New cell lines derived from the black cutworm, Agrotis ipsilon, that support replication of the A. ipsilon multiple nucleopolyhedrovirus and several group I nucleopolyhedroviruses

Harrison, Robert L.; Lynn, Dwight E.

doi:10.1016/j.jip.2008.02.015

Cited by 17 publications

(10 citation statements)

References 48 publications

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“…Only the N-terminal half of the upstream egt ORF exhibited sequence similarity with other NPV EGT sequences. To confirm this arrangement in AgipMNPV, the corresponding region of the egt gene was amplified by PCR from OV DNA and from the BV DNA of 20 AgipMNPV plaque isolates generated during a previous study [17]. PCR and sequencing of amplimers revealed two genotypes in the OV DNA from AgipMNPV polyhedra (Fig.…”

Section: Genetic Variation In the Agipmnpv Illinois Isolatementioning

confidence: 88%

“…DNAs from AgipMNPV plaque isolates [17] were isolated from 3rd-passage infected-cell medium using the procedures above, but were not subject to purification by equilibrium gradient centrifugation.…”

Section: Viral Dna Isolationmentioning

confidence: 99%

“…Aliquots of infected-cell medium were harvested at 24, 48, and 72 h p. i. and titered by plaque assay [17]. Polyhedra were purified from cells at 96 h p. i. following the procedure of O'Reilly et al [15] and counted with a hemocytometer.…”

Section: Quantitation Of Viral Growthmentioning

confidence: 99%

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Genomic sequence analysis of the Illinois strain of the Agrotis ipsilon multiple nucleopolyhedrovirus

Harrison

2008

Virus Genes

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The Agrotis ipsilon multiple nucleopolyhedrovirus (AgipMNPV) is a group II nucleopolyhedrovirus (NPV) from the black cutworm, A. ipsilon, with potential as a biopesticide to control infestations of cutworm larvae. The genome of the Illinois strain of AgipMNPV was completely sequenced. The AgipMNPV genome was 155,122 nt in size and contained 163 open reading frames (ORFs), including 61 ORFs found among all lepidopteran baculoviruses sequenced to date. Phylogenetic inference placed AgipMNPV in a clade with group II NPVs isolated from larvae of Agrotis and Spodoptera species. Though closely related to the Agrotis segetum NPV (AgseNPV), AgipMNPV was found to be missing 15 ORFs present in the AgseNPV genome sequence, including two of the three AgseNPV enhancin genes. Remarkably few polymorphisms were identified in the AgipMNPV sequence even though an uncloned field isolate of this virus was sequenced. A genotype characterized by a 128-bp deletion in the ecdysteroid UDP-glucosyltransferase gene (egt) was identified in the AgipMNPV field isolate and among clonal isolates of AgipMNPV. The deletion in egt was not associated with differences in budded virus or occluded virus production among AgipMNPV clones in cell culture.

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Section: Genetic Variation In the Agipmnpv Illinois Isolatementioning

confidence: 88%

Section: Viral Dna Isolationmentioning

confidence: 99%

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Genomic sequence analysis of the Illinois strain of the Agrotis ipsilon multiple nucleopolyhedrovirus

Harrison

2008

Virus Genes

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“…AgseNPV-containing haemolymph was collected and prepared, as described above for S. exigua larvae, from A. segetum larvae infected with AgseNPV at a dose of 10 3 OBs per larva. The virus titre was determined in AiE1611T cells (Harrison & Lynn, 2008). Injected larvae were reared individually and three to five larvae were collected per treatment at 0, 12, 24, 48, 72, 96 and 168 h p.i.…”

Section: Methodsmentioning

confidence: 99%

Host-range expansion of Spodoptera exigua multiple nucleopolyhedrovirus to Agrotis segetum larvae when the midgut is bypassed

Jakubowska

Lynn

Herrero

et al. 2009

Journal of General Virology

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Given the high similarity in genome content and organization between Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) and Agrotis segetum nucleopolyhedrovirus (AgseNPV), as well as the high percentages of similarity found between their 30 core genes, the specificity of these NPVs was analysed for the respective insect hosts, S. exigua and A. segetum. The LD 50 for AgseNPV in second-instar A. segetum larvae was 83 occlusion bodies per larva and the LT 50 was 8.1 days. AgseNPV was orally infectious for S. exigua, but the LD 50 was 10 000-fold higher than for SeMNPV. SeMNPV was not infectious for A. segetum larvae when administered orally, but an infection was established by injection into the haemocoel. Bypassing midgut entry by intrahaemocoelic inoculation suggested that the midgut is the major barrier in A. segetum larvae for infection by SeMNPV. Delayed-early genes of SeMNPV are expressed in the midgut of A. segetum larvae after oral infections, indicating that the virus is able to enter midgut epithelial cells and that it proceeds through the first phases of the infection process. The possible mechanisms of A. segetum resistance to SeMNPV in per os infections are discussed.

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“…The black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), is found in many regions worldwide and feeds on a wide range of plants (Harrison & Lynn 2008). All instars of the larval stage of A. ipsilon feed on leaves of seedlings of nearly all vegetable and field crops, most importantly on crops including tobacco, cotton, tomato, potato, cabbage, barley and oats (Rings et al 1975).…”

Section: Introductionmentioning

confidence: 99%

Virulence and Pathogenicity of Recombinant Nucleopolyhedroviruses Expressing a Scorpion Toxin and an Insect Enzyme onAgrotis ipsilon(Lepidoptera: Noctuidae) Larvae

El-Sheikh¹

2014

African Entomology

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New cell lines derived from the black cutworm, Agrotis ipsilon, that support replication of the A. ipsilon multiple nucleopolyhedrovirus and several group I nucleopolyhedroviruses

Cited by 17 publications

References 48 publications

Genomic sequence analysis of the Illinois strain of the Agrotis ipsilon multiple nucleopolyhedrovirus

Genomic sequence analysis of the Illinois strain of the Agrotis ipsilon multiple nucleopolyhedrovirus

Host-range expansion of Spodoptera exigua multiple nucleopolyhedrovirus to Agrotis segetum larvae when the midgut is bypassed

Virulence and Pathogenicity of Recombinant Nucleopolyhedroviruses Expressing a Scorpion Toxin and an Insect Enzyme onAgrotis ipsilon(Lepidoptera: Noctuidae) Larvae

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