New chiral derivatization reagent for the resolution of amino acids as diastereomers by TLC and HPLC

Nishi, Hiroyuki; Ishii, Kazuhiro; Taku, Ken-ichi; Shimizu, Reiji; Tsumagari, Nobuchika

doi:10.1007/bf02321273

Cited by 17 publications

(2 citation statements)

References 12 publications

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“…LOD of the minor enantiomer at S/N =3 was 0.1% [6]. -NSPC was useful for the separation of 18 DL-amino acids by TLC and adsorption HPLC with a Zorbax Sil column (4.6 mm ID x 15 cm) [7].…”

Section: Enantiomer Separation Of Pharmaceuticals By Hplc and Its Appmentioning

confidence: 99%

Development of Fast and Selective Analytical Methods of Pharmaceuticals and Herbal Medicines by High-Performance Liquid Chromatography and Capillary Electrophoresis

Nishi

2021

Chromatography

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Pharmaceuticals are essential for the medical treatment of various diseases. From the herbal medicines (crude drugs), synthetic medicines to biopharmaceuticals, to assure its efficacy and safety, the production procedures and the quality control methods of pharmaceuticals are important. These are strictly regulated by Good Manufacturing Practice (GMP) and various guidelines. The key is to develop the "Specifications and testing methods" of the pharmaceutical scientifically and rationally according to the production process. There are many testing items such as identity tests, purity tests, assay, etc. in "Specifications and testing methods". Among them, assay and purity test are core testing items. Optical purity evaluation is required for the single enantiomer pharmaceutical. Chromatographic methods and its detection methods are useful for the purpose. High-performance liquid chromatography (HPLC), thin layer chromatography (TLC) and gas chromatography (GC) are commonly used because the target analyses are almost a mixture. In this review, results of the development of the quality evaluation methods of relatively low molecular pharmaceuticals and herbal medicines by HPLC and capillary electrophoresis (CE) we have been investigated are summarized.

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Section: Enantiomer Separation Of Pharmaceuticals By Hplc and Its Appmentioning

confidence: 99%

Development of Fast and Selective Analytical Methods of Pharmaceuticals and Herbal Medicines by High-Performance Liquid Chromatography and Capillary Electrophoresis

Nishi

2021

Chromatography

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“…A new chiral reagent, NSP-C1, was synthesized and used to derivatize amino acid diastereomers, and the resulting amides were resolved by TLC (114). TLC on Chiralplates with acetonitrile-methanol-water (4:1:1) mobile phase was used to evaluate reaction products in the synthesis of modified phenylalanine and tyrosine derivatives and for the determination of the amino acid configuration of synthetic peptide analogues prepared starting from the racemic aromatic amino acids (115).…”

Section: Applicationsmentioning

confidence: 99%

Planar chromatography

Sherma¹

1992

Anal. Chem.

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Jo*eph Sherma received a B.S. In chemistry from Upsala College, East Orange. NJ. In 1955 and a Ph D. in analytical chemistry from Rudgers University in 1958. His thesis research In Ion exchange chromatography was under the direction of the late Wm. Rieman III. Dr. Sherma joined the faculty of Lafayette College in Sept 1958 and Is presently John D. & Frances H. Larkin Professor and Head of the Department of Chemistry and Is in charge of three courses in analytical chemistry. Dr. Sherma independently and with others has written or edited over 350 papers, chapters, books, and reviews covering chromatographic and analytical methods. His current research interests are In quantitative TLC. mainly applied to lipid analysis, pesticide residues, and food additives. He is editor for residues and elements of the Journal of AOAC International. by continuing to offer periodic instrumental TLC training courses.Also mentioned is the lack of text books on modern TLC. To help overcome this shortage of information, Sherma and Fried edited a comprehensive Handbook of Thin Layer Chromatography (5), which contains 1047 pages and includes 13 chapters on the principles and practice of TLC and 18 chapters on applications written by over 40 expert authors from around the world.Chromatographic analysis plays a vital role in forensic science. A feature article in Analytical Chemistry (6) on analytical methods for detecting fraudulent documents contained a number of examples of the use of qualitative and quantitative TLC in this area. GENERAL CONSIDERATIONSA. Books, Reviews, History, and Student Experiments.A general book on TLC (Al) and books covering planar chromatography in the life sciences (A2) and detection reagents and methods (A3) were published. A new volume on carbohydrates in the CRC Handbook of Chromatography series was published in 1991; this handbook contains considerable TLC, HPLC, and GC data, as well as information on sample preparation and solute detection (A4).The following aspects of planar chromatography were reviewed: resolution and separation capacity (A5), Chromarod-Iatroscan TLC-FID (A6), enantiomeric separations (A7, A8), comparisons of TLC and HPLC chiral separations (A9), applications to diagnosis of inborn errors of metabolism (A10), pharmaceutical analysis (All), clinical analysis (A12), analysis

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Chiral separation by high performance liquid chromatography. I. Review on indirect separation of enantiomers as diastereomeric derivatives using ultraviolet, fluorescence and electrochemical detection

Srinivas

Igwemezie

1992

Biomedical Chromatography

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The increased attention on the therapeutic implications of stereoisomerism has provided an impetus for the development of analytical methods for enantiomeric separation. The indirect method of separation of enantiomers as diastereomers using high performance liquid chromatography (HPLC) has emerged as an efficient and versatile approach. This is due mainly to the availability of numerous chiral derivatization reagents (CDRs). This article reviews CDRs useful for the development of an indirect HPLC method using ultraviolet, fluorescence and electrochemical detection. In addition, factors crucial for the development of the indirect method are discussed.

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New chiral derivatization reagent for the resolution of amino acids as diastereomers by TLC and HPLC

Cited by 17 publications

References 12 publications

Development of Fast and Selective Analytical Methods of Pharmaceuticals and Herbal Medicines by High-Performance Liquid Chromatography and Capillary Electrophoresis

Development of Fast and Selective Analytical Methods of Pharmaceuticals and Herbal Medicines by High-Performance Liquid Chromatography and Capillary Electrophoresis

Planar chromatography

Chiral separation by high performance liquid chromatography. I. Review on indirect separation of enantiomers as diastereomeric derivatives using ultraviolet, fluorescence and electrochemical detection

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