(88 citation statements)
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References 185 publications
(196 reference statements)
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“…Such an approach opens up the possibility of producing non-transgenic fruit while ensuring the overall resistance to viral infection using a transgenic ribonucleic acid interference (RNAi) inducing rootstock. The success of this approach, however, has largely depended on the species, the level of expression and the interactions of the small interfering ribonucleic acid (siRNA) with the target gene [15]. For example, the transfer of transgene-derived siRNAs from the transgenic cherry rootstocks to the non-transgenic scions in grafted trees was effective in inducing resistance to the Prunus necrotic ringspot virus [16].…”
Section: Introductionmentioning
confidence: 99%
“…Such an approach opens up the possibility of producing non-transgenic fruit while ensuring the overall resistance to viral infection using a transgenic ribonucleic acid interference (RNAi) inducing rootstock. The success of this approach, however, has largely depended on the species, the level of expression and the interactions of the small interfering ribonucleic acid (siRNA) with the target gene [15]. For example, the transfer of transgene-derived siRNAs from the transgenic cherry rootstocks to the non-transgenic scions in grafted trees was effective in inducing resistance to the Prunus necrotic ringspot virus [16].…”
Section: Introductionmentioning
confidence: 99%
“…The development of new genome editing technologies in plant breeding has generated a growing interest in in vitro culture for regeneration protocols [13,14]. This is partially due to the difficulties often encountered in plant regeneration, which is a key step in any transformation protocol and a major bottleneck for applying these techniques in many species [13,15]. On the other hand, other breeding techniques, like the development of polyploids without antimitotic products such as colchicine [16][17][18][19], can benefit from regeneration protocols able to induce a certain percentage of plants with changes in ploidy levels.…”
Section: Introductionmentioning
confidence: 99%
“…Introduction of new traits in plants largely relied on sexual crosses between different genotypes within or between closely related species [1]. However, due to the presence of various reproductive barriers, gene transfer has been restricted to sexually-compatible species, thus limiting the possibilities of modifying and improving crop plants [4]. Many desirable and agronomicallyinteresting traits may only be found in distantly related species or even in unrelated plants [5].…”
Section: Introductionmentioning
confidence: 99%