Mermithid parasitoids are well known to infect spiders, however, their impact on the hosts and their taxonomic identity are still poorly analyzed. We present the first record of a mermithid nematode infection in the spider genera Piratula (Lycosidae) and Coelotes (Agelenidae), and in the species Alopecosa pulverulenta and Pardosa paludicola (Lycosidae). We describe the interesting maldevelopment of the spiders’ female genitalia induced by the parasitoid and summarize the data on the impact of nematode parasitoids on spider development and behaviour. Phylogenetic analysis, based on the 18S rRNA, showed that spider’ parasitoids arose independently in different phylogenetic branches of the family Mermithidae.