New Contrast Stain for the Rapid Diagnosis of Dermatophytosis and Pityriasis Versicolor

Fonseka, Sanjeewani; Lim, Christopher Sh; Bandara, Upendra N.; Dissanayake, Manel

doi:10.1309/lm1bsuj6lm2opaae

Cited by 5 publications

(8 citation statements)

References 8 publications

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“…Immediate diagnosis of superficial mycoses is desirable as specific treatment can be initiated without delay. Fungal culture takes too long and is impractical for this purpose . The KOH wet mount using bright field microscopy is inexpensive and routinely used for the immediate diagnosis of fungal infections.…”

Section: Discussionmentioning

confidence: 99%

“…Fungal culture takes too long and is impractical for this purpose. 4 The KOH wet mount using bright field microscopy is inexpensive and routinely used for the immediate diagnosis of fungal infections. However, it does not produce a color contrast and requires considerable skill to interpret.…”

Section: Discussionmentioning

confidence: 99%

“…The diagnosis of superficial mycosis such as dermatophytosis and pityriasis versicolor is often done clinically. However, in more difficult cases, clinicians request laboratory confirmation . The laboratory diagnosis of dermatophytosis routinely involves direct microscopic examination of clinical specimens followed by in vitro culture techniques.…”

Section: Introductionmentioning

confidence: 99%

“…However, in more difficult cases, clinicians request laboratory confirmation. 4 The laboratory diagnosis of dermatophytosis routinely involves direct microscopic examination of clinical specimens followed by in vitro culture techniques. Microscopic identification of fungal elements is a rapid diagnostic method, but it lacks specificity and sensitivity with false negative results in up to 15% cases.…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of efficacy, rapidity of detection, and cost‐effectiveness of potassium hydroxide, calcofluor white, and Chicago sky blue stains in the diagnosis of dermatophytoses

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative study of efficacy, rapidity of detection, and cost‐effectiveness of potassium hydroxide, calcofluor white, and Chicago sky blue stains in the diagnosis of dermatophytoses

et al. 2016

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“…versicolor. [ 6 7 8 ] It contains 1% CSB and is used together with 10% KOH as the clearing agent. It stains the fungal hyphae and spores a contrast blue against a light purplish background of cellular debris.…”

Section: Introductionmentioning

confidence: 99%

A novel contrast stain for the rapid diagnosis of pityriasis versicolor: A comparison of Chicago Sky Blue 6B stain, potassium hydroxide mount and culture

Lodha

Poojary

2015

Indian J Dermatol

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Background:The mycological study of pityriasis versicolor is usually done by potassium hydroxide (KOH) mount and culture. However, KOH mount lacks a color contrast and requires a trained eye to interpret, while culture is difficult to perform, time consuming and has low sensitivity. Chicago Sky Blue 6B (CSB) is a new contrast stain that highlights the fungal hyphae and spores, blue against a purplish background.Aims and Objectives:This study was done to compare the utility of a novel contrast stain (CSB stain) with KOH mount and culture.Materials and Methods:Skin scrapings from the lesions of 100 clinically diagnosed cases of P. versicolor were subjected to (1) KOH mount and CSB stain for direct microscopic examination and (2) culture using Sabouraud's dextrose agar. The statistical analysis of CSB stain and culture was done using KOH mount as the reference method, as it is the most commonly performed and practical diagnostic test available for P. versicolor. An interrater reliability analysis using the Cohen's Kappa statistic was performed to determine consistency (agreement) among the different modalities.Observations and Results:Direct microscopy with CSB stain, KOH mount and mycological culture showed positive results in 98 (98%), 92 (92%) and 56 (56%) patients, respectively. Using KOH mount as the reference method, CSB stain had a sensitivity of 100% which was significantly higher than culture (60.9%). Statistically significant fair agreement was found between CSB stain and KOH mount (94% with κ=0.38, P < 0.001). Negligible agreement was found between CSB stain and culture (66%, κ=0.199, P = 0.001) as well as between KOH mount and culture (64%, κ=0.051, P = 0.107).Conclusion:CSB staining of skin scrapings is the most sensitive method for the diagnosis of pityriasis versicolor. Due to the distinct contrast provided by CSB, it is easy to perform, rapid and qualitatively superior to KOH mount.

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Parasitic Diseases in Aquaculture: Their Biology, Diagnosis and Control

Paladini

Longshaw

Gustinelli

et al. 2017

Diagnosis and Control of Diseases of Fish and Shellfish

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New Contrast Stain for the Rapid Diagnosis of Dermatophytosis and Pityriasis Versicolor

Cited by 5 publications

References 8 publications

Comparative study of efficacy, rapidity of detection, and cost‐effectiveness of potassium hydroxide, calcofluor white, and Chicago sky blue stains in the diagnosis of dermatophytoses

Comparative study of efficacy, rapidity of detection, and cost‐effectiveness of potassium hydroxide, calcofluor white, and Chicago sky blue stains in the diagnosis of dermatophytoses

A novel contrast stain for the rapid diagnosis of pityriasis versicolor: A comparison of Chicago Sky Blue 6B stain, potassium hydroxide mount and culture

Parasitic Diseases in Aquaculture: Their Biology, Diagnosis and Control

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