New Chemovariety of <i>Lippia alba</i> From Colombia: Compositional Analysis of the Volatile Secondary Metabolites and Some <i>in vitro</i> Biological Activities of the Essential Oil From Plant Leaves

Muñoz-Acevedo, Amner; González, Martha Letícia Gaeta; Rodríguez, Jesús Daniel Santos; Moya, Yurina Sh De

doi:10.1177/1934578x19862905

Cited by 5 publications

(4 citation statements)

References 57 publications

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“…The other compounds that varied significantly between individuals were generally less intense. As chemotypes have been detected for other medicinal species of Lamiaceae [29][30][31], further investigation of the P. neochilus volatile profile through a population-based study could be undertaken to define if there are also different chemotypes in this species. e155 ▶table 2 One-way ANOVA with Tukey's HSD post hoc test of P. neochilus Schltr.…”

Section: Discussionmentioning

confidence: 99%

Effect of Environmental Factors on Plectranthus Neochilus Volatile Composition: A GC-MS-Based Metabolomics Approach

Galbiatti

Pinheiro

Antunes

et al. 2021

Planta Medica International Open

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Plectranthus neochilus Schltr. is an aromatic species, commonly used for digestive, antispasmodic, and analgesic purposes. Although many studies have reported the chemical composition of its essential oil, variations in the volatile profile were observed, which may be due to multiple factors linked to growth and field conditions. In order to detect metabolic variations in this species, we employed a GC-MS-based untargeted metabolomics approach analyzing samples of four P. neochilus individuals collected over a year. From all analyses, 24 mass features were detected and 21 were identified according to their respective chromatographic peaks. All features varied among samples, particularly (2E)-hexenal, 3-octanone and δ-3-carene, which showed the highest coefficient of variation percentage in our study. Although the four individuals presented the same peaks in the chromatograms, significant differences in the intensity of specific mass features were detected between individuals throughout the year. Time of sampling did not affect P. neochilus volatile composition; the chemical profile remained constant throughout the day. Seasonal trends were observed for the species. Winter months coincided with a drop in the intensity of most components. Air temperature showed a positive correlation with some feature intensities, while myrcene and α-thujene resulted in a positive and a negative correlation with rainfall, respectively. This study was the first attempt to correlate metabolic variation and environmental factors in P. neochilus. Our approach was successful in identifying the composition and variation of the headspace volatiles of P. neochilus leaves.

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Section: Discussionmentioning

confidence: 99%

Effect of Environmental Factors on Plectranthus Neochilus Volatile Composition: A GC-MS-Based Metabolomics Approach

Galbiatti

Pinheiro

Antunes

et al. 2021

Planta Medica International Open

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“…Cytotoxic, acetylcholinesterase enzyme inhibition and repellent capabilities. The cytotoxicity (on human erythrocytes and lymphocytes, and Hep-2 cell line) along with acetylcholinesterase enzyme inhibition assays were carried out based on the methodology described by Muñoz-Acevedo et al [ 60 ]. The repellent test was carried out based on the preferred area procedure reported by Tapondjou et al [ 61 ].…”

Section: Methodsmentioning

confidence: 99%

Volatile Metabolites of Piper eriopodon (Miq.) C.DC. from Northern Region of Colombia and Assessment of In Vitro Bioactivities of the Leaf Essential Oil

et al. 2023

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Piper eriopodon is one of the Piper species found in the Sierra Nevada de Santa Marta, and the species has been reported with different compositions of their essential oils (EO). In this study, the volatile fractions/essential oil (by HS-SPME/SDE/MWHD-GC–MS/1H-NMR) of different parts from the plant were characterized, and assessments of the in vitro bio-properties of the leaf EO were conducted. The results indicated the following: (i) in the volatile fractions were β-caryophyllene (~23%)/myrcene (~20%) (inflorescences) and β-caryophyllene (~43%)/β-selinene (~20%) (leaves) using HS-SPME; myrcene (~31%)/β-pinene (~23%) (inflorescences), gibbilimbol B (~60%) (fruits) and gibbilimbol B (~46%)/β-caryophyllene (~11%) (leaves) through SDE; (ii) leaf EO contained gibbilimbol B (~72%), confirmed with 1H-NMR; (iii) the cytotoxic values (µg/mL) in erythrocytes/lymphocytes/Hep-2 were HC50: 115 ± 3 (eryth.), LC50: 71 ± 4 (lymph.) and LC50: 33 ± 2 (cell-line); (iv) the antibacterial susceptibilities (ϕ inh. zone, mm; 4–16 µg EO) were 22.5 ± 0.4–97 ± 4 (Staphylococcus aureus), 23 ± 2–77 ± 4 (Escherichia coli) and 17 ± 1–48 ± 3 (Listeria monocytogenes); (v) the TAA value was 2249 ± 130 mmol Trolox®/kg; (vi) the IC50 value was 13±1 µg/mL (AChE) with 20 ± 0–37 ± 6% repellency (2–4 h, Sitophilus zeamais). Thus, the EO of P. eriopodon leaves from northern Colombia could be a promising species for sustainable exploitation in the future due to its outstanding bioactivities.

