New class of antifungal agents: jasplakinolide, a cyclodepsipeptide from the marine sponge, Jaspis species

Scott, V R; Boehme, Richard; Matthews, Thomas R.

doi:10.1128/aac.32.8.1154

Cited by 84 publications

(58 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S6 in the supplemental material) isolated from sponge Jaspis sp. (71). This similarity suggests a shared underlying structure of biosynthetic proteins and/or gene clusters.…”

Section: Discussionmentioning

confidence: 97%

Genome Analysis of the Fruiting Body-Forming Myxobacterium Chondromyces crocatus Reveals High Potential for Natural Product Biosynthesis

Zaburannyi

Bunk

Maier

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Here, we report the complete genome sequence of the type strain of the myxobacterial genus Chondromyces, Chondromyces crocatus Cm c5. It presents one of the largest prokaryotic genomes featuring a single circular chromosome and no plasmids. Analysis revealed an enlarged set of tRNA genes, along with reduced pressure on preferred codon usage compared to that of other bacterial genomes. The large coding capacity and the plethora of encoded secondary metabolite biosynthetic gene clusters are in line with the capability of Cm c5 to produce an arsenal of antibacterial, antifungal, and cytotoxic compounds. Known pathways of the ajudazol, chondramide, chondrochloren, crocacin, crocapeptin, and thuggacin compound families are complemented by many more natural compound biosynthetic gene clusters in the chromosome. Whole-genome comparison of the fruiting-bodyforming type strain (Cm c5, DSM 14714) to an accustomed laboratory strain which has lost this ability (nonfruiting phenotype, Cm c5 fr؊) revealed genetic changes in three loci. In addition to the low synteny found with the closest sequenced representative of the same family, Sorangium cellulosum, extensive genetic information duplication and broad application of eukaryotic-type signal transduction systems are hallmarks of this 11.3-Mbp prokaryotic genome. Myxobacteria represent a branch of Deltaproteobacteria with exceptionally large and GC-rich genomes. Usually isolated from soil, these bacteria are best known for a complex development cycle and as prolific producers of secondary metabolites exhibiting a broad variety of biological activities (1). The chemical diversity of the produced compounds has motivated scientists to study more myxobacterial strains, with an emphasis on phylogenetic variety (2, 3, 4). Several genomes of secondary metaboliteproducing myxobacteria have been completely sequenced (5, 6). They exhibit genome sizes ranging from 9.2 to 14.7 Mbp, including the currently largest known bacterial genome of Sorangium cellulosum So0157-2 (7). The genomic diversity of myxobacteria is vast, and even strains of the same species may undergo genome reduction or expansion at an order of millions of base pairs (6, 7). Such diversity coincides with deviations in the genomic content of secondary metabolite biosynthetic gene clusters of known genomes, ranging from a prediction of 19 such clusters in Sandaracinus amylolyticus DSM 53668 to an estimated 38 clusters in Sorangium cellulosum So0157-2. Therefore, to better understand the biosynthetic capabilities of the myxobacteria and the relative frequency of secondary metabolites encoded in their genomes, more representatives are required to be sequenced. We here report our analysis of the genome sequence of Chondromyces crocatus Cm c5, the first representative of the genus Chondromyces belonging to the family of the Sorangiineae. MATERIALS AND METHODSGenome sequencing. The genome sequence of Chondromyces crocatus Cm c5 was determined through a combination of next-and thirdgeneration technologies available at the Helmho...

show abstract

“…S6 in the supplemental material) isolated from sponge Jaspis sp. (71). This similarity suggests a shared underlying structure of biosynthetic proteins and/or gene clusters.…”

Section: Discussionmentioning

confidence: 97%

Genome Analysis of the Fruiting Body-Forming Myxobacterium Chondromyces crocatus Reveals High Potential for Natural Product Biosynthesis

Zaburannyi

Bunk

Maier

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…compounds isolated from sponges are the chondramides [18], which resemble jaspamide/jasplakinolide from the sponge Jaspis johnstoni [19], and saframycin [20] which is chemically related to renieramycin from the sponge Reniera sp. [21].…”

