New butyrylcholinesterase inhibitor derived from mulberry twigs, a kind of agricultural byproducts

Zhu, Yao; Xiang, Wei; Shen, Yao; Jia, Yongxing; Zhang, Yuansong; Zeng, Lingshu; Chen, Jiaxin; Zhou, You; Xue, Xiao; Huang, Xi; Xu, Li

doi:10.1016/j.indcrop.2022.115535

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…The monomers kuwanon G, kuwanon C, kuwanon H, and morusin were isolated and identified by us from mulberry twigs of Jialing 20 . The α-glucosidase inhibitory activity of monomers was determined according to the method described in the previous study with slight modifications .…”

Section: Methodsmentioning

confidence: 99%

“…The monomers kuwanon G, kuwanon C, kuwanon H, and morusin were isolated and identified by us from mulberry twigs of Jialing 20. 23 The α-glucosidase inhibitory activity of monomers was determined according to the method described in the previous study with slight modifications. 15 The samples to be tested, α-glucosidase (0.5 U•mL −1 ) and PBS (67 mM, PH 6.8), were fully mixed with 50 μL each and kept at 37 °C for 15 min, and then 50 μL of p-NPG (6 mM) was added and reacted at 37 °C for 15 min.…”

Section: Analysis Of Uplc-ms/msmentioning

confidence: 99%

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Rapid Screening of Nonalkaloid α-Glucosidase Inhibitors from a Mulberry Twig Extract Using Enzyme-Functionalized Magnetic Nanoparticles Coupled with UPLC-MS/MS

Feng

Xiang

Han

et al. 2022

J. Agric. Food Chem.

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Mulberry twigs are an important source of α-glucosidase inhibitors. To date, research studies on α-glucosidase in mulberry twigs have mainly focused on alkaloids such as 1-deoxynojirimycin (DNJ). Preliminary studies have shown that there may be more active nonalkaloid α-glucosidase inhibitors in mulberry twigs. In this study, we immobilized α-glucosidase on Fe3O4@SiO2 for the first time and rapidly screened four nonalkaloid α-glucosidase inhibitors (kuwanon G, kuwanon C, kuwanon H, and morusin) using ligand fishing technology with ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) from the mulberry twig extract of Jialing 20, the excellent artificial triploid variety of mulberry cultivated extensively in Southwest China. The half maximal inhibitory concentrations (IC50) of kuwanon H and kuwanon G were 2.82 ± 0.68 and 2.83 ± 0.31 μM, respectively, with better inhibition activity than that of DNJ (with an IC50 of 7.04 ± 0.82 μM). Meanwhile, the molecular docking results showed that the action sites of these two isopentenyl flavonoids on α-glucosidase were different from that of DNJ. In brief, this work is beneficial to discovering new α-glucosidase inhibitors from mulberry twigs quickly and accurately and provides a theoretical basis for the mulberry twig extract as a functional food or a natural hypoglycemic drug source, as well as a reference for directional breeding of mulberry, which greatly improves the exploitation and utilization value of mulberry twigs as an agricultural byproduct in the fields of agricultural production, functional food, and natural medicine.

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Section: Methodsmentioning

confidence: 99%

Section: Analysis Of Uplc-ms/msmentioning

confidence: 99%

Rapid Screening of Nonalkaloid α-Glucosidase Inhibitors from a Mulberry Twig Extract Using Enzyme-Functionalized Magnetic Nanoparticles Coupled with UPLC-MS/MS

Feng

Xiang

Han

et al. 2022

J. Agric. Food Chem.

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“…Jialing 20 is an arti cial triploid mulberry variety widely planted in Southwest China. In previous studies, we analyzed the small molecular components of its branch extract [5] and identi ed its active components that inhibit α-glucosidase[6] and butyrylcholinesterase [7]. However, there are few reports on the structure and activity of its polysaccharide.…”

Section: Introductionmentioning

confidence: 99%

Purification, structural characterization, and immunoregulatory activity of a polysaccharide from mulberry branch

Xiang,

Xu,

Zheng

et al. 2024

Preprint

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In view of the fact that mulberry branch has not been effectively utilized and polysaccharide is one of the main active components in mulberry branch, this study aims to reveal the structure and immunomodulatory activity of its polysaccharide. A type of neutral polysaccharides, named Mulberry Branch Polysaccharide-2 (MBP-2), was separated from mulberry branch using DEAE-52 and Sephadex G-100. As analyzed, they were mainly composed of glucose with a molecular weight of approximately 21.7 kDa. Methylation analysis demonstrated that MBP-2 primary contained a →4)-α-D-Glcp-(1→, α-D-Glcp-(1→ and →4, 6)-α-D-Glcp-(1→ structure, which was validated by nuclear magnetic resonance (NMR). In addition, cellular experiments indicated that MBP-2 significantly enhanced the production of NO, TNF-α and IL-6 in RAW264.7 cells, unraveling the potential immunoregulatory activity of MBP-2. Further analysis showed that MBP-2 exerted their immunoregulatory activity mainly via binding with TLR4 to activate the downstream TRIF-dependent signaling pathways.

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Rapid screening and sensing of stearoyl-CoA desaturase 1 (SCD1) inhibitors from ginger and their efficacy in ameliorating non-alcoholic fatty liver disease

Zeng,

Wang,

Peng

et al. 2024

Food Measure

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Non-alcoholic fatty liver disease is a prevalent chronic metabolic condition, for which no approved medications are available. As a condiment and traditional Chinese medicine, ginger can be useful in reducing the symptoms of non-alcoholic fatty liver disease. Although its active ingredients and mechanisms of action are unknown, there is a lack of research on them. The purpose of this study is to prepare magnetite (Fe3O4)@Stearoyl-CoA desaturase 1 (SCD1) materials and analyze them using ultra-high performance liquid-chromatography-mass spectrometry (UPLC-MS) for rapid screening of potential inhibitors of SCD1 in ginger. Based on this analysis, it has been shown that the primary components in ginger that bind SCD1 directly are gingerols, with 10-gingerol having a greater affinity for binding to SCD1 than 8-gingerol and 6-gingerol. Moreover, further studies indicated that free fatty acids (FFA)-induced lipid accumulation is improved by this class of compounds in normal human hepatocytes (THLE-3), with 10-gingerol being the most effective compound. This study provides a new insight into the mechanism, by which ginger contributes to the improvement of non-alcoholic fatty liver disease (NAFLD) and provide support for the effective use of 10-gingerol for the treatment of NAFLD.

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New butyrylcholinesterase inhibitor derived from mulberry twigs, a kind of agricultural byproducts

Cited by 9 publications

References 36 publications

Rapid Screening of Nonalkaloid α-Glucosidase Inhibitors from a Mulberry Twig Extract Using Enzyme-Functionalized Magnetic Nanoparticles Coupled with UPLC-MS/MS

Rapid Screening of Nonalkaloid α-Glucosidase Inhibitors from a Mulberry Twig Extract Using Enzyme-Functionalized Magnetic Nanoparticles Coupled with UPLC-MS/MS

Purification, structural characterization, and immunoregulatory activity of a polysaccharide from mulberry branch

Rapid screening and sensing of stearoyl-CoA desaturase 1 (SCD1) inhibitors from ginger and their efficacy in ameliorating non-alcoholic fatty liver disease

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