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“…The EOs were isolated from fresh (or dried) parts of the plants (200-300 g) through hydrodistillation (with a modified Clevenger-type apparatus with Dean-Stark reservoir) assisted by microwave heating (Whirpool ® (Benton Harbor, MI, USA), domestic oven model WMS07ZDHS, operated at 700 W) for 1 hour in four 15 min cycles. Once the EOs were obtained, they were decanted, dehydrated with anhydrous sodium sulfate, and analyzed using GC-MS [145]. The chemical analysis of the EOs was then carried out using a Trace 1310 GC coupled to an ISQ Series MS (Thermo Fisher Scientific, Waltham, MA, USA), with a split/splitless inlet (split ratio of 10:1) and a liquid autosampler (AI/AS 1310 Series, Thermo Fisher Scientific).…”

Section: Isolation Of Essential Oils and Gc-ms Analysismentioning

confidence: 99%

“…Moreover, the Rxi ® -1ms column (30 m × 0.25 mm ID × 0.5 µm df, Restek Co., Centre County, PA, USA) was suitable for separation by individual constituents. The temperature programming of the GC oven was executed according to Muñoz Acevedo et al [145]. Chromatographic/mass spectra data were processed/analyzed using Thermo Xcalibur TM (Version 2.2 SP1.48) along with AMDIS (Build 130.53, Version 2.70) software.…”

Section: Isolation Of Essential Oils and Gc-ms Analysismentioning

confidence: 99%

The Repellent Capacity against Sitophilus zeamais (Coleoptera: Curculionidae) and In Vitro Inhibition of the Acetylcholinesterase Enzyme of 11 Essential Oils from Six Plants of the Caribbean Region of Colombia

Muñoz-Acevedo,

González,

Alonso

et al. 2024

Molecules

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The repellent capacity against Sitophilus zeamais and the in vitro inhibition on AChE of 11 essential oils, isolated from six plants of the northern region of Colombia, were assessed using a modified tunnel-type device and the Ellman colorimetric method, respectively. The results were as follows: (i) the degree of repellency (DR) of the EOs against S. zeamais was 20–68% (2 h) and 28–74% (4 h); (ii) the IC50 values on AChE were 5–36 µg/mL; likewise, the %inh. on AChE (1 µg/cm3 per EO) did not show any effect in 91% of the EO tested; (iii) six EOs (Bursera graveolens—bark, B. graveolens—leaves, B. simaruba—bark, Peperomia pellucida—leaves, Piper holtonii (1b*)—leaves, and P. reticulatum—leaves) exhibited a DR (53–74%) ≥ C+ (chlorpyrifos—61%), while all EOs were less active (8–60-fold) on AChE compared to chlorpyrifos (IC50 of 0.59 µg/mL). Based on the ANOVA/linear regression and multivariate analysis of data, some differences/similarities could be established, as well as identifying the most active EOs (five: B. simaruba—bark, Pep. Pellucida—leaves, P. holtonii (1b*)—leaves, B. graveolens—bark, and B. graveolens—leaves). Finally, these EOs were constituted by spathulenol (24%)/β-selinene (18%)/caryophyllene oxide (10%)—B. simaruba; carotol (44%)/dillapiole (21%)—Pep. pellucida; dillapiole (81% confirmed by 1H-/13C-NMR)—P. holtonii; mint furanone derivative (14%)/mint furanone (14%)—B. graveolens—bark; limonene (17%)/carvone (10%)—B. graveolens—leaves.

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New Chemovariety of Lippia alba From Colombia: Compositional Analysis of the Volatile Secondary Metabolites and Some in vitro Biological Activities of the Essential Oil From Plant Leaves

Cited by 5 publications

References 57 publications

Effect of Environmental Factors on Plectranthus Neochilus Volatile Composition: A GC-MS-Based Metabolomics Approach

Effect of Environmental Factors on Plectranthus Neochilus Volatile Composition: A GC-MS-Based Metabolomics Approach

Volatile Metabolites of Piper eriopodon (Miq.) C.DC. from Northern Region of Colombia and Assessment of In Vitro Bioactivities of the Leaf Essential Oil

The Repellent Capacity against Sitophilus zeamais (Coleoptera: Curculionidae) and In Vitro Inhibition of the Acetylcholinesterase Enzyme of 11 Essential Oils from Six Plants of the Caribbean Region of Colombia

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