Section: Biological Propertiesmentioning

confidence: 99%

Cruentaren, a New Antifungal Salicylate-Type Macrolide from Byssovorax cruenta (Myxobacteria) with Inhibitory Effect on Mitochondrial ATPase Activity

et al. 2006

View full text Add to dashboard Cite

The novel macrolide cruentaren A was produced at levels up to 3.2 mg/liter by cultures of the myxobacterium Byssovorax cruenta. The new compound strongly inhibited the growth of yeasts and filamentous fungi and showed high cytotoxicity against L929 mouse fibroblast cells. A minor co-metabolite of cruentaren A, named cruentaren B, and identified as a six-membered lactone isomer of cruentaren A, showed only marginal cytotoxicity and no antifungal activity. Cruentaren A inhibited F 0 F 1 mitochondrial ATP-hydrolysis in submitochondrial particles of yeasts and beef heart.

show abstract

“…7A). To determine whether the increased adhesion requires reorganization of filamentous actin, we applied the cell permeable reagent jasplakinolide, which stabilizes actin filaments (Scott et al, 1988;Visegrady et al, 2005), and found that pretreatment with jasplakinolide reduced adhesion to netrin 1 and blocked the effects of inhibiting RhoA signaling (Fig. 7A).…”

Section: Research Articlementioning

confidence: 99%

Rho inhibition recruits DCC to the neuronal plasma membrane and enhances axon chemoattraction to netrin 1

et al. 2008

View full text Add to dashboard Cite

Molecular cues, such as netrin 1, guide axons by influencing growth cone motility. Rho GTPases are a family of intracellular proteins that regulate the cytoskeleton, substrate adhesion and vesicle trafficking. Activation of the RhoA subfamily of Rho GTPases is essential for chemorepellent axon guidance; however, their role during axonal chemoattraction is unclear. Here, we show that netrin 1, through its receptor DCC, inhibits RhoA in embryonic spinal commissural neurons. To determine whether netrin 1-mediated chemoattraction requires Rho function, we inhibited Rho signaling and assayed axon outgrowth and turning towards netrin 1. Additionally, we examined two important mechanisms that influence the guidance of axons to netrin 1: substrate adhesion and transport of the netrin receptor DCC to the plasma membrane. We found that inhibiting Rho signaling increased plasma membrane DCC and adhesion to substrate-bound netrin 1, and also enhanced netrin 1-mediated axon outgrowth and chemoattractive axon turning. Conversely, overexpression of RhoA or constitutively active RhoA inhibited axonal responses to netrin 1. These findings provide evidence that Rho signaling reduces axonal chemoattraction to netrin 1 by limiting the amount of plasma membrane DCC at the growth cone, and suggest that netrin 1-mediated inhibition of RhoA activates a positive-feedback mechanism that facilitates chemoattraction to netrin 1. Notably, these findings also have relevance for CNS regeneration research. Inhibiting RhoA promotes axon regeneration by disrupting inhibitory responses to myelin and the glial scar. By contrast, we demonstrate that axon chemoattraction to netrin 1 is not only maintained but enhanced, suggesting that this might facilitate directing regenerating axons to appropriate targets.

show abstract

New class of antifungal agents: jasplakinolide, a cyclodepsipeptide from the marine sponge, Jaspis species

Cited by 84 publications

References 10 publications

Genome Analysis of the Fruiting Body-Forming Myxobacterium Chondromyces crocatus Reveals High Potential for Natural Product Biosynthesis

Genome Analysis of the Fruiting Body-Forming Myxobacterium Chondromyces crocatus Reveals High Potential for Natural Product Biosynthesis

Cruentaren, a New Antifungal Salicylate-Type Macrolide from Byssovorax cruenta (Myxobacteria) with Inhibitory Effect on Mitochondrial ATPase Activity

Rho inhibition recruits DCC to the neuronal plasma membrane and enhances axon chemoattraction to netrin 1

Contact Info

Product

Resources

About