About 8 million tons of jujube pomace arise within a year in China. Bioconversion of food processing residues is receiving increased attention because these residual materials represent a possible and utilizable resource for conversion into useful products. To explore the application of Chinese jujube (Ziziphus jujube Mill.) seeds protein concentrate (ZPI ) in food industry, its physiochemical and functional properties were analyzed in this study. The free sulfhydryl content of ZPI is 7.1 × 10 _ 6 mol/g of protein at pH 8.0. Differential scanning calorimetry analyses showed that ZPI had only one endothermic peak with denaturation temperature of about 91℃. Amino acid analyses revealed that ZPI exhibited high levels of glutamic acid, aspartic acid and arginine. The least gelation concentration (LGC) of ZPI is ~8% at pH 7.0, whereas the protein solubility, emulsifying activity, emulsion stability, and waterholding capacity of ZPI were much lower than those of soybean protein concentrate (SPI). These results suggest that ZPI could be used as a valuable source of protein and gel.Keywords: Chinese jujube seed protein concentrate (ZPI), amino acid composition, physiochemical property, functional property
IntroductionJujube (Zizyphus jujube Mill.), which is mainly distributed in tropical and subtropical regions of the world, is a tree of the Rhamnaceae family (Li et al., 2007;Mukhtar et al., 2004;Su et al., 2005). Jujube is a popular and profitable fruit, and is much admired due to its high nutritional value. Thus, Chinese jujube has been commonly used in traditional Chinese medicine and also used as food for thousands of years. The Chinese share of world jujube production is about 90% and its production has increased in the last ten years due to the demands for food and pharmaceutical applications (Li et al., 2005;Su et al., 2005), with a total yield of 36 million tons in 2008 (MOA, 2009. About 80% of the annual Chinese jujube production has been consumed in fresh, dried forms and juice, and therefore ~8 million tons of jujube pomace arise annually. These wastes pose potential disposal and pollution problems along with loss of valuable biomass and nutrients.Bioconversion of food processing residues is receiving increased attention because these residues represent a possible and utilizable resource for conversion into useful products. In this context, the utilization of wastes of fruit processing as a source of functional ingredients is a promising field (Schieber et al., 2001
Material and MethodsMaterials Chinese jujube seeds (Ziziphus jujube Mill.) were obtained from Henan Haoxiangni Co., Ltd. Dried soybean (Glycine max) seeds were obtained from the local market. All reagents used in the present study were of analytical grade or better grade.Preparation of seed protein concentrate About 1.0 kg of jujube seeds or soybean seeds were soaked in distilled water overnight and homogenized in four volumes of an aqueous buffer (50 mM phosphate buffered saline (PBS), 1% polyvinylpolypyrrolidone, pH 9.0) using a Waring blender...